Log In Sign up
The largest database of trusted experimental protocols

Anti translin

Manufactured by Thermo Fisher Scientific
Visit Supplier

Anti-translin is a lab equipment product used in molecular biology research. It functions as an enzyme inhibitor, specifically targeting the translin protein, which is involved in cellular processes. The core function of Anti-translin is to modulate the activity of translin, enabling researchers to study its biological roles.

Automatically generated - may contain errors

Lab products found in correlation

Pierce bca protein assay kit, by Thermo Fisher Scientific (4 mentions) Anti tubulin, by Cell Signaling Technology (2 mentions) Anti sirt1, by Cell Signaling Technology (2 mentions) Minute total protein extraction kit for adipose tissues, by Invent Biotechnologies (2 mentions) Anti trax, by Thermo Fisher Scientific (2 mentions) Hsc70, by Thermo Fisher Scientific (2 mentions) Ripa buffer, by Cell Signaling Technology (2 mentions) Protease and phosphatase inhibitor, by Merck Group (2 mentions)

2 protocols using anti translin

1

Liver Protein Extraction and Immunoblotting

Check if the same lab product or an alternative is used in the 5 most similar protocols
Liver samples were rinsed with PBS and homogenized with RIPA buffer (Cell Signaling Technology, Danvers, MA) containing protease and phosphatase inhibitors (MilliporeSigma, Burlington, MA). Total protein was extracted from eWAT using the Minute Total Protein Extraction Kit for Adipose Tissues (Invent Biotechnologies, Plymouth, MN). Protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA). Equal amounts of total protein were separated by an SDS-PAGE gel, transferred to a PVDF membrane and immunoblotted with antibodies: Anti-SIRT1, anti-tubulin (#9475T and #2148, Cell Signaling Technology), anti-translin and anti-trax (generated in our laboratory, [13 (link)]) and HSC70 (#PA529221, Thermo Fisher Scientific). Blots were developed with the ECL system. Band intensities were quantified from digital images, by densitometry, using ImageJ.
Shah A.P., Johnson M.D., Fu X., Boersma G.J., Shah M., Wolfgang M.J., Tamashiro K.L, & Baraban J.M. (2019). Deletion of translin (Tsn) induces robust adiposity and hepatic steatosis without impairing glucose tolerance. International journal of obesity (2005), 44(1), 254-266.
+ Open protocol
+ Expand
2

Liver Protein Extraction and Immunoblotting

Check if the same lab product or an alternative is used in the 5 most similar protocols
Liver samples were rinsed with PBS and homogenized with RIPA buffer (Cell Signaling Technology, Danvers, MA) containing protease and phosphatase inhibitors (MilliporeSigma, Burlington, MA). Total protein was extracted from eWAT using the Minute Total Protein Extraction Kit for Adipose Tissues (Invent Biotechnologies, Plymouth, MN). Protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA). Equal amounts of total protein were separated by an SDS-PAGE gel, transferred to a PVDF membrane and immunoblotted with antibodies: Anti-SIRT1, anti-tubulin (#9475T and #2148, Cell Signaling Technology), anti-translin and anti-trax (generated in our laboratory, [13 (link)]) and HSC70 (#PA529221, Thermo Fisher Scientific). Blots were developed with the ECL system. Band intensities were quantified from digital images, by densitometry, using ImageJ.
Shah A.P., Johnson M.D., Fu X., Boersma G.J., Shah M., Wolfgang M.J., Tamashiro K.L, & Baraban J.M. (2019). Deletion of translin (Tsn) induces robust adiposity and hepatic steatosis without impairing glucose tolerance. International journal of obesity (2005), 44(1), 254-266.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!