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Anti human calprotectin

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Anti-human Calprotectin is a laboratory reagent used for the detection and quantification of the Calprotectin protein in human samples. Calprotectin is a calcium-binding protein found in neutrophils and serves as a biomarker for various inflammatory conditions.

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Lab products found in correlation

Draq5, by Cell Signaling Technology (2 mentions) Vector unmasking solution, by Vector Laboratories (2 mentions)

2 protocols using anti human calprotectin

1

Immunohistochemical Analysis of NEC

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Gastrointestinal tissue samples from surgically treated prematurely born neonates with NEC (n=5) were compared using immunohistochemical techniques to gastrointestinal tissue samples processed in parallel from control patients operated on for indications other than NEC (n=6). Immunohistochemistry on paraffin sections followed established procedures, including de-waxing, rehydration, and high temperature antigen retrieval (Vector Unmasking Solution, Vector Laboratories, Burlingame, CA) prior to blocking. Primary antibodies utilized in this study were as follows: anti-human Calprotectin (LifeSpan Biosciences, 1:100 dilution); anti-human Neutrophil Elastase (Hycult, 1:100 dilution). An Alexa-568 goat anti-rabbit secondary antibody (Molecular Probes, 1:1000 dilution) was used. Prior to imaging via confocal microscopy, tissue sections were incubated with DRAQ5 (Cell Signaling, 1:1000 dilution) as a DNA counterstain. Confocal microscopy was accomplished as described previously for immunocytochemistry (26 (link), 33 (link)).
MacQueen B., Christensen R., Yost C., Lambert D., Baer V., Sheffield M., Gordon P., Cody M., Gerday E., Schlaberg R., Lowe J, & Shepherd J. (2016). Elevated fecal calprotectin levels during necrotizing enterocolitis are associated with activated neutrophils extruding neutrophil extracellular traps. Journal of perinatology : official journal of the California Perinatal Association, 36(10), 862-869.
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2

Immunohistochemical Analysis of NEC

Check if the same lab product or an alternative is used in the 5 most similar protocols
Gastrointestinal tissue samples from surgically treated prematurely born neonates with NEC (n=5) were compared using immunohistochemical techniques to gastrointestinal tissue samples processed in parallel from control patients operated on for indications other than NEC (n=6). Immunohistochemistry on paraffin sections followed established procedures, including de-waxing, rehydration, and high temperature antigen retrieval (Vector Unmasking Solution, Vector Laboratories, Burlingame, CA) prior to blocking. Primary antibodies utilized in this study were as follows: anti-human Calprotectin (LifeSpan Biosciences, 1:100 dilution); anti-human Neutrophil Elastase (Hycult, 1:100 dilution). An Alexa-568 goat anti-rabbit secondary antibody (Molecular Probes, 1:1000 dilution) was used. Prior to imaging via confocal microscopy, tissue sections were incubated with DRAQ5 (Cell Signaling, 1:1000 dilution) as a DNA counterstain. Confocal microscopy was accomplished as described previously for immunocytochemistry (26 (link), 33 (link)).
MacQueen B., Christensen R., Yost C., Lambert D., Baer V., Sheffield M., Gordon P., Cody M., Gerday E., Schlaberg R., Lowe J, & Shepherd J. (2016). Elevated fecal calprotectin levels during necrotizing enterocolitis are associated with activated neutrophils extruding neutrophil extracellular traps. Journal of perinatology : official journal of the California Perinatal Association, 36(10), 862-869.
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