3h gaba

All experiments involving animals were approved by and carried out in accordance with the guidelines of the Institutional Animal Care and Use Committee of the Federal University of Rio de Janeiro (permit number IBCCF-035), and all efforts were made to minimize suffering. Fertilized White Leghorn chicken eggs were obtained from a local hatchery. In this study, we used around 55 eggs (110 retinae). Embryos were staged according to [15 (link)] and sacrificed by decapitation on embryonic day 8 (E8). The eyes were removed and the retinas dissected out in a Ca²⁺- and Mg²⁺-free Hanks' (CMF) solution. We confirm that all animal use and experimental procedures were carried out in accordance with the guidelines of the Brazilian Society of Neuroscience and Behavior (SBNeC). Dulbecco's modified Eagle's medium (DMEM), fetal calf serum (FCS) and gentamycin were obtained from Gibco (USA). MK801 (Cat. No.0924) and DNQX were obtained from Tocris-Cookson (St. Louis, MO USA), ATP, propidium iodide, GABA, A-740003 (N-(1-{[(cyanoimino)(5-quinolinylamino) methyl] amino}-2,2-dimethylpropyl)-2-(3,4-dimethoxyphenyl)acetamide, Brilliant Blue G (BBG), thapsigargin, glutathione were from sigma. BAPTA-AM, CM-H2DCFDA and Fura-2 AM were obtained from Molecular Probes. [³H] GABA was from Amersham Pharmacia Biotech, Piscataway, NJ, USA. All other reagents were of analytical grade.