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Hct116 colon adenocarcinoma cell line

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The HCT116 colon adenocarcinoma cell line is a human-derived adherent cell line isolated from a colorectal carcinoma. This cell line is commonly used in cancer research to study the biology and characteristics of colon cancer cells.

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Lab products found in correlation

Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium with l glutamine, by Lonza (1 mentions) Hct116, by American Type Culture Collection (1 mentions)

2 protocols using hct116 colon adenocarcinoma cell line

1

Culturing Human Carcinoma Cell Lines

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The H1299 non-small cell lung carcinoma cell line21 (link) and HeLa MR and HeLaS325 (link) human cervical carcinoma cell lines were laboratory stocks. The HCT116 colon adenocarcinoma cell line was purchased from ATCC (Manassas, VA, USA). The human colon cancer cell lines, DLD-1 and HT29,26 (link) were a gift from Dr S Oda (National Kyushu Cancer Center, Fukuoka, Japan). The HCT116+Chr3 cell line22 (link) was a gift from Dr CR Boland (Baylor University Medical Center, Dallas, TX, USA). Cells were cultured in Dulbecco's modified Eagle's medium (Invitrogen, Carlsbad, CA, USA) supplemented with 10% (v/v) heat-inactivated fetal bovine serum, 100 μg/ml streptomycin and 100 units/ml penicillin, at 37 °C in 5% (v/v) CO2.
Oka S., Leon J., Tsuchimoto D., Sakumi K, & Nakabeppu Y. (2014). MUTYH, an adenine DNA glycosylase, mediates p53 tumor suppression via PARP-dependent cell death. Oncogenesis, 3(10), e121-.
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2

Cultivation of Isogenic Colon Cancer Cell Lines

Cited 1 time
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HCT 116 colon adenocarcinoma cell line (p53+/+) were obtained from ATCC (Manassas, VA); HCT 116 p53−/− cells were kindly provided by Dr. Bert Vogelstein [10 (link), 11 (link)]. Cells were cultured in RPMI-1640 medium with L-glutamine (BioWhittaker Inc., Walkersville, MD), containing 10% heat-inactivated fetal bovine serum and 100 units of penicillin/mL and 100 µg/mL of streptomycin (Biofluids, BioSource, Rockville, MD) in an incubator with 95% air, 5% CO2, and 95% humidity at 37°C.
Christner S.M., Clausen D.M., Beumer J.H., Parise R.A., Guo J., Huang Y., Dömling A.S, & Eiseman J.L. (2015). In vitro cytotoxicity and in vivo efficacy, pharmacokinetics and metabolism of pyrazole-based small-molecule inhibitors of Mdm2/4-p53 interaction. Cancer chemotherapy and pharmacology, 76(2), 287-299.
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