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Mouse anti keratin 8

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Mouse anti-Keratin 8 is a primary antibody that specifically binds to the keratin 8 protein. Keratin 8 is an intermediate filament protein that is expressed in simple and glandular epithelial cells. This antibody can be used for the detection and localization of keratin 8 in various cell and tissue types.

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Lab products found in correlation

Rabbit anti vimentin, by Cell Signaling Technology (2 mentions) Rabbit anti oct4, by Abcam (2 mentions) Mouse anti tra 1 60, by Thermo Fisher Scientific (2 mentions) Rat anti k8, by Developmental Studies Hybridoma Bank (2 mentions) Rabbit anti lamin b1, by Abcam (2 mentions) Mouse anti gigaxonin, by Santa Cruz Biotechnology (2 mentions) Mouse anti nestin, by Thermo Fisher Scientific (2 mentions) Mouse anti tra 1 81, by Thermo Fisher Scientific (2 mentions) Mouse anti vimentin, by Thermo Fisher Scientific (2 mentions) Rabbit anti lamin a c, by Santa Cruz Biotechnology (2 mentions) Rabbit anti sox2, by Thermo Fisher Scientific (2 mentions) Mouse anti gfap, by Merck Group (1 mentions) Rabbit anti gfap, by Agilent Technologies (1 mentions)

2 protocols using mouse anti keratin 8

1

Comprehensive Immunofluorescence and Western Blot Antibody Protocol

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The following primary antibodies and concentrations were used in this study: rabbit anti-GFAP (DAKO, Agilent, clone Z0334; IF 1:500, WB 1:10,000), mouse anti-GFAP (Sigma, clone GA5; IF 1:300), mouse anti-Gigaxonin (Santa Cruz Biotechnology, F3, WB 1:200), rabbit anti-Vimentin (Cell-Signaling Technology, D21H3, IF 1:100), mouse anti-Vimentin (Thermo Fisher Scientific, V9, WB 1:1000), mouse anti-Keratin 8 (Thermo Fisher Scientific, TS1, IF 1:100), rat anti-K8 (Developmental Studies Hybridoma Bank, Troma I, WB 1:5000), rabbit anti-Lamin A/C (Santa Cruz Biotechnology), rabbit anti-Lamin B1 (Abcam ab16048, IF 1:10,000, WB 1:10,000), mouse anti-Nestin (Thermo Fisher Scientific, 10C2, IF 1:200), mouse anti-NF-M/H (clone RMdO-20; IF 1:100), mouse anti-Tra-1-60 (Thermo Fisher Scientific, 41-1000, IF 1:300), mouse anti-Tra-1-81 (Thermo Fisher Scientific, 41-1100, IF 1:300), rabbit anti-Oct4 (Abcam, ab19857, IF 1:40), and rabbit anti-Sox2 (Thermo Fisher Scientific, 48-1400, IF 1:125). The following secondary antibodies and concentrations were used: Alexa 488- and Alexa 594-conjugated goat-, anti-mouse-, and rabbit-antibodies (Thermo Fisher Scientific, IF 1:500), and peroxidase-conjugated goat-, anti-mouse-, and rabbit-antibodies (Sigma, WB 1:5000).
Battaglia R.A., Faridounnia M., Beltran A., Robinson J., Kinghorn K., Ezzell J.A., Bharucha-Goebel D., Bönnemann C.G., Hooper J.E., Opal P., Bouldin T.W., Armao D, & Snider N.T. (2023). Intermediate filament dysregulation in astrocytes in the human disease model of KLHL16 mutation in giant axonal neuropathy (GAN). Molecular Biology of the Cell, 34(12), ar121.
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2

Comprehensive Antibody Panel for Cellular Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following primary antibodies and concentrations were used in this study: rabbit anti-GFAP (DAKO, Agilent, clone Z0334; IF 1:500, WB 1:10,000), mouse anti-GFAP (Sigma, clone GA5; IF 1:300), mouse anti-pSer13-GFAP (gift from Dr. Masaki Inagaki, clone KT13, IF 1:20), mouse anti-Gigaxonin (Santa Cruz Biotechnology, F3, WB 1:200), rabbit anti-Vimentin (Cell Signaling Technology, D21H3, IF 1:100), mouse anti-Vimentin (Thermo Fisher Scientific, V9, WB 1:1000), mouse anti-Keratin 8 (Thermo Fisher Scientific, TS1, IF 1:100), rat anti-K8 (Developmental Studies Hybridoma Bank, Troma I, WB 1:5000), rabbit anti-Lamin A/C (Santa Cruz Biotechnology), rabbit anti-Lamin B1 (Abcam ab16048, IF 1:10,000, WB 1:10,000), mouse anti-Nestin (Thermo Fisher Scientific, 10C2, IF 1:200), mouse anti-Tra-1–60 (Thermo Fisher Scientific, 41–1000, IF 1:300), mouse anti-Tra-1–81 (Thermo Fisher Scientific, 41–1100, IF 1:300), rabbit anti-Oct4 (Abcam, ab19857, IF 1:40), and rabbit anti-Sox2 (Thermo Fisher Scientific, 48–1400, IF 1:125). The following secondary antibodies and concentrations were used: Alexa 488- and Alexa 594-conjugated goat anti mouse and rabbit antibodies (Thermo Fisher Scientific, IF 1:500), and peroxidase-conjugated goat anti-mouse and rabbit antibodies (Sigma, WB 1:5000).
Battaglia R., Faridounnia M., Beltran A., Robinson J., Kinghorn K., Ezzell J.A., Bharucha-Goebel D., Bonnemann C., Hooper J.E., Opal P., Bouldin T.W., Armao D, & Snider N. (2023). Intermediate filament dysregulation and astrocytopathy in the human disease model of KLHL16 mutation in giant axonal neuropathy (GAN). bioRxiv.
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