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Atrazine

Manufactured by AccuStandard
Sourced in United States

Atrazine is a chemical standard used for analytical testing and research purposes. It is a white crystalline solid typically used as a reference material for the identification and quantification of atrazine in various matrices. The core function of Atrazine is to serve as a calibration standard to ensure the accuracy and reliability of analytical methods.

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2 protocols using atrazine

1

Atrazine Degradation Pathway Elucidation

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Atrazine (99.9% purity) was purchased from AccuStandard, Inc., USA. HydroxyAtrazine (2-hydroxy-4-ethylamino-6-isopropylamino-1,3,5-s-triazine) (99.0% purity) and cyanuric acid (1,3,5-striazine-2,4,6-triol) (99.0% purity) were purchased from J &K Chemical Ltd, Beijing, China. Urea (99.9% purity) was purchased from National Institute of Metrology, Beijing, China.
Enrichment medium consisted of a mineral salts medium (MSM) and 100 mg·L−1 Atrazine as the sole nitrogen source, plus sucrose 3 g·L−1 as carbon source, and was autoclaved at 121°C for 30 minutes. The mineral salts medium (MSM) contained (per liter) 1.6 g of K2HPO4, 0.40 g of KH2PO4, 0.20 g of MgSO4·7H2O and 0.10 g of NaCl. Trace element solution contained (per liter): EDTA, 2.5 g; FeSO4·7H2O, 1.0 g; ZnSO4·7H2O, 5.0 g; MnSO4·H2O, 1.0 g; CuSO4·5H2O, 0.40 g; Na2B4O7·10 H2O, 0.20 g; Na2MOO4·2H2O, 0.25 g. The pH of the medium was adjusted to 7.0 with 1 M NaOH solution.
The isolated microorganisms were maintained in agar slants consisting of the MSM supplemented with 16 g of agar per liter complemented with cyanuric acid or Atrazine, 100 mg·L−1 plus sucrose, 3 g·L−1.
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2

Resin Effectiveness for Compound Fractionation

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The resin effectiveness test was designed to assess the feasibility of compound fractionation within the iTIED and determine the adsorption abilities of several resin types. For a successful in situ TIE, the iTIED would have to remove or significantly reduce the concentration of target compounds as the source water passes through the resin chambers.
A 21-L spiked solution was used as the source water. The volume of solution was determined based on the combined flow rates of the 17 iTIED chambers and the length of the experiment (2 h). Cadmium (Alfa Aesar), cupric chloride (Nanjing Chemical Reagent), lead nitrate (Nanjing Chemical Reagent), zinc sulfate heptahydrate (Sigma-Aldrich), BPA (Aldrich), atrazine (AccuStandard), pyrene (AccuStandard), and ammonium chloride (Sigma) were added to 21 L of Milli-Q water in 2-mg/L concentrations. The iTIED processed this mixture for 2 h.
Resins utilized in the first test were zeolite, NDA-88, NDA-150, TP-207, and activated carbon. There were 3 replicate treatments for each resin, with 5 g of each sorbent in the respective chambers, and 2 replicates for the no-resin positive control chambers. Flow rate was recorded using the outflow from the check valves. Because of variation between the pumps, some voltages were adjusted during the exposure to achieve minimal variation between
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