Organoid growth medium human

Manufactured by STEMCELL

Sourced in Canada

Organoid Growth Medium (human) is a specialized culture medium designed to support the growth and maintenance of human organoids. The medium provides the essential nutrients and growth factors required for the proliferation and differentiation of organoid cultures derived from human cells.

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Lab products found in correlation

Matrigel, by R&D Systems (2 mentions) Matrigel, by STEMCELL (1 mentions)

Close spelling variants of this product

Human IntestiCult Organoid Growth Medium Organoid Growth Medium

2 protocols using organoid growth medium human

Culturing Human Gastric Cancer Organoids

Cited 1 time

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The human organoid culture was performed as our previously reported [28 (link)]. Sterile gastric cancer tissues were cut into small pieces and digested with collagenase A. Then, the Matrigel (R&D Systems, USA) supplemented with growth factors was used to resuspend cells. After that, Organoid Growth Medium (human) (StemCell Technologies, Canada) was used to culture the mixtures that were seeded in a 24-well plate. Photographs of human GC organoids were taken daily by microscope.

Li Z., Fan H., Cao J., Sun G., Sen W.a.n.g., Lv J., Xuan Z., Xia Y., Wang L., Zhang D., Xu H, & Xu Z. (2021). Natriuretic peptide receptor a promotes gastric malignancy through angiogenesis process. Cell Death & Disease, 12(11), 968.

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Establishing Human Gastric Cancer Organoids

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Sterile gastric cancer tissues obtained from patients after gastric surgery were cut into small pieces and digested with collagenase A at room temperature for 40 min. Then, cells were resuspended in Matrigel (R&D Systems, Minneapolis, MN, USA). Fifty microliters of Matrigel containing cells with growth factors was seeded in a 24-well plate and supplemented with 500 μl of Organoid Growth Medium (human) (StemCell Technologies, Vancouver, Canada) for organoid growth. Photographs of human GC organoids were taken daily by microscopy.

Xia Y., Lv J., Jiang T., Li B., Li Y., He Z., Xuan Z., Sun G., Wang S., Li Z., Wang W., Wang L, & Xu Z. (2021). CircFAM73A promotes the cancer stem cell-like properties of gastric cancer through the miR-490-3p/HMGA2 positive feedback loop and HNRNPK-mediated β-catenin stabilization. Journal of Experimental & Clinical Cancer Research : CR, 40, 103.

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