Tracerlab fx c pro module

Manufactured by GE Healthcare

The TRACERlab FX-C Pro module is a lab equipment product designed for the synthesis of radiopharmaceuticals. It provides a platform for the automated and reliable production of radioactive tracer compounds used in medical imaging and diagnostic procedures.

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Lab products found in correlation

Gemini c18, by Phenomenex (1 mentions) Luna c18, by Phenomenex (1 mentions) Sorvall st 16r centrifuge, by Thermo Fisher Scientific (1 mentions) Pettrace 16.5 mev cyclotron, by GE Healthcare (1 mentions) Crc 25r, by Mirion Technologies (1 mentions) Gc 2010 plus, by Shimadzu (1 mentions) Fastlab synthesizer, by GE Healthcare (1 mentions)

6 protocols using tracerlab fx c pro module

Radiosynthesis of [11C]DASA-23 for PET Imaging

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Example 3

Radiosynthesis: Synthesis of [¹¹C]DASA-23 (FIGS. 2A and 9) was accomplished with a GE TRACERLab FX_{C Pro}module by reacting 4-((4-((2,6-difluorophenyl)sulfonyl)piperazin-1-yl)sulfonyl)phenol (2.4 μmol) with [¹¹C]methyl triflate in acetonitrile (300 μL) for 3 min at 80° C., using 5 N NaOH (24 μmol) as base. The reaction mixture was diluted with 1 mL water and loaded on a semi-prep HPLC for purification (Phenomenex Luna C18 5μ, 250×10 mm, 50% acetonitrile, 50% 0.1 M NH₄HCO₂with 0.5% AcOH; 7 mL/min). The fraction corresponding to [¹¹C]DASA-23 (retention time, t_R=9.5 min) was collected into a round flask preloaded with 20 mL water. The diluted aqueous fraction was passed through a pre-conditioned C-18 light Sep-Pak cartridge (Waters). The loaded cartridge was washed with sterile water (5 mL). [¹¹C]DASA-23 was eluted from the cartridge using ethanol (0.75 mL) and then saline (6.75 mL). The overall synthesis time was 52 min. Analytical HPLC (Phenomenex Gemini C18 5μ, 250×4.6 mm, 60% acetonitrile, 40% 0.1 M NH₄HCO₂with 0.5% AcOH, 1 mL/min) was used for assessment of radiochemical purity and specific activity.

US10039844B2. Imaging tumor glycolysis by non-invasive measurement of pyruvate kinase M2 (2018-08-07). The Board of Trustees of the Leland Stanford Junior University [US]. Inventors: Timothy Witney [GB], Michelle L. James [US], Sanjiv S. Gambhir [US].

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Radiolabeling of NOTA-PEG4-E[c(RGDfK)2] and [11C]Methionine

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The prodrug NOTA-PEG4-E [c (RGDfK)2] kit was purchased from Ruida Fuming Technology Co., Ltd. The ¹⁸F-fluoride in O-18 water was obtained from a cyclotron (HM-20 cyclotron, Sumitomo Heavy Machinery Co., Ltd., China People’s Liberation Army General Hospital). The radiolabeling of NOTA-PEG4-E [c (RGDfK)2] and [¹⁸F]AlF was performed following the previously published procedure with some modifications (Guo et al. 2014b (link)). The final product was designated [¹⁸F]Alfatide II ([¹⁸F]-ALF-NOTA-E [PEG 4-c (RGDFK)] 2). [¹¹C]methionine was synthesized by online [¹¹C]methylation of L-homocysteine on GE TRACERlab FX-C Pro module. The purity of [¹⁸F]Alfatide II and [¹¹C]methionine was determined by HPLC.

Pan Y., Dang H., Zhou H., Fu H., Wu S., Liu H., Zhang J., Wang R., Tian Y, & Xu B. (2024). A comparison study of dynamic [18F]Alfatide II imaging and [11C]MET in orthotopic rat models of glioblastoma. Journal of Cancer Research and Clinical Oncology, 150(4), 208.

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Radiosynthesis and Analysis of [11C]CO2

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[¹¹C]CO₂ was produced in a PET Trace^® 16.5 MeV cyclotron (GE Healthcare). A high-performance target was used for [¹¹C]CO₂ production. Target content was a mixture of N₂ and 1.0% O₂ (Praxair). Radiosynthesis was carried out using the TRACERlab^® FX C Pro module (GE Healthcare). The HPLC analyses were performed with a Shimadzu UFLC equipped with diode array and gamma detectors. The GC analyses of ethanol and residual solvents were done with a Shimadzu GC-2010 Plus equipped with a FID detector. Activity measurements were performed with a dose calibrator (Capintec CRC 25R, CRC 25 PET) or a 3” x 3” well type NaI(Tl) solid scintillation detector coupled to a multichannel analyser from ORTEC. Stability studies were conducted using a Thermo Scientific Sorvall ST 16R centrifuge.

