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Bck fc488 50

Manufactured by Merck Group
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The BCK-FC488-50 is a compact, high-performance flow cytometry instrument designed for research and clinical applications. It features a 488 nm laser and 50 mW power output, enabling the detection and analysis of fluorescently labeled cells and particles. The instrument is capable of measuring forward scatter, side scatter, and up to 4 fluorescent parameters simultaneously.

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Lab products found in correlation

Novocyte advanteon flow cytometer, by Agilent Technologies (2 mentions)

2 protocols using bck fc488 50

1

Click-IT EdU Proliferation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
The Click-IT EdU protocol was used according to manufacturer instructions (Sigma-Aldrich #BCK-FC488-50). Briefly, MEFs were seeded at 50,000 cells per well in 6-well plates in DMEM supplemented with 10% FBS and 1% P/S, and allowed to attach overnight. The next day, cells were serum-starved for 5 h prior to compound addition for 24 h in fresh serum-free DMEM. Cells were then pulsed for 3 h with 10 μM EdU, followed by collection by trypsinization and fixation with 3.7% FA in PBS for 15 min in the dark, washed in 3% BSA and permeabilized in 1x saponin-based permeabilization buffer for 20 min in the dark. EdU was then detected using the FAM-azide assay cocktail for 30 min in the dark. Cells were washed twice in 1x saponin-based permeabilization buffer followed by analysis with flow cytometer (Novocyte Advanteon flow cytometer, Agilent). Gating strategy for flow cytometry is shown in Supplementary Fig. 2.
Gong G.Q., Bilanges B., Allsop B., Masson G.R., Roberton V., Askwith T., Oxenford S., Madsen R.R., Conduit S.E., Bellini D., Fitzek M., Collier M., Najam O., He Z., Wahab B., McLaughlin S.H., Chan A.E., Feierberg I., Madin A., Morelli D., Bhamra A., Vinciauskaite V., Anderson K.E., Surinova S., Pinotsis N., Lopez-Guadamillas E., Wilcox M., Hooper A., Patel C., Whitehead M.A., Bunney T.D., Stephens L.R., Hawkins P.T., Katan M., Yellon D.M., Davidson S.M., Smith D.M., Phillips J.B., Angell R., Williams R.L, & Vanhaesebroeck B. (2023). A small molecule PI3Kα activator for cardioprotection and neuroregeneration. Nature, 618(7963), 159-168.
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2

Click-IT EdU Proliferation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
The Click-IT EdU protocol was used according to manufacturer instructions (Sigma-Aldrich #BCK-FC488-50). Briefly, MEFs were seeded at 50,000 cells per well in 6-well plates in DMEM supplemented with 10% FBS and 1% P/S, and allowed to attach overnight. The next day, cells were serum-starved for 5 h prior to compound addition for 24 h in fresh serum-free DMEM. Cells were then pulsed for 3 h with 10 μM EdU, followed by collection by trypsinization and fixation with 3.7% FA in PBS for 15 min in the dark, washed in 3% BSA and permeabilized in 1x saponin-based permeabilization buffer for 20 min in the dark. EdU was then detected using the FAM-azide assay cocktail for 30 min in the dark. Cells were washed twice in 1x saponin-based permeabilization buffer followed by analysis with flow cytometer (Novocyte Advanteon flow cytometer, Agilent). Gating strategy for flow cytometry is shown in Supplementary Fig. 2.
Gong G.Q., Bilanges B., Allsop B., Masson G.R., Roberton V., Askwith T., Oxenford S., Madsen R.R., Conduit S.E., Bellini D., Fitzek M., Collier M., Najam O., He Z., Wahab B., McLaughlin S.H., Chan A.E., Feierberg I., Madin A., Morelli D., Bhamra A., Vinciauskaite V., Anderson K.E., Surinova S., Pinotsis N., Lopez-Guadamillas E., Wilcox M., Hooper A., Patel C., Whitehead M.A., Bunney T.D., Stephens L.R., Hawkins P.T., Katan M., Yellon D.M., Davidson S.M., Smith D.M., Phillips J.B., Angell R., Williams R.L, & Vanhaesebroeck B. (2023). A small molecule PI3Kα activator for cardioprotection and neuroregeneration. Nature, 618(7963), 159-168.
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