After the brain tissue was embedded in paraffin, the part between the optic chiasm and mammillary body was resected in the rostro-caudal direction. The tissue was serially sectioned on a paraffin slicer, and sections that were approximately 1.50 mm from the bregma were obtained [25 (link), 26 (link)]. After dewaxing the slices, 3% H2O2 was used to block endogenous peroxidase activity, and 0.01 M citric acid was used to retrieve the antigens prior to antibody incubation. Then, the slices were incubated with primary antibodies overnight at 4 °C [27 , 28 (link)]. The sections were immunohistochemically labelled to identify IL-6 (Bioss, China, 1:100), Gp130 (Santa Cruz, America, 1:20), pSTAT3 (Bioss, China, 1:50), NMDA receptors (Bioss, China, 1:50), and GAD67 (Abcam, England, 1:2000) and then incubated with secondary antibodies (anti-mouse for Gp130 and GAD67; anti-rabbit for NMDA receptors, pSTAT3 and IL-6) for 20 min at room temperature. For each rat, the positive neurons within the bilateral borders of the PVN were manually counted in three consecutive sections, and the average value is reported.
Nmda receptors
Manufactured by Abcam
Sourced in United Kingdom
NMDA receptors are ionotropic glutamate receptors that are essential for various neurological processes. They are ligand-gated ion channels that allow the passage of cations, particularly calcium, upon binding of the neurotransmitter glutamate. NMDA receptors play a crucial role in synaptic plasticity, learning, and memory formation.
Automatically generated - may contain errors
Lab products found in correlation
Gp130, by Abcam (3 mentions)
Gp130, by Santa Cruz Biotechnology (3 mentions)
Nmda receptors, by Bioss Antibodies (3 mentions)
Pstat3, by Bioss Antibodies (3 mentions)
Gad67, by Abcam (3 mentions)
Interleukin 6 (il 6), by Bioss Antibodies (3 mentions)
Interleukin 6 (il 6), by Abcam (3 mentions)
P stat3, by Abcam (3 mentions)
3 protocols using nmda receptors
1
Immunohistochemical Analysis of PVN Markers
2
Immunohistochemical Analysis of PVN Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After the brain tissue was embedded in para n, the part between the optic chiasm and mammillary body was resected in the rostro-caudal direction. The tissue was serially sectioned on a para n slicer, and sections that were approximately 1.50 mm from the bregma were obtained [25, 26] . After dewaxing the slices, 3% H 2 O 2 was used to block endogenous peroxidase activity, and 0.01 M citric acid was used to retrieve the antigens prior to antibody incubation. Then, the slices were incubated with primary antibodies overnight at 4°C [27, 28] . The sections were immunohistochemically labelled to identify IL-6 (Bioss, China, 1:100), Gp130 (Santa Cruz, America, 1:20), pSTAT3 (Bioss, China, 1:50), NMDA receptors (Bioss, China, 1:50), and GAD67 (Abcam, England, 1:2000) and then incubated with secondary antibodies (anti-mouse for Gp130 and GAD67; anti-rabbit for NMDA receptors, pSTAT3 and IL-6) for 20 minutes at room temperature. For each rat, the positive neurons within the bilateral borders of the PVN were manually counted in three consecutive sections, and the average value is reported.
3
Immunohistochemical Analysis of PVN Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After the brain tissue was embedded in paraffin, the part between the optic chiasm and mammillary body was resected in the rostro-caudal direction. The tissue was serially sectioned on a paraffin slicer, and sections that were approximately 1.50 mm from the bregma were obtained [25, 26] . After dewaxing the slices, 3% H 2 O 2 was used to block endogenous peroxidase activity, and 0.01 M citric acid was used to retrieve the antigens prior to antibody incubation. Then, the slices were incubated with primary antibodies overnight at 4°C [27, 28] . The sections were immunohistochemically labelled to identify IL-6 (Bioss, China, 1:100), Gp130 (Santa Cruz, America, 1:20), pSTAT3 (Bioss, China, 1:50), NMDA receptors (Bioss, China, 1:50), and GAD67 (Abcam, England, 1:2000) and then incubated with secondary antibodies (anti-mouse for Gp130 and GAD67; anti-rabbit for NMDA receptors, pSTAT3 and IL-6) for 20 minutes at room temperature. For each rat, the positive neurons within the bilateral borders of the PVN were manually counted in three consecutive sections, and the average value is reported.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!