The total cell protein extracts were prepared as described elsewhere [44 (link)]. Polyvinylidene difluoride (PVDF) membrane was incubated with the primary antibody anti-cofilin (1:200), anti-fibronectin (1:400), anti-α-tubulin (1:500), anti-TRF1 (1:1000), anti-TRF2 (1:3000), anti-RRN3 (1:3000), anti-lamin B1 (1:1000), anti-DNMT2 (1:500), anti-p21 (1:100) or anti-β-actin (1:1000) (Abcam, Novus Biologicals, Santa Cruz Technology) and the secondary antibodies conjugated to HRP (1:80000, Sigma-Aldrich). The respective proteins were detected using a Clarity™ Western ECL Blotting Substrate (BioRad, Warsaw, Poland) and a G:BOX imaging system (Syngene, Cambridge, UK) according to the manufacturer's instructions. Densitometry measurements of the bands were performed using GelQuantNET software (http://biochemlabsolutions.com/GelQuantNET.html ). The data represent the relative density normalized to β-actin.
Anti trf2
Manufactured by Abcam
Anti-TRF2 is a laboratory reagent used for the detection and quantification of TRF2 (Telomeric Repeat Binding Factor 2), a protein involved in telomere maintenance. This product can be used in various experimental techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression and localization of TRF2 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti p21, by Abcam (1 mentions)
Anti cofilin, by Abcam (1 mentions)
Anti lamin b1, by Abcam (1 mentions)
Anti dnmt2, by Abcam (1 mentions)
G box imaging system, by Syngene (1 mentions)
Anti trf1, by Abcam (1 mentions)
Clarity western ecl blotting substrate, by Bio-Rad (1 mentions)
Anti fibronectin, by Abcam (1 mentions)
Anti β actin, by Abcam (1 mentions)
Anti α tubulin, by Abcam (1 mentions)
Goat anti rabbit igg, by Merck Group (1 mentions)
Alkaline phosphatase, by Merck Group (1 mentions)
2 protocols using anti trf2
1
Western Blot Protein Expression Analysis
2
Nuclear Envelope Fractionation and Western Blot
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The nuclei were washed with 5:1+PO4 solution twice and incubated in 5:1+PO4 solution containing 1 mM CaCl2 for 10 min. This treatment induced shrinkage of the nucleoplasm and allowed for easier separation of the nuclear envelope (NE) and nuclear contents (gel). All solutions contained 1 mM PMSF. The samples of NE and gels were dissolved in Laemmli sample buffer and used for SDS-PAGE and western blot. The primary antibodies (anti-TRF2 from Abcam and anti-udTRF2) were diluted 1:500, and secondary antibodies, i.e. goat anti-guinea pig or goat anti-rabbit IgG (whole molecule) conjugated to alkaline phosphatase (Sigma) were diluted 1:10,000. Protein isolation and antibody production protocols have been published [26 ,27 ].
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