Sn 38

Bafetinib, dynasore, fexofenadine, sirtinol, eprosartan mesylate and PRT062607 were purchased or provided as samples from Selleck Chemicals (Houston, USA). Dulbecco’s modified Eagle’s Medium (DMEM), fetal bovine serum (FBS), penicillin/streptomycin and trypsin 0.25% were purchased from Hyclone (GE Healthcare Life Science, Pittsburgh, PA). Bovine Serum Albumin (BSA), monoclonal antibody C219 (against ABCB1), monoclonal antibody BA3R (against beta-actin), Alexa Fluor 488 conjugated goat anti-mouse IgG secondary antibody, RNase A and SN-38 were purchased from Thermo Fisher Scientific Inc. (Rockford, IL). Monoclonal antibody BXP-21 (against BCRP) was purchased from GeneTex (Irvine, CA). HRP-labeled rabbit anti-mouse secondary IgG was purchased from Santa Cruz Biotechnology (Dallas, TX). Monoclonal antibody P7965 (against ABCB1), paclitaxel, doxorubicin, cisplatin, mitoxantrone, verapamil, 3-(4,5-dimethylthiazol-yl)-2,5-diphenyltetrazolium bromide (MTT), dimethylsulfoxide (DMSO), Triton X-100, propidium iodide and paraformaldehyde were products from Sigma-Aldrich (St. Louis, MO). [³H]-paclitaxel (15 Ci/mmol) and [³H]-mitoxantrone (2.5 Ci/mmol) were purchased from Moravek Biochemicals, Inc (Brea, CA). The chemicals used in ATPase assay not included in the kit were same as those in our previous study33 (link). Fumitremorgin C (FTC) was a gift from Dr. Susan Bates (NIH, Bethsda, MD).