Enrichment medium consisted of a mineral salts medium (MSM) and 100 mg·L−1 Atrazine as the sole nitrogen source, plus sucrose 3 g·L−1 as carbon source, and was autoclaved at 121°C for 30 minutes. The mineral salts medium (MSM) contained (per liter) 1.6 g of K2HPO4, 0.40 g of KH2PO4, 0.20 g of MgSO4·7H2O and 0.10 g of NaCl. Trace element solution contained (per liter): EDTA, 2.5 g; FeSO4·7H2O, 1.0 g; ZnSO4·7H2O, 5.0 g; MnSO4·H2O, 1.0 g; CuSO4·5H2O, 0.40 g; Na2B4O7·10 H2O, 0.20 g; Na2MOO4·2H2O, 0.25 g. The pH of the medium was adjusted to 7.0 with 1 M NaOH solution.
The isolated microorganisms were maintained in agar slants consisting of the MSM supplemented with 16 g of agar per liter complemented with cyanuric acid or Atrazine, 100 mg·L−1 plus sucrose, 3 g·L−1.