Zoppolo F., Porcal W., Oliver P., Savio E, & Engler H. (2017). Automated One-pot Radiosynthesis of [11C]S-adenosyl Methionine. Current Radiopharmaceuticals, 10(3), 203-211.

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PET Imaging of AD Biomarkers

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PET with FDG and ¹¹C‐Pittsburgh compound‐B (PiB) was performed in every subject. The FDG PET acquisition protocol was used to evaluate parieto‐temporal AD hypometabolism and the Pittsburgh compound B PET acquisition protocol, to look for amyloid deposits in the brain. Synthesis of ¹¹C‐PiB was synthetized in a GE TRACER lab FXC PRO module, and lineal color scales used to detect metabolism as well as presence and spatial distribution of cortical amyloid.

Allegri R.F., Chrem Méndez P., Calandri I., Cohen G., Martín M.E., Russo M.J., Crivelli L., Pertierra L., Tapajóz F., Clarens M.F., Campos J., Nahas F.E., Vázquez S., Surace E, & Sevlever G. (2020). Prognostic value of ATN Alzheimer biomarkers: 60‐month follow‐up results from the Argentine Alzheimer's Disease Neuroimaging Initiative. Alzheimer's & Dementia : Diagnosis, Assessment & Disease Monitoring, 12(1), e12026.

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Radiochemical Production and Characterization

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All reagents were commercial products and used without further purification unless otherwise indicated. Animal studies were approved by the local institutional animal care and use committee and performed according to the Guide for the Care and Use of Laboratory Animals published by the U.S. National Institutes of Health (NIH publication 85-23, revised 1996 (20) ).
Radiochemistry 14 C-methionine was purchased from PerkinElmer. 11 C-methionine and 18 F-FDG were produced in-house with a 16-MeV Cyclotron (PETtrace 6; GE Healthcare), as described previously (21) . Briefly, 18 F-FDG was produced on a FASTlab synthesizer (GE Healthcare), and 11 C-methionine was synthesized on a TRACERlab FX-C Pro module (GE Healthcare) by an online 11 C-methylation of L-homocysteine. 18 F-fluorobenzyl triphenyl phosphonium, a myocardial perfusion PET tracer, was prepared as reported previously (22, 23) . All radiolabeled ligands were analyzed by high-performance liquid chromatography or thin-layer chromatography at the end of syntheses to confirm radiochemical identity and purity (.95%).

Maya Y., Werner R.A., Schütz C., Wakabayashi H., Samnick S., Lapa C., Zechmeister C., Jahns R., Jahns V, & Higuchi T. (2016). 11C-Methionine PET of Myocardial Inflammation in a Rat Model of Experimental Autoimmune Myocarditis. Journal of nuclear medicine : official publication, Society of Nuclear Medicine, 57(12).

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Automated Synthesis and Purification of 11C-PIB

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11C-PIB synthesis is carried out in a GE TRACER lab FXC PRO module which is a compact, automated radiochemistry system, which produces C-11 labeled radiochemicals from 11CeCO2. The module includes a HPLC (High Performance Liquid Chromatography) system for purification. The HPLC is a separation method that allows the isolation of the labeled product from radioactive by-products and organic impurities. In-house HPLC is performed with 0.009M sodium citrate ethanol/water (60/40) as a mobile phase with a flow rate of 3 mL/min and a reverse phase high performance liquid chromatographic (RP-HPLC) column. Chromatograms were registered using a UV-detector and a radioactivity detector in series. The product peak containing the [11C]-PIB is cut from the chromatographic system by valve switching from waste to product line. The [11C] PIB fraction (retention time 11-12 min) is transferred into flask and diluted with 30 mL of saline solution to reduce the ethanol percentage. The final total volume is 36 ml. The PIB solution is ready to be fractionated after sterile filtration. The10 mCi (adjusted by weight) was administered and image acquisition proceeded 50 min Post-11CPIB administration. Subsequently, dynamic tomographic images, 3D mode takes 20 min.

Duarte-Abritta B., Villarreal M.F., Abulafia C., Loewenstein D., Curiel Cid R.E., Castro M.N., Surace E., Sánchez S.M., Vigo D.E., Vázquez S., Nemeroff C.B., Sevlever G, & Guinjoan S.M. (2018). Cortical thickness, brain metabolic activity, and in vivo amyloid deposition in asymptomatic, middle-aged offspring of patients with late-onset Alzheimer's disease. Journal of psychiatric research, 107.

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