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7 aca

Manufactured by Merck Group
Sourced in Germany

7-ACA is a chemical compound used as an intermediate in the production of pharmaceuticals. It serves as a precursor for the synthesis of various medications. The core function of 7-ACA is to provide a starting material for further chemical transformations and the development of drug products.

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2 protocols using 7 aca

1

Kinetic Study of Ester Hydrolysis Enzymes

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p-nitrophenol (p-NP) acetate (C2), butyrate (C4), caproate (C6), cefotaxime acid, tert-butyl acetate, 7-ACA, glyceryl tributyrate, cephalothin sodium, glyceryl trioleate, cefuroxime acid, and terpinyl and linalyl acetates were obtained from Sigma-Aldrich (Merck, Germany) or TCI (Tokyo, Japan). Qiagen (Hilden, Germany) provided Ni–NTA agarose. The K-ACET kit was obtained from Megazyme (Dublin, Ireland). The Fast Mutagenesis System, pACYCDuet-1 plasmid, and One-Step Cloning Kit were obtained from TransGen Biotech (Beijing, China), Miaoling Bioscience and Technology (Wuhan, China), and Vazyme Biotech (Nanjing, China), respectively. Synthesis of primers and estSJ gene was conducted by Sangon Biotech (Shanghai, China).
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2

Characterization of PGM-drug Interactions

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Mucin from porcine stomach (PGM type III, bound sialic acid 0.5-1.5%, partially purified powder), aztreonam, CFTR(inh)-172, ampicillin, tobramycin, levofloxacin, 7-ACA and rifampicin were purchased from Sigma Aldrich. Commercial ceftazidime for injection was supplied from Fresenius Kabi. PGM and drugs stock solutions for UV and fluorescence analysis were prepared in PBS (phosphate buffer solution 2 mM, pH = 7.4). Dissolution of the powder compounds was enhanced by sonication in an ultrasonic bath. The CFTR(inh)-172 stock solution was prepared by previously dissolution of the powder into a minimum volume of EtOH. All chemicals were of analytical reagent grade and were used without further purification. Millipore grade water was obtained from an in-house Millipore system (resistivity: 18.2 M cm at 25 °C).
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