Microvettes cb 300z

Manufactured by Sarstedt

Sourced in United States

Microvettes (CB 300Z) are small sample collection tubes designed for the collection and storage of small volumes of blood or other biological fluids. These tubes have a capacity of 300 μL and are available with a range of additives, such as EDTA or lithium heparin, to prepare the sample for further analysis.

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Lab products found in correlation

Polypropylene tube, by Sarstedt (1 mentions) Nano pure water purifier, by Thermo Fisher Scientific (1 mentions) Xylazine rompun, by Bayer (1 mentions) μquant microplate reader, by Agilent Technologies (1 mentions) Mak126, by Merck Group (1 mentions) Mouse total bile acids assay kit, by Crystal Chem (1 mentions)

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Microvettes (19 citations)

2 protocols using microvettes cb 300z

High-Fat Diet Formulation and Analysis

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The formulated high-fat diets were produced by Dr. Forrest Nielsen (USDA Grand Forks Human Nutrition Research Center, Grand Forks, North Dakota). Monomethylsilanetriol (115-μg silicon/mL) was from LLR-G5 Ltd. (Castlebar, Ireland). Sterile, low-silicon-containing deionized drinking water was from Sigma-Aldrich Co. For sample analysis, ultra-high purity water was 18 mol/LΩ/cm, from a Branstead Nano-Pure water purifier (Thermo Scientific). Ketamine HCL (Ketasol-100) was from Dr. E Graeub AG, and xylazine (Rompun) was from Bayer HealthCare AG. Polypropylene tubes (13 mL and 25 mL) and microvettes (CB 300Z) were from Sarstedt Ltd. Eppendorf tubes (1.5 mL) were from Eppendorf. PBS was from PAA Laboratories GmbH.

Jugdaohsingh R., Kessler K., Messner B., Stoiber M., Pedro L.D., Schima H., Laufer G., Powell J.J, & Bernhard D. (2015). Dietary Silicon Deficiency Does Not Exacerbate Diet-Induced Fatty Lesions in Female ApoE Knockout Mice. The Journal of Nutrition, 145(7), 1498-1506.

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Blood Biomarker Quantification in Mice

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Mice were anesthetized using a ketamine-xylazine cocktail, and blood was retrieved by cardiac puncture into Microvettes (CB 300 Z, Sarstedt, Newton, NC, USA), which were centrifuged at 1000 × g for 10 min to obtain serum. Total bilirubin and total bile acid levels were determined using a bilirubin assay kit (MAK126, Sigma-Aldrich) and a Mouse Total Bile Acids Assay Kit (80471, Crystal Chem, Elk Grove Village, IL, USA), with a μQuant microplate reader (BioTek Instruments, Winooski, VT, USA).

Wang W., Pottorf T.S., Wang H.H., Dong R., Kavanaugh M.A., Cornelius J.T., Dennis K.L., Apte U., Pritchard M.T., Sharma M, & Tran P.V. (2021). IFT-A deficiency in juvenile mice impairs biliary development and exacerbates ADPKD liver disease. The Journal of pathology, 254(3), 289-302.

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