Mouse livers were collected, fixed overnight in 4% paraformaldehyde in 1× PBS, embedded in paraffin, and sectioned at 5 μm. Sections were stained with hematoxylin and eosin for histological analysis. Sirius red (Abcam ab150681) staining and immunohistochemistry were performed according to the manufacturers’ protocols. Primary antibodies used for immunohistochemistry were rabbit anti-Ki67 (1:500; Novocastra NCL-Ki67p), anti-cleaved Caspase-3 (1:100; Cell Signaling 9661), anti-PanCK (1:200; DAKO Z0622), anti-Sox9 (1:200; Millipore AB5535), and anti-α-SMA (1:200; Sigma C6198). For Ki67, the signals were developed using the ABC kit purchased from Vector Laboratories according to the manufacturer's suggestions. Cy3-conjugated goat antirabbit secondary antibodies (Molecular Probes) were used for immunofluorescence.
Cy3 conjugated goat antirabbit secondary antibodies
Manufactured by Thermo Fisher Scientific
Sourced in United States
Cy3-conjugated goat antirabbit secondary antibodies are a type of laboratory reagent used in various immunoassay techniques. They are designed to bind to primary antibodies raised in rabbits, allowing for the detection and visualization of target antigens in samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti panck, by Agilent Technologies (1 mentions)
Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Ab5535, by Merck Group (1 mentions)
Ncl ki67p, by Cell Signaling Technology (1 mentions)
Abc kit, by Vector Laboratories (1 mentions)
Anti sox9, by Merck Group (1 mentions)
Anti α sma, by Merck Group (1 mentions)
Anti ph3 antibody, by Cell Signaling Technology (1 mentions)
Anti actin antibody, by MP Biomedicals (1 mentions)
Alexa fluor 405 and 488 conjugated streptavidin, by Thermo Fisher Scientific (1 mentions)
Gsl 1 isolectin b4, by Vector Laboratories (1 mentions)
Anti enos antibody, by BD (1 mentions)
2 protocols using cy3 conjugated goat antirabbit secondary antibodies
1
Histological Analysis of Mouse Liver
2
Immunostaining Antibodies and Reagents
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Anti-eNOS antibody was purchased from BD Biosciences (San Jose, CA, USA). Anti-SM22a antibody was obtained from Abcam (Cambridge, UK). Anti-actin antibody was purchased from MP Biomedicals (Aurora, OH, USA). NG2 antibody was obtained from Millipore Bioscience (Temecula, CA, USA). Anti-pH3 antibody was purchased from Cell Signaling Technology (Boston, MA, USA). GSL I-isolectin B4 was obtained from Vector Laboratories (Burlingame, CA, USA). Alexa Fluor 405- and 488-conjugated streptavidin, and Cy3-conjugated goat anti-rabbit secondary antibodies were purchased from Molecular Probes, Inc. (Carlsbad, CA, USA). IRDye700- and IRDye800-conjugated rabbit and mouse secondary antibodies were obtained from Li-COR Bioscience (Lincoln, NE, USA).
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