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Anti cd45.2 104

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The Anti-CD45.2 (104) is a laboratory instrument used for the detection and analysis of CD45.2 protein expression on cells. It is designed to provide researchers with a reliable tool for immunophenotyping and cell sorting applications.

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Lab products found in correlation

Anti cd45.1 a20, by BD (4 mentions) Anti b220 ra3 6b2, by BD (2 mentions) Anti p akt, by Cell Signaling Technology (1 mentions) Anti igd 11 26c 2a, by BD (1 mentions) Anti fas jo2, by BD (1 mentions) Rat igg2a isotype control 2a3, by BioXCell (1 mentions) Rat igg2b isotype control ltf 2, by BioXCell (1 mentions) Brdu flow kit, by BD (1 mentions) Anti ly6g 1a8, by BD (1 mentions) Anti siglecf e50 2440, by BD (1 mentions) Lsrfortessa flow cytometer, by BD (1 mentions) Facsdiva software, by BD (1 mentions) Anti cd11c n418, by Thermo Fisher Scientific (1 mentions) Anti mhcii m5 114, by Thermo Fisher Scientific (1 mentions) Anti cd45.1 a20, by BioLegend (1 mentions) Anti cd34 ram34, by Thermo Fisher Scientific (1 mentions) Anti cd16 32 2.4g2, by BD (1 mentions) Anti cd4 rm4 5, by BD (1 mentions) Anti cd43 s7, by BD (1 mentions) Anti ter119 ter 119, by BioLegend (1 mentions)

Spelling variants of this product

Anti-CD45 (213 citations) CD45.2 (104, (10 citations) Anti-CD45.2 (clone 104, (4 citations) Biotin–anti-CD45.2 (104; (2 citations) Anti-CD45.2-Alexa Fluor 700 mAb (clone 104) (2 citations) Anti-CD45.2-FITC (clone 104; (2 citations)

5 protocols using anti cd45.2 104

1

Multiparameter Flow Cytometry Analysis of Germinal Center B Cells

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Spleen and lymph nodes were isolated and mashed into media containing
2% FCS. For analysis of GC B cells, cells were stained with anti-B220
(RA3-6B2; BD Biosciences or Biolegend); anti-CD19 (6D5; BD Biosciences);
anti-Fas (Jo2; BD Biosciences); anti-IgD (11-26c.2a; BD Biosciences or
Biolegend); anti-CD45.1 (A20; BD Biosciences or Biolegend); anti-CD45.2 (104; BD
Biosciences or Biolegend); anti-IgG2b (RMG2b-1; BD Biosciences); homemade
Alexa647 conjugated DEL; antibody to T cell and B cell activation antigen (GL7;
BD Biosciences); anti-Mouse Eα52-68 peptide bound to I-Ab(Y-Ae, eBioscience); anti-integrin β1 (MB1.2; Chemicon); anti-integrin
β2 (C71/16; BD Biosciences); anti-integrin β3 (2C9.G2;
Biolegend); anti-integrin β7 (M293; BD Biosciences); anti-integrin
α4 (PS/2; Bio X Cell); anti-integrin αL (M17/4; Bio X
Cell); anti-integrin αV (RMV-7; BD Biosciences); rat IgG2a
isotype control (2A3; Bio X Cell); rat IgG2b isotype control (LTF-2; Bio X Cell)
or rat IgG1 isotype control (R3-34; BD Biosciences). BrdU staining was done
using BrdU flow kit (BD Biosciences) following manufacturer's
instructions. For intracellular staining of phosphorylated AKT at Ser473 (pAKT),
cells were instantly fixed and stained as described (35 (link)). Anti-pAKT (9271; Cell Signaling Technology) was
used.
Wang X., Rodda L., Bannard O, & Cyster J.G. (2014). Integrin-mediated interactions between B cells and follicular dendritic cells influence germinal center B cell fitness. Journal of immunology (Baltimore, Md. : 1950), 192(10), 4601-4609.
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2

Lung Cell Phenotyping in Chimeric Mice

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Preparation of lung cells for the determination of cell exchange in chimeric mice has been described [62 (link)]. The lung cell suspension was labelled with anti-panCD45 (30-F11, eBioscience), anti-CD45.1 (A20, BD Pharmingen), anti-CD45.2 (104, BD Pharmingen), anti-Ly6G (1A8, BD Pharmingen), anti-CD11c (N418, eBioscience) anti-MHC-II (M5/114.15.2, eBioscience), anti-Siglec-F, (E50-2440, BD Pharmingen) and anti-CD64 (X54-5/7.1, BD Pharmingen). Cells were analyzed on a Becton Dickinson LSRFortessa flow cytometer using FACSDIVA software (BD Biosciences).
Naujoks J., Tabeling C., Dill B.D., Hoffmann C., Brown A.S., Kunze M., Kempa S., Peter A., Mollenkopf H.J., Dorhoi A., Kershaw O., Gruber A.D., Sander L.E., Witzenrath M., Herold S., Nerlich A., Hocke A.C., van Driel I., Suttorp N., Bedoui S., Hilbi H., Trost M, & Opitz B. (2016). IFNs Modify the Proteome of Legionella-Containing Vacuoles and Restrict Infection Via IRG1-Derived Itaconic Acid. PLoS Pathogens, 12(2), e1005408.
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3

