Immersol 518

Manufactured by Zeiss

Immersol 518 is a high-performance immersion oil designed for use with microscopy applications. It is formulated to have a refractive index of 1.518, which matches the refractive index of glass coverslips commonly used in microscopy. The oil is designed to provide optimal optical performance and clarity when used with Zeiss microscopes and objectives.

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Lab products found in correlation

Axiocam mrm, by Zeiss (1 mentions) Alexa fluor 488 streptavidin, by Jackson ImmunoResearch (1 mentions) Axiovision software, by Zeiss (1 mentions) Axiovision, by Zeiss (1 mentions) Slowfade gold, by Thermo Fisher Scientific (1 mentions) Alexa fluor 647 affinipure donkey anti goat, by Jackson ImmunoResearch (1 mentions) Q150r es, by Zeiss (1 mentions) Evo 15 esem, by Zeiss (1 mentions)

Spelling variants of this product

Immersol (8 citations) Immersol 518 F immersion oil (6 citations) Immersol 518 F immersion media (3 citations)

3 protocols using immersol 518

Large-Area Imaging of Scaffold Structures

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Tiled images of the entire scaffold were obtained using the 10× objective, were composed of either 2 × 2 tiles (927 µm × 927 µm) or 4 × 4 tiles (1,270 µm × 1,270 µm), depending on the scaffold architecture. These 10× magnification images required no immersion medium and were taken with an EC Plan-Neofluar N.A. = 0.3. In addition, single-frame and 2 × 2 tiled images (250 µm × 250 µm) were captured using a 40× objective for data analysis. The 40× magnification images were taken in Zeiss Immersol 518 immersion medium with refractive index n = 1.518 at 23 °C. Oversampling for all images was at least 2× as dictated by Nyquist sampling. Pinhole diameters for all images ranged from 1 to 2 airy unit (AU), with most measurements performed at ∼1.6 AU. A 20% tile overlap and online stitching permitted high-resolution large-area imaging of scaffold structures of interest. Confocal z stacks were reconstructed using ImageJ software.

Yan Z., Han M., Shi Y., Badea A., Yang Y., Kulkarni A., Hanson E., Kandel M.E., Wen X., Zhang F., Luo Y., Lin Q., Zhang H., Guo X., Huang Y., Nan K., Jia S., Oraham A.W., Mevis M.B., Lim J., Guo X., Gao M., Ryu W., Yu K.J., Nicolau B.G., Petronico A., Rubakhin S.S., Lou J., Ajayan P.M., Thornton K., Popescu G., Fang D., Sweedler J.V., Braun P.V., Zhang H., Nuzzo R.G., Huang Y., Zhang Y, & Rogers J.A. (2017). Three-dimensional mesostructures as high-temperature growth templates, electronic cellular scaffolds, and self-propelled microrobots. Proceedings of the National Academy of Sciences of the United States of America, 114(45), E9455-E9464.

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Visualizing the Nuclear Lamina by Immunofluorescence

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Cells were prepared for immunofluorescence by plating on sterilized 25-mm round coverslips (German borosilicate glass #1.5; Harvard Apparatus) in six-well tissue culture dishes. Immunofluorescence was carried out as previously described (12 (link), 114 (link)). The nuclear lamina was visualized using an anti-LMNB antibody (sc-6217, goat IgG; Santa Cruz Biotechnology, Inc.) and Alexa Fluor 647 AffiniPure Donkey Anti-Goat (#705-606-147; Jackson Immunoresearch) for secondary detection. Biotinylation was detected using streptavidin-488 (#016-540-084, Alexa Fluor 488 Streptavidin; Jackson Immunoresearch). Immunofluorescence samples were mounted in SlowFade gold (Life Technologies). All imaging was performed on an inverted fluorescence microscope (AxioVision; Carl Zeiss) fitted with an ApoTome and camera (AxioCam MRm; Carl Zeiss). The objective lens used was a 63× apochromat oil immersion (Carl Zeiss) with an NA of 1.5 (Immersol 518; Carl Zeiss). All immunofluorescence was performed at room temperature on #1.5 coverslips. AxioVision software (Carl Zeiss) was used for image acquisition. Images were exported as TIFFs to (FIJI ImageJ, National Institutes of Health) for further analyses (115 (link)).

Wong X., Cutler J.A., Hoskins V.E., Gordon M., Madugundu A.K., Pandey A, & Reddy K.L. (2021). Mapping the micro-proteome of the nuclear lamina and lamina-associated domains. Life Science Alliance, 4(5), e202000774.

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Spectroscopic Characterization of Fruit Surfaces

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We took macroscale photographs using a Sony a300 DSLR and Sony SAL30M28 macrolens and x-rite ColorChecker for fruit image calibration (processed using MATLAB Image processing toolbox). For spectral measurement, we used a DH-2000-BAL OceanOptics lamp, QR400-7-UV-Vis double-ended fiber, and an Avantes Flame UV-Vis Spectrometer. We calibrated it on an Avantes white diffuser standard. We took reflectance measurements using a metal mount-block holding the fiber normal to the surface (tangent to the fruit surface in the case of round fruits) at 7-mm distance. Spectra shown in Fig. 2 are averages across samples, smoothed over λ = 7.5 nm. Immersion oil was Zeiss Immersol 518. We prepared samples for SEM by mounting them on carbon tape and coating them with 5-nm gold in a Quorum Q150RES sputter coater; then, we imaged them in Zeiss Evo 15 ESEM under vacuum (standard settings: 10 kV, 50 pA).

Middleton R., Tunstad S.A., Knapp A., Winters S., McCallum S, & Whitney H. (2024). Self-assembled, disordered structural color from fruit wax bloom. Science Advances, 10(6), eadk4219.

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