Differentiation capacity of hMSCs was performed using the Human Mesenchymal Stem Cell Function Identification Kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturers' protocol. After 20 days under differentiation conditions, fatty acid binding protein-4 (FABP-4) and osteocalcin for adipogenic and osteogenic differentiation were fluorescently labeled, respectively. Nuclei were counter stained with 4′,6-diamidino-2-phenylindol (DAPI, Invitrogen, Carlsbad, CA, USA). Samples were analyzed using ELYRA PS.1 LSM 780 microscope (Carl Zeiss, Jena, Germany) and ZEN2011 software (Carl Zeiss, Göttingen, Germany).
Elyra ps 1 lsm 780 microscope
Manufactured by Zeiss
Sourced in Germany
The ELYRA PS.1 LSM 780 microscope is a high-performance confocal laser scanning microscope designed for advanced imaging applications. It offers high-resolution optical sectioning and 3D imaging capabilities.
Automatically generated - may contain errors
Lab products found in correlation
4 6 diamidino 2 phenylindol dapi, by Thermo Fisher Scientific (1 mentions)
Zen 2011, by Zeiss (1 mentions)
Alexa fluor 647, by Abcam (1 mentions)
Triton x, by Merck Group (1 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions)
Dako mounting medium, by Agilent Technologies (1 mentions)
α mhc, by Abcam (1 mentions)
Cold water fish skin gelatin, by Merck Group (1 mentions)
Anti mouse alexa fluor 647, by Abcam (1 mentions)
Sp5 laser scanning confocal microscope, by Leica (1 mentions)
Saponin, by Merck Group (1 mentions)
Paraformaldehyde (pfa), by Merck Group (1 mentions)
2 protocols using elyra ps 1 lsm 780 microscope
1
Differentiation Capacity of hMSCs
2
Immunofluorescence Staining of Cardiomyocytes
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Cells on coverslips were fixed in 4% paraformaldehyde (Sigma) for 1 hr at 4°C, permeabilized with 0.1% Triton-X (Sigma), and blocked with 0.2% cold-water fish skin gelatin (Sigma)/10% FCS (GIBCO). All antibodies were incubated in blocking solution plus 0.01% saponin (Sigma), primary antibodies (TBX3, 1:200 [Abnova]; α-MHC, 1:200; [Abcam]; HCN4, 1:100 [Alomone Labs]; Connexin 30.2, 1:200 [Abcam]; and Connexin 45, 1:200 [Abcam]) for 1 hr at RT, secondary antibodies (anti-sheep Alexa Fluor 633, 1:500 [Life Technologies]; anti-mouse Alexa Fluor 647, 1:500 [Abcam]; and anti-rabbit Alexa Fluor 647, 1:500 [Abcam]) for 45 min at RT with additional phalloidin (1:500; Enzo Life Science) and bisBenzimide (DAPI, 1:2,000; Sigma). The coverslips were mounted with Dako mounting medium (DAKO) to glass slides and analyzed by fluorescence microscopy using a Leica SP-5 confocal laser scanning microscope (Leica Microsystems) or an ELYRA PS.1 LSM 780 microscope (Carl Zeiss).
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