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Vacutainer k3e plus blood collection tubes

Manufactured by BD
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Sourced in United Kingdom

The BD Vacutainer® K3E Plus blood collection tubes are designed for the collection and transportation of blood samples. These tubes contain the anticoagulant K3EDTA, which helps prevent blood clotting during the collection process.

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Lab products found in correlation

Falcon 50 ml conical tubes, by BD (1 mentions) Qiaamp dna mini extraction kit, by Qiagen (1 mentions) Falcon conical tube, by BD (1 mentions) Qiaamp dna mini kit, by Qiagen (1 mentions)

2 protocols using vacutainer k3e plus blood collection tubes

1

DNA Extraction from Blood and Saliva

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Blood samples (3 mL) were collected in BD Vacutainer® K3E Plus blood collection tubes and saliva samples in BD Falcon™ 50 mL conical tubes (BD, Plymouth, UK). DNA was extracted using the QIAamp DNA Mini extraction kit (Qiagen GmbH, Hilden, Germany), according to the manufacturer’s instructions for purification of DNA from blood or saliva, and stored at −40 °C. The concentration and purity of the DNA were measured using the NanoDrop 2000™ UV spectrophotometer with 280/260 and 280/230 absorbance ratios. The DNA samples, isolated from blood or saliva, were preserved in the Biobank of the Hospital Universitario Virgen de las Nieves, part of the Andalusian Public Health Service’s Biobank.
Pineda Lancheros L.E., Rojo Tolosa S., Gálvez Navas J.M., Martínez Martínez F., Sánchez Martín A., Jiménez Morales A, & Pérez Ramírez C. (2022). Effect of Single Nucleotide Polymorphisms in the Vitamin D Metabolic Pathway on Susceptibility to Non-Small-Cell Lung Cancer. Nutrients, 14(21), 4668.
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2

Nucleic Acid Isolation from Blood and Saliva

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Blood samples (3 ml) were collected in BD Vacutainer® K3E Plus Blood Collection Tubes. Saliva samples were collected in 50 ml BD Falcon™ conical tubes (BD, Plymouth, UK). DNA isolation was performed using the QIAamp DNA Mini Kit (QiagenGmBH, Hilden, Germany) according to the manufacturer's instructions for DNA purification from blood or saliva and stored at -40ºC.
Pérez-Ramírez C., Cañadas-Garre M., Alnatsha A., Villar E., Valdivia-Bautista J., Faus-Dáder M.J, & Calleja-Hernández M.Á. (2019). Pharmacogenetics of platinum-based chemotherapy: impact of DNA repair and folate metabolism gene polymorphisms on prognosis of non-small cell lung cancer patients. The pharmacogenomics journal, 19(2).
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