Algae cell concentrations were calculated by pipetting a 20 μL sample of each culture onto a Cellometer counting chamber (Nexcelom Bioscience, Lawrence, MA, USA) and analyzed using a Cellometer Auto X4 Cell Counter (Nexcelom Bioscience, Lawrence, MA, USA).
Cellometer counting chamber
Manufactured by Revvity
Sourced in United States
The Cellometer counting chamber is a specialized lab equipment designed for automated cell counting. It provides a consistent and reliable method for enumerating cells in a sample. The core function of the Cellometer counting chamber is to enable accurate and reproducible cell quantification.
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Lab products found in correlation
4 protocols using cellometer counting chamber
1
Algae Cell Concentration Quantification
2
Measuring Spermatozoa Viability in Queen Bees
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The spermatheca was ruptured with a pair of ridged forceps and the released contents mixed, transferred into a 1.7 ml centrifuge vial, and vortexed gently for 15 s. An aliquot of 200 µl was transferred to a 1.0 ml amber chromatography vial containing 2.0 µl propidium iodide solution and Sybr 14 dye from a Thermo-Fisher Live-Dead Sperm viability kit essentially as previously described56 (link). This aliquot was vortexed to mix and capped, allowing it to incubate at room temperature until all queens were processed, which took approximately 2.5 h.
After the spermathecal contents had incubated with the dyes for at least 10 min and no more than 3 h, 20 µl was transferred to a Nexcelom Cellometer counting chamber for spermatozoa count and viability imaging essentially as previously described11 (link),56 (link). The average of the three reads was taken for the measures of total spermatozoa concentration, and the viability was recorded as the ratio of live to total spermatozoa.
After the spermathecal contents had incubated with the dyes for at least 10 min and no more than 3 h, 20 µl was transferred to a Nexcelom Cellometer counting chamber for spermatozoa count and viability imaging essentially as previously described11 (link),56 (link). The average of the three reads was taken for the measures of total spermatozoa concentration, and the viability was recorded as the ratio of live to total spermatozoa.
3
Spermatozoa Viability Quantification
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The spermatheca was ruptured with a pair of ridged forceps and the released contents mixed, transferred into a 1.7 ml centrifuge vial, and vortexed gently for 15 s. An aliquot of 200 µl was transferred to a 1.0 ml amber chromatography vial containing 2.0 µl propidium iodide solution and Sybr 14 dye from a Thermo-Fisher Live-Dead Sperm viability kit essentially as previously described [48] . This aliquot was vortexed to mix and capped, allowing it to incubate at room temperature until all queens were processed, which took approximately 2.5 h.
After the spermathecal contents had incubated with the dyes for at least 10 min and no more than 3 h, 20 µl was transferred to a Nexcelom Cellometer counting chamber for spermatozoa count and viability imaging essentially as previously described [11, (link)48] . The average of the three reads was taken for the measures of total spermatozoa concentration, and the viability was recorded as the ratio of live to total spermatozoa.
After the spermathecal contents had incubated with the dyes for at least 10 min and no more than 3 h, 20 µl was transferred to a Nexcelom Cellometer counting chamber for spermatozoa count and viability imaging essentially as previously described [11, (link)48] . The average of the three reads was taken for the measures of total spermatozoa concentration, and the viability was recorded as the ratio of live to total spermatozoa.
4
Cultivation and Enumeration of B. burgdorferi and E. coli
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