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Ova257 264 siinfekl peptide

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OVA257–264 (SIINFEKL) peptide is a synthetic peptide with the sequence SIINFEKL. This peptide is commonly used in research applications as a model antigen for studying T-cell responses in mice.

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Lab products found in correlation

Apc conjugated anti mouse cd11c, by Thermo Fisher Scientific (2 mentions) Pe conjugated anti mouse siinfekl peptide bound to h 2kb, by Thermo Fisher Scientific (2 mentions) Endothall, by Merck Group (1 mentions) Sb202190, by Merck Group (1 mentions) Sb203580, by Merck Group (1 mentions) Dusp1 6 inhibitor bci, by Merck Group (1 mentions) Okadaic acid, by Merck Group (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Human flt3l, by Thermo Fisher Scientific (1 mentions) Murine gm csf, by Thermo Fisher Scientific (1 mentions) Endofit, by InvivoGen (1 mentions)

4 protocols using ova257 264 siinfekl peptide

1

Quantifying SIINFEKL-Presenting DCs in dLN

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To analyze OVA257–264 (SIINFEKL) peptide (Anaspec) presenting DCs, dLNs were isolated and digested on day 7 post vaccination. dLN cells were enumerated and stained with APC conjugated anti-mouse CD11c (eBioscience 17–0114) and PE conjugated anti-mouse SIINFEKL peptide bound to H-2Kb (eBioscience 12–5743) for 15 minutes on ice. Cells were washed and assessed by flow cytometry.
Kim J., Li W.A., Choi Y., Lewin S.A., Verbeke C.S., Dranoff G, & Mooney D.J. (2014). Injectable, spontaneously assembling inorganic scaffolds modulate immune cells in vivo and increase vaccine efficacy. Nature biotechnology, 33(1), 64-72.
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2

Quantifying SIINFEKL-Presenting DCs in dLN

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To analyze OVA257–264 (SIINFEKL) peptide (Anaspec) presenting DCs, dLNs were isolated and digested on day 7 post vaccination. dLN cells were enumerated and stained with APC conjugated anti-mouse CD11c (eBioscience 17–0114) and PE conjugated anti-mouse SIINFEKL peptide bound to H-2Kb (eBioscience 12–5743) for 15 minutes on ice. Cells were washed and assessed by flow cytometry.
Kim J., Li W.A., Choi Y., Lewin S.A., Verbeke C.S., Dranoff G, & Mooney D.J. (2014). Injectable, spontaneously assembling inorganic scaffolds modulate immune cells in vivo and increase vaccine efficacy. Nature biotechnology, 33(1), 64-72.
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3

Immunomodulation Mechanism Analysis

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p38 MAPK inhibitors SB202190 and SB203580 (Sigma-Aldrich, St. Louis, MO, USA), PP2A inhibitors Okadaic acid (Sodium Salt, EMD Millipore, Billerica, MA, USA) and Endothall (Sigma-Aldrich), DUSP1/6 Inhibitor (BCI, EMD Millipore); OVA (257–264)/SIINFEKL peptide (Anaspec, Fremont, CA, USA), recombinant endotoxin-free OVA protein (Invivogen, San Diego, CA, USA), Toll-like receptor (TLR) agonists LPS (O127:B8 E. coli strain, Sigma-Aldrich), and mouse IL-6 ELISA and mouse IL-1β ELISA sets (BD Pharmingen, San Diego, CA, USA). Other antibodies and protein standards were purchased from Biolegend including purified antimouse IL-12 (p70) antibody (C18.2), biotin antimouse IL-12/IL-23 p40 antibody (C17.8), recombinant mouse IL-12 (p70) (ELISA Std.), purified antimouse IFN-γ antibody (AN-18), biotin antimouse IFN-γ antibody (R4-6A2), recombinant mouse IFN-γ (ELISA Std.), purified antimouse IL-4 antibody (11B11), and biotin antimouse IL-4 antibody (BVD6-24G2).
Liang D., Tian L., You R., Halpert M.M., Konduri V., Baig Y.C., Paust S., Kim D., Kim S., Jia F., Huang S., Zhang X., Kheradmand F., Corry D.B., Gilbert B.E., Levitt J.M, & Decker W.K. (2018). AIMp1 Potentiates TH1 Polarization and Is Critical for Effective Antitumor and Antiviral Immunity. Frontiers in Immunology, 8, 1801.
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4

Mouse BMDC Maturation and Antigen Priming

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Murine GM-CSF and human Flt3-L (both from Peprotech, Rocky Hill, NJ) were used to derive BMDCs from mouse bone marrow. CpG-Oligodeoxynucleotide (ODN), polyinosinic-polycytidylic acid [Poly(I:C)], monophosphoryl lipid A (MPLA) (all from Invivogen, San Diego, CA), and lipopolysaccharides (LPS) (Sigma, St Louis, MO) were used as adjuvants to induce maturation of BMDCs. Ovalbumin (OVA, model protein antigen unless specified otherwise) (EndoFit, Invivogen), fluorescently (Alexa Fluor 555)-labeled OVA (Molecular Probes, Eugene, OR), or OVA (257–264) SIINFEKL peptide (AnaSpec, Fremont, CA) was used as a model antigen. Ultra-pure chitosan (MW <200 kDa, deacetylation > 75%, NovaMatrix, Sandvika, Norway) was used to prime the mouse vaginal tract.
Park J., Frizzell H., Zhang H., Cao S., Hughes S.M., Hladik F., Koelle D.M, & Woodrow K.A. (2020). Flt3-L ENHANCES TRANS-EPITHELIAL MIGRATION AND ANTIGEN PRESENTATION OF DENDRITIC CELLS ADOPTIVELY TRANSFERRED TO GENITAL MUCOSA. Journal of controlled release : official journal of the Controlled Release Society, 329, 782-793.
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