RNA was isolated with TRIzol, treated with DNase I, and digested and cleaned using R1014 RNA Clean & Concentrator-5 kit (Zymo Research). The cDNA was synthesized using qScript Flex cDNA kit (95049 Quanta) with oligo dT, and real-time quantitative polymerase chain reaction (qPCR) carried out using PerfeCTa SYBR Green FastMix (Quanta). Amplicon levels were quantified using standard curves and normalized to levels of Rplp0, all as previously reported [29 (link)]. Primers are listed in Table 1 .
R1014 rna clean concentrator 5 kit
Manufactured by Zymo Research
The R1014 RNA Clean & Concentrator-5 kit is a laboratory product designed to purify and concentrate RNA samples. The kit utilizes silica-based spin columns to remove contaminants and concentrate RNA for downstream applications.
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Lab products found in correlation
2 protocols using r1014 rna clean concentrator 5 kit
1
RNA Isolation, cDNA Synthesis, and qPCR Analysis
2
RNA Isolation, cDNA Synthesis, and qPCR Analysis
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RNA was isolated with TRIzol, treated with DNase I, and digested and cleaned using R1014 RNA Clean & Concentrator-5 kit (Zymo Research). The cDNA was synthesized using qScript Flex cDNA kit (95049 Quanta) with oligo dT, and real-time quantitative polymerase chain reaction (qPCR) carried out using PerfeCTa SYBR Green FastMix (Quanta). Amplicon levels were quantified using standard curves and normalized to levels of Rplp0, all as previously reported [29 (link)]. Primers are listed in Table 1 .
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