Pro par xylene substitute

Manufactured by Anatech

Pro-Par is a xylene substitute designed for use in histology and pathology laboratories. It is a clear, colorless liquid with a mild odor. Pro-Par is intended to be used as a clearing agent and de-waxing solution in tissue processing and staining procedures.

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Lab products found in correlation

Automated stainer, by Leica (2 mentions) Spectra hemalast, by Leica (2 mentions) Axio scan z1, by Zeiss (1 mentions)

3 protocols using pro par xylene substitute

Histological Preparation and Imaging

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After imaging of the thick sections, tissue was dehydrated through graded alcohols and Pro-Par xylene substitute (Anatech, Battle Creek MI), and infiltrated with Paraplast wax medium (Leica Microsystems, Buffalo Grove IL). Tissue was embedded in EM-400 wax (Leica Microsystems, Buffalo Grove IL) and sectioned at 5 μm for hematoxylin and eosin (H&E) staining. Stained sections were digitally scanned at 10x (Zeiss Axio Scan Z1, Carl Zeiss Microscopy GmbH, Jena, Germany).

Tang Q., Lombardi A.F., Le N., Wong J.H., Williams J.L., Du J, & Chang E.Y. (2021). Novel fluorescent staining protocol for thick sections of human osteochondral tissues to facilitate correlation with MRI and CT. Skeletal radiology, 50(11), 2281-2288.

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Automated Histological Staining Procedure

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The staining procedure was run on a Leica automated stainer. Slides were heated to 65°C for 9 minutes, then cleared [with Pro-Par xylene substitute (Anatech Ltd)], and rehydrated before treatment with SPECTRA Hemalast (Leica) and sequential staining with hematoxylin for 4 minutes, bluing for 30 seconds, and eosin for 1 minute following a 1-minute 80% ethyl alcohol wash. Slides were then dehydrated, recleared with Pro-Par, and cover slipped using Permount.

Boudreau C.E., Najem H., Ott M., Horbinski C., Fang D., DeRay C.M., Levine J.M., Curran M.A, & Heimberger A.B. (2021). Intratumoral Delivery of STING Agonist Results in Clinical Responses in Canine Glioblastoma. Clinical Cancer Research, 27(20), 5528-5535.

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Automated Histological Staining Procedure

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The staining procedure was run on a Leica automated stainer. Slides were heated to 65degC for 9 minutes, then cleared (with Pro-Par xylene substitute (Anatech Ltd)), and rehydrated the slides before treatment with SPECTRA Hemalast (Leica) and sequential staining with hematoxylin for 4 min, bluing for 30 sec, and eosin for 1 min following a 1 min 80% ethyl alcohol wash. Slides were then dehydrated, re-cleared with Pro- Par, and cover slipped using Permount.

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