Optical camera

Manufactured by Optronics

The Optical Camera is a high-precision imaging device designed to capture detailed visuals with exceptional clarity and accuracy. It features advanced optics and a sensitive sensor to produce crisp, high-resolution images. The core function of the Optical Camera is to provide precise visual data for scientific and technical applications.

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Lab products found in correlation

Metamorph image analysis software, by Molecular Devices (2 mentions) Tyramide signal amplification kit, by Thermo Fisher Scientific (1 mentions) Ki67 antibody, by Abcam (1 mentions) Alexa fluor 488 igg secondary antibodies, by Thermo Fisher Scientific (1 mentions)

2 protocols using optical camera

Immunofluorescence Analysis of Mouse Brainstem

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Following anesthetization, mice were transcardially perfused with Ringer’s solution and 4% paraformaldehyde in phosphate buffer. Dissected tissues were postfixed overnight and processed for O.C.T. Compound embedding. Immunofluorescence was performed using appropriate antibodies (Supp. Table 1) and Tyramide Signal Amplification (TSA) kits (Life Technologies Corporation), followed by DAPI counterstaining. Images of the medulla region of the brainstem (Supp. Fig. 1) were subsequently acquired on a Nikon Eclipse TE300 fluorescence inverted microscope (Nikon, Tokyo, Japan) equipped with an optical camera (Optronics, Goleta, CA) and MetaMorph image analysis software (Molecular Devices, Dowingtown, PA).

Solga A.C., Pong W.W., Walker J., Wylie T., Magrini V., Apicelli A.J., Griffith M., Griffith O.L., Kohsaka S., Wu G.F., Brody D.L., Mardis E.R, & Gutmann D.H. (2014). RNA-sequencing reveals oligodendrocyte and neuronal transcripts in microglia relevant to central nervous system disease. Glia, 63(4), 531-548.

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Astrocyte Proliferation Assay via Ki67

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Astrocytes were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Following overnight incubation with Ki67 antibodies (Abcam, Cambridge, MA), visualization was performed following incubation with Alexa Fluor 488 IgG secondary antibodies (Invitrogen, Carlsbad, CA). Cells were counterstained with DAPI. For each independent culture, at least five distinct microscopic fields were analyzed on a Nikon Eclipse TE300 fluorescence inverted microscope (Nikon, Tokyo, Japan) equipped with an optical camera (Optronics, Goleta, CA) and MetaMorph image analysis software (Molecular Devices, Dowingtown, PA).

Solga A.C., Pong W.W., Kim K.Y., Cimino P.J., Toonen J.A., Walker J., Wylie T., Magrini V., Griffith M., Griffith O.L., Ly A., Ellisman M.H., Mardis E.R, & Gutmann D.H. (2015). RNA Sequencing of Tumor-Associated Microglia Reveals Ccl5 as a Stromal Chemokine Critical for Neurofibromatosis-1 Glioma Growth. Neoplasia (New York, N.Y.), 17(10), 776-788.

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