PITC and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich; Merck KGaA. N-acetyl-L-cysteine (NAC) was purchased from Beyotime Institute of Biotechnology. PITC was dissolved in DMSO to prepare stock solutions for in vitro assays and stored at −20°C. Cells in the control groups were treated with vehicle at an equal volume. To minimize toxicity, DMSO concentration was >0.1% for cell culture. Anti-poly-ADP-ribose polymerase (PARP; catalog no. 9542; 1:1,000), anti-cleaved caspase-3 (catalog no. 9662; 1:1,000), anti-cleaved caspase-9 (catalog no. 9502; 1:1,000), anti-GAPDH (catalog no. 5174; 1:1,000), anti-cytochrome c (Cyt c; catalog no. 4272; 1:1,000), anti-p53 (catalog no. 2527; 1:1,000) and anti-phosphorylated p53 (p-p53; catalog no. 2521; 1:1,000) antibodies were purchased from Cell Signaling Technology, Inc. Anti-cyclin A1 antibody (catalog no. ab118897; 1:1,000) was obtained from Abcam, Inc. Antibodies against Bcl-2 (catalog no. A2845; 1:1,000), Bax (catalog no. A0207; 1:1,000) and β-tubulin (catalog no. AC008; 1:1,000 for western blot analysis and 1:400 for immunofluorescence assay) were purchased from ABclonal Biotech Co., Ltd.
Anti phosphorylated p53 p p53
Manufactured by Cell Signaling Technology
The anti-phosphorylated p53 (p-p53) antibody is a laboratory tool used to detect the phosphorylated form of the p53 protein. P53 is a key regulator of cellular processes, and its phosphorylation plays a crucial role in its activation and function. This antibody can be used in various immunoassays, such as Western blotting, immunoprecipitation, and immunohistochemistry, to identify and quantify the phosphorylated p53 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Anti cleaved caspase 9, by Cell Signaling Technology (1 mentions)
Anti p53, by Cell Signaling Technology (1 mentions)
Anti gapdh, by Cell Signaling Technology (1 mentions)
Anti cytochrome c cyt c, by Cell Signaling Technology (1 mentions)
Antibodies against bcl 2, by Abcam (1 mentions)
N acetyl l cysteine, by Beyotime (1 mentions)
β tubulin, by ABclonal (1 mentions)
Anti β actin, by Merck Group (1 mentions)
Horseradish peroxidase conjugated anti rabbit igg, by Merck Group (1 mentions)
Anti total p44 42 mapk, by Cell Signaling Technology (1 mentions)
Anti total p53, by Cell Signaling Technology (1 mentions)
Anti total p38 mapk, by Cell Signaling Technology (1 mentions)
2 protocols using anti phosphorylated p53 p p53
1
Reagents and Antibodies for Cell Experiments
2
Western Blot Antibody Detection Protocol
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Western immunoblots were run as described previously [15] (link). Primary antibodies and their sources were as follows. Anti-total p38 MAPK, anti-total p53, anti-total p44/42 MAPK, anti-phosphorylated p44/42 (pp44/42) MAPK, and anti-phosphorylated p53 (pp53) were from Cell Signaling Technology. Anti-phosphorylated p38 (pp38) MAPK was from Millipore. Anti-β-actin and the secondary antibodies horseradish peroxidase-conjugated anti-rabbit IgG and anti-rabbit IgG were from Sigma-Aldrich. Anti-goat IgG was from Santa Cruz Biotechnology.
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