Alexa fluor 594 goat anti guinea pig igg

Manufactured by Abcam

Sourced in United Kingdom

Alexa Fluor 594 Goat; Anti‐guinea Pig IgG is a secondary antibody conjugated with the Alexa Fluor 594 fluorescent dye. It is designed to detect and visualize guinea pig immunoglobulin G (IgG) in various applications, such as immunohistochemistry, flow cytometry, and western blotting.

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Lab products found in correlation

Alexa fluor 488 goat anti rabbit igg, by Proteintech (2 mentions) Alexa fluor 594 goat anti mouse igg, by Proteintech (2 mentions) Glucagon, by Santa Cruz Biotechnology (1 mentions) Somatostatin, by Santa Cruz Biotechnology (1 mentions) Ix51 microscope, by Olympus (1 mentions)

2 protocols using alexa fluor 594 goat anti guinea pig igg

Immunohistochemical Profiling of Pancreatic Tissues

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Pancreatic tissues were fixed with 4% paraformaldehyde and sliced (3–5 µm) after paraffin embedding. IHC was performed using the EnVision+System‐horse radish peroxidase (HRP) (DAB) kit (Dako, USA) for target antibody or control IgG staining. For IF staining, the primary antibody was treated at 4 °C overnight, and the secondary antibody was treated with Alexa Fluor 594 Goat; Anti‐guinea Pig IgG (#ab150188, Abcam, Cambridge, UK); Alexa Fluor 594 Goat Anti‐Mouse IgG (#SA00006‐3, Proteintech, Illinois, USA) and Alexa Fluor 488 Goat Anti‐Rabbit IgG (#SA00006‐2, Proteintech, Illinois, USA) at 37 °C for 1 h. Finally, the nuclei were stained with DAPI. Microscopic analyses were performed using an Olympus IX51 microscope (Tokyo, Japan) and ImageJ software.

Ding L., Sun Y., Liang Y., Zhang J., Fu Z., Ren C., Li P., Liu W., Xiao R., Wang H., Zhang Z., Yue X., Li C., Wu Z., Feng Y., Liang X., Ma C, & Gao L. (2024). Beta‐Cell Tipe1 Orchestrates Insulin Secretion and Cell Proliferation by Promoting Gαs/cAMP Signaling via USP5. Advanced Science, 11(16), 2304940.

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Immunostaining of Pancreatic Tissues

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Pancreatic tissues were fixed in 4% paraformaldehyde and embedded in paraffin. For IHC, 5-μm sections were stained with antibodies or control IgG using the EnVision+System-HRP (DAB) kit (Dako North America Inc, USA) according to the manufacturer’s instructions. For IF, tissues were incubated with antibodies against insulin (Abcam; Santa Cruz Biotechnology), glucagon (Santa Cruz Biotechnology), somatostatin (Santa Cruz Biotechnology), Zhx2 (Proteintech), PAX6 (Santa Cruz Biotechnology), and Ki67 (Abcam) overnight at 4°C and incubated with antibodies against Alexa Fluor 594 goat anti-guinea pig IgG (Abcam), Alexa Fluor 594 goat anti-mouse IgG (Proteintech), and Alexa Fluor 488 goat anti-rabbit IgG (Proteintech) for 1 h at 37°C. Microscopy analysis was performed with an Olympus IX51 microscope and ImageJ software.

Ding L., Zhang Y., Wang Y., Wang Y., Tong Z., Li P., Chen C., Wang B., Yue X., Li C., Wu Z., Liang X., Ma C, & Gao L. (2023). Zhx2 maintains islet β-cell mass and function by transcriptionally regulating Pax6. iScience, 26(6), 106871.

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