Acetaminophen (purity ≥ 98%) and stable labeled Acetaminophen-D4 solution (100 μg/mL in methanol) were purchased from Sigma–Aldrich (St. Louis, Mo, USA). LC-MS grade methanol, HPLC-grade ethanol, and analytical-grade ammonium formate were purchased from Wako Pure Chemical Industries (Osaka, Japan). A total of 6 lots of frozen, pooled human blood serum were purchased from Cosmo Bio (Tokyo, Japan).
Hplc grade ethanol
Manufactured by Fujifilm
Sourced in Japan
HPLC-grade ethanol is a highly purified form of ethanol specifically formulated for use in high-performance liquid chromatography (HPLC) applications. It is a colorless, volatile liquid with a characteristic alcoholic odor. The high purity and low impurity levels of this product make it suitable for use as a mobile phase component or solvent in HPLC analysis and related techniques.
Automatically generated - may contain errors
2 protocols using hplc grade ethanol
1
Quantification of Acetaminophen in Serum
2
Iodine Speciation Analysis by HPLC
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Guaranteed reagent (GR)-grade sodium iodide (NaI), sodium iodate (NaIO3), and potassium bromide (KBr) were obtained from Merck (Darmstadt, Germany). Acetonitrile (HPLC grade), sodium dihydrogenphosphate (NaH2PO4, GR grade), nitric acid (for toxic metal analysis) and L(+)-ascorbic acid (GR grade) were obtained from Kanto Chemical (Tokyo, Japan). HPLCgrade ethanol and tetrabutylammonium dihydrogen phosphate (TBA) solution were obtained from Wako Pure Chemical Industries (Osaka, Japan). Ortho dianisidine (ODA) was obtained from Sigma-Aldrich (St. Louis, MO, USA). A tetramethylammonium hydroxide solution (TMAH, TAMAPURE-AA-100) was obtained from Tama Chemicals (Kawasaki, Japan).
Standard solutions (1000 mg-I L -1 ) of I -and IO3 -were prepared by the dissolution of NaI and NaIO3, respectively, in deionized water. Mixtures of standard solutions of I -and IO3 - were prepared from stock solutions before the analysis. The mobile phase of the HPLC system described below consisted of a solution of 20% (v/v) acetonitrile and 80% (v/v) deionized water with 50 mM NaH2PO4 and 10 mM TBA. The solution for the postcolumn reaction consisted of 10% (v/v) ethanol, 1 M HNO3, 100 mM KBr, and 2 mM ODA. A solution of 0.2 M ascorbic acid solution in deionized water was prepared before use.
Standard solutions (1000 mg-I L -1 ) of I -and IO3 -were prepared by the dissolution of NaI and NaIO3, respectively, in deionized water. Mixtures of standard solutions of I -and IO3 - were prepared from stock solutions before the analysis. The mobile phase of the HPLC system described below consisted of a solution of 20% (v/v) acetonitrile and 80% (v/v) deionized water with 50 mM NaH2PO4 and 10 mM TBA. The solution for the postcolumn reaction consisted of 10% (v/v) ethanol, 1 M HNO3, 100 mM KBr, and 2 mM ODA. A solution of 0.2 M ascorbic acid solution in deionized water was prepared before use.
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