3 amino 1 propanol s3

1,5-pentanediol diacrylate (B5; Monomer-Polymer and Dajac Labs, Trevose, PA, USA), and 3-amino-1-propanol (S3; Alfa Aesar, Ward Hill, MA, USA) were combined in a 1:1.1 molar ratio of backbone to sidechain monomer and polymerized at 90°C under stirring for 24 h. The resulting acrylate-terminated polymer (B5S3) was dissolved in tetrahydrofuran, and 2-(3-aminopropylamino)ethanol (E6; Sigma-Aldrich, St. Louis, MO, USA) was added at a 10-fold molar excess. The end capping reaction was allowed to proceed for 1 h at room temperature (RT) under stirring. Endcapped PBAE polymer 536 was purified twice in diethyl ether to remove unreacted monomer and short oligomers and then dried under desiccant for approximately 48 h to remove traces of ether. PBAE 536 was dissolved in anhydrous DMSO and stored at −20°C with desiccant. Molecular weight was characterized by Gel Permeation Chromatography (GPC, Waters 2414 Refractive Index Detector, Milford, MA, USA).

1,5-Pentanediol diacrylate (B5) (Monomer-Polymer and Dajac Labs, Trevose, PA) and 3-amino-1-propanol (S3) (Alfa Aesar, Ward Hill, MA) were combined neat at a 1:1.1 ratio for a 1.5-g total mass and allowed to polymerize under stirring for 24 hours at 90°C. This polymer was then dissolved in anhydrous tetrahydrofuran. 2-(3-Aminopropylamino)ethanol (E6) (Sigma-Aldrich, St. Louis, MO) was added in 10-fold molar excess and stirred at room temperature (RT) for 1 hour. The resulting polymer was washed two times in diethyl ether and dried under vacuum for several days. The polymer was dissolved in anhydrous DMSO and stored with desiccant at −20°C at 100 mg/ml. Molecular weight was measured by GPC relative to polystyrene standards (Waters 2414 Refractive Index Detector, Milford, MA).