Anti-PGK (clone 22C5D8, Invitrogen), anti-mouse/human actin (clone Ab-5, BD biosciences), anti-Toxoplasma gondii actin39 (link), horseradish peroxidase-conjugated goat anti-rabbit Ig (Southern Biotech, cat. 4041-05), horseradish peroxidase-conjugated anti-mouse Ig (GE Healthcare, cat. NXA931), anti-TCRβ (clone H57, BD Pharmingen), anti-CD4 (clone GK1.5, ebiosciences), anti-CD19 (clone 1D4, BD Pharmingen), anti-TER119 (clone TER-119, BD Pharmingen), allophycocyanin-conjugated streptavidin (ebiosciences, cat. number 17-4317), phycoerythrin-conjugated streptavidin (Southern Biotech, cat. number 7100-09S). Propidium iodide (Sigma-Aldrich, cat. number P4864). B cell isolation kit (Miltenyi, cat. 130-090-862). For the experiments described here we used a modified form of sortase A from Staphylococcus aureus that lacks the first 59 residues, and with the following mutations: E105K/E108A40 (link) and P94R/D160N/D165A/K190E/K196T24 (link): Sortase A was produced and purified as described elsewhere20 (link). Enhancer33 (link) and VHH734 (link) were produced and purified as described elsewhere34 (link). Biotin-LPETG (Biotin- aminohexanoic acid-LPETGG) was produced by the MIT biopolymer facility through standard solid phase peptide synthesis. TAMRA-LPETG was produced as described elsewhere20 (link).
Anti cd19 clone 1d4
Manufactured by BD
Anti-CD19 (clone 1D4) is a monoclonal antibody that recognizes the CD19 antigen, a cell surface protein expressed on B cells. This antibody can be used for the identification and isolation of CD19-positive cells in a research setting.
Automatically generated - may contain errors
Lab products found in correlation
B cell isolation kit, by Miltenyi Biotec (2 mentions)
Propidium iodide, by Merck Group (2 mentions)
Anti cd4 clone gk1, by Thermo Fisher Scientific (2 mentions)
Anti pgk clone 22c5d8, by Thermo Fisher Scientific (2 mentions)
Anti mouse human actin clone ab 5, by BD (2 mentions)
Horseradish peroxidase conjugated anti mouse ig, by GE Healthcare (2 mentions)
Anti tcrβ clone h57, by BD (2 mentions)
Allophycocyanin conjugated streptavidin, by Thermo Fisher Scientific (2 mentions)
Phycoerythrin conjugated streptavidin, by Southern Biotech (2 mentions)
Horseradish peroxidase conjugated goat anti rabbit ig, by Southern Biotech (2 mentions)
Anti ter119 clone ter 119, by BD (2 mentions)
2 protocols using anti cd19 clone 1d4
1
Antibodies and Biomolecules for Cellular Analysis
2
Engineered Sortase A for Bioconjugation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Anti-PGK (clone 22C5D8, Invitrogen),
antimouse/human actin (clone Ab-5, BD biosciences), anti-Toxoplasma
gondii actin,39 (link) horseradish peroxidase-conjugated
goat antirabbit Ig (Southern Biotech, cat. 4041-05), horseradish peroxidase-conjugated
antimouse Ig (GE Healthcare, cat. NXA931), anti-TCRβ (clone
H57, BD Pharmingen), anti-CD4 (clone GK1.5, ebiosciences), anti-CD19
(clone 1D4, BD Pharmingen), anti-TER119 (clone TER-119, BD Pharmingen),
allophycocyanin-conjugated streptavidin (ebiosciences, cat. number
17-4317), phycoerythrin-conjugated streptavidin (Southern Biotech,
cat. number 7100-09S). Propidium iodide (Sigma-Aldrich, cat. number
P4864). B cell isolation kit (Miltenyi, cat. 130-090-862). For the
experiments described here, we used a modified form of sortase A from Staphylococcus aureus that lacks the first 59 residues,
and with the following mutations: E105 K/E108A40 (link) and P94R/D160N/D165A/K190E/K196T:24 (link) Sortase A was produced and purified as described elsewhere.20 (link) Enhancer33 (link) and VHH734 (link) were produced and purified as described elsewhere.34 (link) Biotin-LPETG (Biotin- aminohexanoic acid-LPETGG)
was produced by the MIT biopolymer facility through standard solid
phase peptide synthesis. TAMRA-LPETG was produced as described elsewhere.20 (link)
antimouse/human actin (clone Ab-5, BD biosciences), anti-Toxoplasma
gondii actin,39 (link) horseradish peroxidase-conjugated
goat antirabbit Ig (Southern Biotech, cat. 4041-05), horseradish peroxidase-conjugated
antimouse Ig (GE Healthcare, cat. NXA931), anti-TCRβ (clone
H57, BD Pharmingen), anti-CD4 (clone GK1.5, ebiosciences), anti-CD19
(clone 1D4, BD Pharmingen), anti-TER119 (clone TER-119, BD Pharmingen),
allophycocyanin-conjugated streptavidin (ebiosciences, cat. number
17-4317), phycoerythrin-conjugated streptavidin (Southern Biotech,
cat. number 7100-09S). Propidium iodide (Sigma-Aldrich, cat. number
P4864). B cell isolation kit (Miltenyi, cat. 130-090-862). For the
experiments described here, we used a modified form of sortase A from Staphylococcus aureus that lacks the first 59 residues,
and with the following mutations: E105 K/E108A40 (link) and P94R/D160N/D165A/K190E/K196T:24 (link) Sortase A was produced and purified as described elsewhere.20 (link) Enhancer33 (link) and VHH734 (link) were produced and purified as described elsewhere.34 (link) Biotin-LPETG (Biotin- aminohexanoic acid-LPETGG)
was produced by the MIT biopolymer facility through standard solid
phase peptide synthesis. TAMRA-LPETG was produced as described elsewhere.20 (link)
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