Pdcd5 sirna2

The miR-766 mimics, miR-766 inhibitor and the corresponding negative controls (mimics NC and inhibitor NC) were purchased from Genepharma (Shanghai, China). The PDCD5 siRNA1, PDCD5 siRNA2 and siRNA Control were purchased from Invitrogen (USA). The target sequences were shown as follows: PDCD5 siRNA1: 5′-GAGAAUUACCUUAUACAGTT-3′; PDCD5 siRNA2: 5′-AGAAUUACCUUAUACAGA-3′; siRNA Control: 5′-UUCUCCGAACGUGUCACGUTT-3′. The above agents were separately transfected or co-transfected into A431 and SCL-1 cells by Lipofectamine^® 3000 Reagent (Invitrogen, USA). The grouping was shown as follows: Control group (no-treated group), NC mimics group (transfected with mimics NC), miR-766 mimics group (transfected with miR-766 mimics), NC inhibitor group (transfected with miR-766 inhibitor NC), miR-766 inhibitor group (transfected with miR-766 inhibitor), siRNA group (transfected with PDCD5 siRNA), siRNA NC group (transfected with siRNA Control), si-NC + miR-766 INC group (transfected with siRNA Control and miR-766 inhibitor NC), siRNA + miR-766 INC group (transfected with PDCD5 siRNA and miR-766 inhibitor NC), siRNA + miR-766 inhibitor group (transfected with PDCD5 siRNA and miR-766 inhibitor), and si-NC + miR-766 inhibitor group (transfected with siRNA Control and miR-766 inhibitor). After 48 h of transfection, cells were used in further assays.