For MTT assays, a total of 1500 cells were seeded per well in a 96-well plate. MTT assay reagent (Beyotime, Shanghai, China) was added to each well and incubated for 2 h at 37 °C. Newly formed mitochondrial formazan crystals were dissolved by 100 μl DMSO and the absorbance at 490 nm of each well was read on a spectrophotometer. For colony formation assays, a total of 1000 cells were seeded per well in a 6-well plate. After cultured for 2 weeks, the colonies were fixed with 4% paraformaldehyde for 20 min at room temperature and then stained with 0.1% crystal violet. The number of colonies (>50 cells) was counted.
Mtt assay reagent
Manufactured by Beyotime
Sourced in China
The MTT assay reagent is a colorimetric assay used to measure the metabolic activity of cells. It is commonly used in cell proliferation and cytotoxicity studies.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mtt assay reagent
1
Cell Viability and Colony Formation Assays
2
Anticancer Potential of Seafood Protein
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A total of 10 kg S. constricta were obtained from Beimen market in Zhoushan City, (Zhejiang, China). The muscles were rapidly separated, and stored at −80°C. Trypsin, pepsin, papain, alcalase, acetonitrile and trifluoroacetic acid (TFA) were purchased from YTHX Biotechnology Co., Ltd. (Beijing, China; www.ythxbio.com ). The MTT assay reagent was purchased from Beyotime Institute of Biotechnology (Nanjing, China). All additional reagents used were of analytical grade. Hormone-dependent DU-145 and PC-3 cell lines were obtained from the China Cell Bank of the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China). The annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) Apoptosis Detection and cell cycle staining kits were obtained from YTHX Biotechnology Co., Ltd.
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