Penicillin sodium

2,4,6-Tri(2-pyridyl)-1,3,5-triazine (TPTZ), 1,1-Diphenyl-2-picrylhydrazyl Free (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), Nutrient Broth, Agar Powder, and Trypsin (1:250) are from Beijing Solarbio Science & Technology Co., Ltd. Pepsin (1:3000) from BioFroxx, Germany. Escherichia coli (E. coli) is used as standard quality control strain ATCC 25922 and Staphylococcus aureus (S. aureus) is used as standard quality control strain ATCC 25923. Penicillin sodium is from Shanghai yuanye Bio-Technology Co., Ltd, Lot: S15J7Y9073, mass fraction 97%. Gallic acid is from Chengdu Kelong Chemical Reagent Factory, mass fraction 99%. Gallic acid ethyl ester is from Chengdu Prefa Technology Development Co. Lot: PRF20090442, mass fraction 98%. Folin–Ciocalteu’s phenol reagent is a homemade laboratory product. Other chemical reagents are all of analytical purity grade. Methanol is chromatographically pure grade. Water is purified water.

In this experiment, an artificial vagina was used to collect goat semen, and sperm motility analysis was conducted on the collected semen. Six goat semen with sperm motility higher than 80% were mixed, and the mixed semen was diluted eight times. The diluted semen was stored at a constant temperature of 4°C and used for detection and analysis.
The components of semen diluent were as follows: SS 0.16 to 0.8 mg or CC 0.04 to 0.80 mg, vitamin E 4 mL, penicillin sodium 15,000 IU, streptomycin sulfate15,000 IU, which were all bought from Shanghai yuanye Bio-Technology Co., Ltd (Shanghai, China). Fructose 1.26 g and citric acid 1.72 g, which were all bought from Shanghai Macklin Biochemical Co., Ltd (Shanghai, China). Trimethyl aminomethane 3.53 g, vitamin C 0.5 g, bovine serum albumin 0.5 g, which were all bought from Beijing Solarbio Science & Technology Co., Ltd (Beijing, China). Fresh egg yolk 20 mL, double-distilled water up to 100 mL. Diluent components are classified as analytical or guaranteed grade reagents.