The XPW, illustrated in Fig. 1(a) , consists of two Glan polarizers (GL10-B, Thorlabs), two convex lenses (focal length of 1 m) and a BaF2 crystal (thickness of 2 mm). In the experiment, the partially-reflected portion of the main beam, with a pulse energy of 2 mJ, was loosely focused onto the BaF2 crystal. With the proper settings of the elements involved, the high-intensity central portion of the pulse may pass through the second polarizer with a transmission of ~99%, while the low-intensity background is attenuated considerably with a residual transmission of ~10−6. The pulse energy after XPW was ~100 μJ, corresponding to an overall efficiency of ~5%. In principle, the improvement of pulse contrast by XPW follows cubic law in the ideal case. However, practically an improvement of ~106 is typical due to limited extinction ratio of the polarizers.
Gl10 b
Manufactured by Thorlabs
Sourced in United States
The GL10-B is a general-purpose laboratory power supply from Thorlabs. It provides a variable output voltage up to 30 V and a maximum current of 3 A. The power supply features a digital display for monitoring the output.
Automatically generated - may contain errors
Lab products found in correlation
Xlpln25xwmp2, by Olympus (1 mentions)
Pci 6110, by National Instruments (1 mentions)
Gvsm002, by Thorlabs (1 mentions)
Sr570, by Stanford Research Systems (1 mentions)
Te2000 u, by Nikon (1 mentions)
Pm100d, by Thorlabs (1 mentions)
Isoplane sct 320, by Teledyne (1 mentions)
Cvh100, by Thorlabs (1 mentions)
4 protocols using gl10 b
1
Pulse Cleaning via Cross-Polarized Wave
2
Two-Photon Microscopy Setup for In Vivo Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In vivo imaging was performed on a custom-built two-photon setup based on a Zeiss upright microscope (AxioExaminer Z1) equipped with a 25 × water immersion objective (NA 1.05, WD 2 mm, Olympus XLPLN25XWMP2). Femtosecond pulses from an ultrafast Ti:Sapphire laser (Newport, Tsunami) whose intensity was modulated using a half-wave plate (Thorlabs, AHWP05M) and a polarizer (Thorlabs, GL10-B) is used as light source. The excitation beam is raster scanned using a galvo scanning mirror (Thorlabs, GVSM002) before entering the microscope body and is focussed on the imaging plane using the objective lens. The fluorescence that is collected by the objective lens in an epi-illumination geometry is then separated using a dichroic before being detected by photomultiplier module (H7422, Hamamatsu Corporation, Japan).
A low noise current preamplifier (Stanford Research Systems, SR570) was used to amplify the photomultiplier tube photocurrent, which was further digitized using a data acquisition board (National Instruments, PCI-6110). ScanImage (r 3.8.1) software was used to interface instrument control and generation of galvometric scan command. Image acquisition was accomplished using a custom Matlab script interfaced with z-drive of the microscope. The digitized signal was analysed using Matlab, Origin and ImageJ for further analysis.
A low noise current preamplifier (Stanford Research Systems, SR570) was used to amplify the photomultiplier tube photocurrent, which was further digitized using a data acquisition board (National Instruments, PCI-6110). ScanImage (r 3.8.1) software was used to interface instrument control and generation of galvometric scan command. Image acquisition was accomplished using a custom Matlab script interfaced with z-drive of the microscope. The digitized signal was analysed using Matlab, Origin and ImageJ for further analysis.
3
Quantitative SHG Microscopy of Microtubules
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The SHG data were acquired using a custom-built microscope based on a Nikon TE2000-U (Fig. 6 B ; Kaneshiro et al., 2018 (link)
Preprint). The sample was illuminated by a mode-locked Ti/sapphire laser (Chameleon Vision II; Coherent, Inc.) with an 810-nm wavelength, 80-MHz repetition rate, and 200-fs pulse duration through a Glan-laser polarizing prism (GL10-B; Thorlabs), high-speed polarization controller (350-160 and 350-80; Conoptics, Inc.), and a 40× dry objective lens (NA 0.95, CFI Apo; Nikon). The emitted light was detected with a photon-counting photomultiplier tube module (H10680-210; Hamamatsu Photonics) through a 100× objective lens (NA 1.45, CFI Apo, oil; Nikon) and filters (FF01-680/SP and FF01-405/10-25; Semrock). The SHG intensity data on the incident polarization angle (θ) were fitted with the following theoretical function to obtain three fitting parameters α, χzzz, and χzxx: where α denotes the angle of average orientation of a microtubule bundle and χzzz and χzxx are two components of SHG susceptibility tensor (Psilodimitrakopoulos et al., 2013 (link); Kaneshiro et al., 2018 (link)
Preprint).
Preprint). The sample was illuminated by a mode-locked Ti/sapphire laser (Chameleon Vision II; Coherent, Inc.) with an 810-nm wavelength, 80-MHz repetition rate, and 200-fs pulse duration through a Glan-laser polarizing prism (GL10-B; Thorlabs), high-speed polarization controller (350-160 and 350-80; Conoptics, Inc.), and a 40× dry objective lens (NA 0.95, CFI Apo; Nikon). The emitted light was detected with a photon-counting photomultiplier tube module (H10680-210; Hamamatsu Photonics) through a 100× objective lens (NA 1.45, CFI Apo, oil; Nikon) and filters (FF01-680/SP and FF01-405/10-25; Semrock). The SHG intensity data on the incident polarization angle (θ) were fitted with the following theoretical function to obtain three fitting parameters α, χzzz, and χzxx: where α denotes the angle of average orientation of a microtubule bundle and χzzz and χzxx are two components of SHG susceptibility tensor (Psilodimitrakopoulos et al., 2013 (link); Kaneshiro et al., 2018 (link)
Preprint).
4
Upconversion Nanoparticle Emission Spectroscopy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Downconverted emission spectra were recorded using a spectrograph (Princeton Instruments, NJ, USA, Isoplane SCT320) equipped with a cooled IR camera (Princeton Instruments, NJ, USA). The UCNP samples were placed into the cuvette holder (Thorlabs Inc., Newton, MA, USA, CVH100) equipped with a 1050 nm long-pass edge filter (Thorlabs Inc., Newton, MA, USA, FELH1050) and an optical collimator and then connected to the spectrograph. Samples were excited using a Ti:sapphire 980 nm laser (Spectra-Physics, Santa Clara, CA, USA, Mai Tai) with a pulse width = 100 fs and repetition rate = 80 MHz. The laser output beam was expanded with a 5× beam expander (Thorlabs Inc., Newton, MA, USA, GBE05-B) and attenuated by a variable iris diaphragm at approximately 0.5 cm in diameter. The intensity of the laser beam was controlled by rotating a half-wave plate (Thorlabs Inc., Newton, MA, USA; AHWP10M-980) mounted at the front of a laser-Glan polarizer (Thorlabs Inc., Newton, MA, USA, GL10-B) and attenuated to a 135 mW average power. The intensity of the beam was monitored by a digital power meter (Thorlabs Inc., Newton, MA, USA, PM100D) equipped with thermal power sensors (Thorlabs Inc., Newton, MA, USA, S470C).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!