Vinblastine sulfate, propidium iodide, sulforhodamine
B (SRB),
mouse monoclonal anti-α tubulin IgG, mouse monoclonal anti-β
actin IgG, fluorescein isothiocyanate (FITC)-conjugated antimouse
IgG, Hoechst 33258, and bovine serum albumin (BSA) were purchased
from Sigma (St. Louis, MO, USA). Alexa flour 555 conjugated goat antirabbit,
alexa flour 594 conjugated goat antirabbit IgG, and fetal bovine serum
(FBS) were purchased from Molecular probes, Invitrogen (Eugene,
OR, USA). Rabbit polyclonal anti-PARP-1 IgG was purchased
from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Horseradish peroxidase conjugated horse antimouse IgG and rabbit monoclonal
anti-γ-H2AX IgG were purchased from Cell Signaling Technologies.
Horseradish peroxidase conjugated goat antirabbit IgG was purchased
from Biorad (USA). Supersignal
west picochemiluminescent substrate was purchased from
Thermofisher Scientific. All other reagents were of analytical grade
and were purchased from HiMedia (Mumbai, India) and Sigma.
B (SRB),
mouse monoclonal anti-α tubulin IgG, mouse monoclonal anti-β
actin IgG, fluorescein isothiocyanate (FITC)-conjugated antimouse
IgG, Hoechst 33258, and bovine serum albumin (BSA) were purchased
from Sigma (St. Louis, MO, USA). Alexa flour 555 conjugated goat antirabbit,
alexa flour 594 conjugated goat antirabbit IgG, and fetal bovine serum
(FBS) were purchased from Molecular probes, Invitrogen (Eugene,
OR, USA). Rabbit polyclonal anti-PARP-1 IgG was purchased
from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Horseradish peroxidase conjugated horse antimouse IgG and rabbit monoclonal
anti-γ-H2AX IgG were purchased from Cell Signaling Technologies.
Horseradish peroxidase conjugated goat antirabbit IgG was purchased
from Biorad (USA). Supersignal
west picochemiluminescent substrate was purchased from
Thermofisher Scientific. All other reagents were of analytical grade
and were purchased from HiMedia (Mumbai, India) and Sigma.