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Fluorophore conjugated monoclonal antibodies

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Fluorophore-conjugated monoclonal antibodies are a type of laboratory reagent used for various research and diagnostic applications. They consist of monoclonal antibodies that have been chemically conjugated with fluorescent dye molecules, known as fluorophores. These conjugated antibodies can be used to detect and visualize specific target molecules or structures within biological samples when exposed to light of the appropriate wavelength, enabling various analytical techniques.

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Lab products found in correlation

Lsrii flow cytometer, by BD (3 mentions) Cd106, by Thermo Fisher Scientific (1 mentions) Innohep, by Leo pharma (1 mentions)

4 protocols using fluorophore conjugated monoclonal antibodies

1

Multiparameter Flow Cytometry of Grafts

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Fluorophore-conjugated monoclonal antibodies against mouse CD3, CD4, CD8, CD25, FoxP3, CD11c, and NK1.1 (1:100; BD Bioscience or eBioscience) were used in various combinations to stain cells extracted from grafts. For staining, 1×106 cells were used. The Fc receptor was blocked by CD16/32 antibody. Cells were fixed and permeabilized using BD Cytofix/Cytoperm™ Fixation and intra-cellular staining was performed. Cells were assayed using a LSRII flow cytometer (BD Biosciences) and further analyzed with FlowJo software (Tree Star, Ashland, OR, USA).
de Almeida P.E., Meyer E.H., Kooreman N.G., Diecke S., Dey D., Sanchez-Freire V., Hu S., Ebert A., Odegaard J., Mordwinkin N., Brouwer T.P., Lo D., Montoro D., Longaker M.T., Negrin R.S, & Wu J.C. (2014). Transplanted Terminally Differentiated Induced Pluripotent Stem Cells Are Accepted By Immune Mechanisms Similar To Self-Tolerance. Nature communications, 5, 3903.
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2

Tissue Culture Immunophenotyping Protocol

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All tissue culture reagents and buffers were purchased from Gibco Laboratories, USA. Innohep was purchased from LEO Pharma, Denmark. All the fluorophore-conjugated monoclonal antibodies were purchased from BD Biosciences, USA, except for CD106 which was purchased from Invitrogen, Canada.
Sober S.A., Darmani H., Alhattab D, & Awidi A. (2021). Flow cytometric characterization of cell surface markers to differentiate between fibroblasts and mesenchymal stem cells of different origin. Archives of Medical Science : AMS, 19(5), 1487-1496.
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3

Multiparameter Flow Cytometry of Grafts

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Fluorophore-conjugated monoclonal antibodies against mouse CD3, CD4, CD8, CD25, FoxP3, CD11c, and NK1.1 (1:100; BD Bioscience or eBioscience) were used in various combinations to stain cells extracted from grafts. For staining, 1×106 cells were used. The Fc receptor was blocked by CD16/32 antibody. Cells were fixed and permeabilized using BD Cytofix/Cytoperm™ Fixation and intra-cellular staining was performed. Cells were assayed using a LSRII flow cytometer (BD Biosciences) and further analyzed with FlowJo software (Tree Star, Ashland, OR, USA).
de Almeida P.E., Meyer E.H., Kooreman N.G., Diecke S., Dey D., Sanchez-Freire V., Hu S., Ebert A., Odegaard J., Mordwinkin N., Brouwer T.P., Lo D., Montoro D., Longaker M.T., Negrin R.S, & Wu J.C. (2014). Transplanted Terminally Differentiated Induced Pluripotent Stem Cells Are Accepted By Immune Mechanisms Similar To Self-Tolerance. Nature communications, 5, 3903.
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4

Aortic Immune Cell Polarization Analysis

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Flow cytometry analysis for markers of T-cell and macrophage polarization was performed in suprarenal aortic tissue at day 7 after AngII-infusion. Control mice were injected with saline instead of MC-GFP to avoid interference with flow cytometry, while untreated animals received neither AngII nor MC IL-10 treatment. Aortic tissue was digested with Liberase (27 WU/ml) and DNase I (0.1 %) in DMEM media. Cells were counted and prepared for flow cytometry and 1×106 cells were used for staining. For intra- and extra-cellular staining, cells were fixed and permeabilized using BD Cytofix/Cytoperm™ Fixation. Fluorophore-conjugated monoclonal antibodies against mouse CD3, CD4, CD8, CD25, CD45, FoxP3, GranzymeB, F4/80, IL10, MRC1, TNF-α and viablitiy (1:100; BD Bioscience, Biolegend, eBioscience) were used. Cells were assayed using a LSRII flow cytometer (BD Biosciences) and further analyzed with FlowJo software (Tree Star, Ashland, OR, USA).
Adam M., Kooreman N., Jagger A., Wagenhaeuser M.U., Mehrkens D., Wang Y., Kayama Y., Toyama K., Raaz U., Schellinger I.N., Maegdefessel L., Spin J.M., Hamming J.F., Quax P.H., Baldus S., Wu J.C, & Tsao P.S. (2018). Systemic upregulation of IL-10 using a non-immunogenic vector reduces growth and rate of dissecting abdominal aortic aneurysm. Arteriosclerosis, thrombosis, and vascular biology, 38(8), 1796-1805.
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