An organized chip array including 162 non-metastatic NSCLC tissue samples and non-neoplastic lung tissue samples was purchased from Outdo Biotech (HlugA180Su02 and HLug-Squ150Sur-02, Shanghai, China; http://www.superchip.com.cn/ ). A total of 107 paired frozen paraffin NSCLC tissue samples and matched adjacent non-cancerous tissue samples were obtained from the North China University of Science and Technology Affiliated People’s Hospital; the tissues were collected between 2009 and 2013. In addition, the serum samples from 80 patients with NSCLC and 40 healthy controls were obtained from the North China University of Science and Technology Affiliated People’s Hospital; serum samples were stored at -80°C. For studies using human data, the study was approved by ethics committee of the North China University of Science and Technology Affiliated People’s Hospital. (approval number: RH-2017-006) and written informed consent was obtained from all participants.
Hlug squ150sur 02
Manufactured by Shanghai Outdo Biotech Co.
Sourced in China
The HLug-Squ150Sur-02 is a laboratory equipment manufactured by Shanghai Outdo Biotech Co. It is designed for a specific core function, but further details about its intended use are not available.
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Lab products found in correlation
3 protocols using hlug squ150sur 02
1
Comprehensive NSCLC Tissue and Serum Profiling
2
Lung Cancer Tissue Microarray Immunohistochemistry
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Two lung cancer tissue microarrays HLugA180Su03 and HLug‐Squ150Sur‐02 were purchased from Shanghai Outdo Biotech. HLugA180Su03 contained 88 carcinoma tissues and paired paracarcinoma tissues of patients who had been pathologically diagnosed with lung adenocarcinoma. HLug‐Squ150Sur‐02 contained 74 carcinoma tissues and paired paracarcinoma tissues of patients who had been pathologically diagnosed with lung squamous cell carcinoma. The basic clinical information of all patients is listed in Tables S2 and S3 . The IHC of UCK2 were carried out using anti‐UCK2 Ab (ab60222; Abcam, Cambridge, UK). The tissue sections were dewaxed and the endogenous peroxidase was blocked by 1% hydrogen peroxide. After incubation with primary Ab against UCK2 overnight at 4°C and being washed, tissue sections were treated with biotinylated secondary Ab for 1 hour at room temperature. Finally, tissue sections were reacted with 3,3‐diaminobenzidine and counterstained with hematoxylin. The total score (values 0‐12) of protein expression was calculated by multiplying the percentage of immunopositive areas (0, 0%‐10%; 1, 11%‐25%; 2, 26%‐50%; 3, 51%‐75%; and 4, >75%) and immunostaining intensity (0, negative; 1, weak; 2, moderate; and 3, strong). A score of 4 or more defined high expression; a score less than 3 defined low expression.
3
Lung Cancer Tissue Microarray Analysis
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A lung cancer tissue microarray (#HLug-Squ150Sur-02), comprising samples and data from 75 patients with lung cancer, was purchased from Shanghai Outdo Biotech Co. Ltd. (Shanghai, China). All patients had undergone surgery from 2004 to 2007.
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