All studies employed the PC3 human PCa cell lines engineered to exhibit high (PIP) or low (flu) PSMA expression. Both cell lines, generously provided by Dr Warren Heston (Cleveland Clinic), were grown in RPMI 1640 growth media (Corning Cellgro, Manassas, VA) containing 10% fetal bovine serum (FBS) (Sigma-Aldrich, St. Louis, MO) and 1% penicillin-streptomycin (Corning Cellgro, Manassas, VA) and were maintained in a humidified incubator under 5% CO2 at 37 ○C.
Rpmi 1640 growth media
Manufactured by Corning
Sourced in United States
RPMI 1640 growth media is a cell culture medium commonly used to support the growth and maintenance of various cell types, including mammalian cells, in in vitro experiments. It provides a balanced salt solution and essential nutrients required for cell proliferation and survival.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Corning (2 mentions)
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Penicillin, by Corning (1 mentions)
Streptomycin, by Corning (1 mentions)
Rpmi 8226, by Corning (1 mentions)
Bmscs, by American Type Culture Collection (1 mentions)
Rpmi 8226 cells, by American Type Culture Collection (1 mentions)
Cisplatin, by Bio-Techne (1 mentions)
Mccoy s 5a modified medium, by Thermo Fisher Scientific (1 mentions)
Du145, by Corning (1 mentions)
Lncap, by Corning (1 mentions)
Skov3, by Thermo Fisher Scientific (1 mentions)
Azd0156, by AstraZeneca (1 mentions)
Brca2, by Horizon Discovery (1 mentions)
Carboplatin, by Bio-Techne (1 mentions)
Skov3, by American Type Culture Collection (1 mentions)
Lncap, by American Type Culture Collection (1 mentions)
Du145, by American Type Culture Collection (1 mentions)
Olaparib, by AstraZeneca (1 mentions)
4 protocols using rpmi 1640 growth media
1
PSMA Expression Modulates PCa Cell Lines
2
Cultivation of Multiple Myeloma Cell Lines
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Human MM cells MM.1R, MM. 1S, U266, RPMI 8226 cells, and BM stromal cells (BMSCs) were purchased from the American Type Culture Collection (ATCC) (Manassas, VA). Bortezomib resistant cells MM.1S BTZ-R and RPMI8226 BTZ-R were a generous gift from Dr. Nathan Dolloff, The Medical University of South Carolina, Charleston, SC, USA. MM.1R, MM. 1S, and RPMI 8226 cells were cultured in RPMI 1640 growth media (Cellgro, Manassas, VA) supplemented with heat-inactivated 10% Fetal Bovine Serum (Sigma, St. Louis, MO), 100 I.U./ml penicillin, and 100 μg/ml streptomycin (Cellgro, Manassas, VA). U266 cells were cultured in RPMI 1640 with 15% FBS. BMSCs were procured from ATCC and cultured in Dulbecco’s Modified Eagle Medium (DMEM) (ATCC) supplemented with heat-inactivated 10% Fetal Bovine Serum (Sigma), 100 I.U./ml penicillin, and 100 μg/ml streptomycin (Cellgro, Manassas, VA). All cells were cultured in a 37°C and 5% CO2 incubator.
3
Cell Line Characterization and Culture
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LNCAP (RRID:CVCL_0395), DU145 (NCI-DTP catalog no. DU-145, RRID:CVCL_0105), and SKOV3 (NCI-DTP catalog no. SKOV-3, RRID:CVCL_0532) cells were obtained from ATCC. The DLD1 wild-type (RRID:CVCL_0248) and BRCA2−/− (RRID:CVCL_HD57) cell lines were purchased from Horizon Discovery. Cell line identification (short tandem repeat typing) was validated using the CellCheck assay (IDEXX Bioanalytics). All cell lines were validated free of virus and Mycoplasma contamination using the MycoSEQ assay (Thermo Fisher Scientific) or STAT-Myco assay (IDEXX Bioanalytics). All cell lines were grown according to supplier instructions. LNCAP, DU145, and DLD1 were grown in RPMI1640 growth media (Corning 17-105-CV) supplemented with 10% FBS and 2 mmol/L glutamine. SKOV3 were grown in McCoy's 5A (Modified) Medium (Thermo Fisher Scientific 16600082). Olaparib and AZD0156 (ATM inhibitor, ATMi) were made by AstraZeneca, carboplatin and cisplatin were bought from Tocris Bioscience (catalog no. 2626 and 15663-27-1). Olaparib, ATMi, and carboplatin were all solubilized in DMSO at 10 mmol/L stock concentration. cisplatin was solubilized in an aqueous solution at 1.67 mmol/L.
4
Lentiviral Overexpression of Thyroid Hormone Receptor in Thyroid Cancer Cells
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Cells were cultured in RPMI 1640 growth media with L-glutamine (300 mg/L), sodium pyruvate and nonessential amino acids (1%) (Corning Inc, Corning, NY, USA), supplemented with 10% fetal bovine serum (Thermo Fisher Scientific, Waltham, MA, USA) and penicillin-streptomycin (200 IU/L) (Corning) at 37°C, 5% CO2, and 100% humidity. Lentivirally modified SW1736 cells were generated as described (15, 22) with either an empty vector (SW-EV) or to overexpress TRβ (SW-TRβ). SW-EV and SW-TRβ were grown in the above conditions with the addition of 2 μg/ml puromycin (Gold Bio, St Louis, MO, USA). SW1736 and KTC-2 were authenticated by the Vermont Integrative Genomics Resource at the University of Vermont (Burlington, Vermont) using short tandem repeat profiles and Promega GenePrint10 System (SW1736, May 2019; KTC-2, October 2019). 8505C, OCUT-2, and CUTC60 were authenticated by University of Colorado by short tandem repeat profiles (8505C, June 2013; OCUT-2, June 2018; CUTC60, November 2018).
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