Hematopoietic Stem Cell Chimerism Analysis

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Bone marrow was isolated from and Hsf1f/fMx1-Cre+ (CD45.2+) donor mice and support Hsf1+/+Mx1-Cre+ (CD45.1+) donor mice. Donor cells (1 × 106 per mouse) were mixed at a ratio 1:1 with support bone marrow cells and transplanted via retro-orbital injection into lethally irradiated (550 rads, twice) CD45.1+ recipient mice. Two weeks after transplantation, mice were injected with poly(I:C) to induce deletion by Mx1-Cre (see above). Chimerism was monitored by flow cytometry of peripheral blood (with anti-CD45.1 (A20; BD Bioscience) and anti-CD45.2 (104; BD Bioscience)) at 4-week intervals after transplantation for 20 weeks, at which time mice were sacrificed for assessment of chimerism in bone marrow and spleen.
Kourtis N., Lazaris C., Hockemeyer K., Balandrán J.C., Jimenez A.R., Mullenders J., Gong Y., Trimarchi T., Bhatt K., Hu H., Shrestha L., Ambesi-Impiombato A., Kelliher M., Paietta E., Chiosis G., Guzman M.L., Ferrando A.A., Tsirigos A, & Aifantis I. (2018). Oncogenic hijacking of the stress response machinery in T cell acute lymphoblastic leukemia. Nature medicine, 24(8), 1157-1166.
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4

Hematopoietic Stem Cell Chimerism Analysis

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Bone marrow was isolated from and Hsf1f/fMx1-Cre+ (CD45.2+) donor mice and support Hsf1+/+Mx1-Cre+ (CD45.1+) donor mice. Donor cells (1 × 106 per mouse) were mixed at a ratio 1:1 with support bone marrow cells and transplanted via retro-orbital injection into lethally irradiated (550 rads, twice) CD45.1+ recipient mice. Two weeks after transplantation, mice were injected with poly(I:C) to induce deletion by Mx1-Cre (see above). Chimerism was monitored by flow cytometry of peripheral blood (with anti-CD45.1 (A20; BD Bioscience) and anti-CD45.2 (104; BD Bioscience)) at 4-week intervals after transplantation for 20 weeks, at which time mice were sacrificed for assessment of chimerism in bone marrow and spleen.
Kourtis N., Lazaris C., Hockemeyer K., Balandrán J.C., Jimenez A.R., Mullenders J., Gong Y., Trimarchi T., Bhatt K., Hu H., Shrestha L., Ambesi-Impiombato A., Kelliher M., Paietta E., Chiosis G., Guzman M.L., Ferrando A.A., Tsirigos A, & Aifantis I. (2018). Oncogenic hijacking of the stress response machinery in T cell acute lymphoblastic leukemia. Nature medicine, 24(8), 1157-1166.
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5

Monoclonal Antibodies for Hematopoietic Cell Analysis

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The following monoclonal antibodies (Abs) were used for flow cytometry and cell sorting: anti-c-Kit Microbeads (Miltenyi Biotec), anti-c-Kit (2B8, BD Biosciences / Biolegend), anti-Sca-1 (E13–161.7, BD Biosciences / Biolegend), anti-CD45.1 (A20, Biolegend), anti-CD45.2 (104, BD Biosciences), anti-CD4 (RM4–5, BD Biosciences), anti-B220 (RA3–6B2, BD Biosciences), anti-TER-119 (TER-119, Biolegend), anti-Gr-1 (RB6–8C5, BD Biosciences/Biolegend), anti-Mac-1 (M1/70, BD Biosciences), anti-CD71 (C2, BD Biosciences), anti-CD43 (S7, BD Biosciences), anti-CD16/32 (2.4G2, BD Biosciences), anti-Ki67 (B56, BD Biosciences), anti-CD34 (RAM34, eBioscience), anti-CD135 (A2F10, Biolegend), The primary Ab used for immunocytochemistry (ICC) was goat anti-Tfe3 (p-16; Santa Cruz Biotechnology). Anti-phospho mTor (Ser2448), anti-phospho Akt (Thr308), and anti-beta actin from Cell Signaling were used for western blotting.
Baba M., Toyama H., Sun L., Takubo K., Suh H.C., Hasumi H., Nakamura-Ishizu A., Hasumi Y., Klarmann K.D., Nakagata N., Schmidt L.S., Linehan W.M., Suda T, & Keller J.R. (2016). Loss of Folliculin disrupts hematopoietic stem cell quiescence and homeostasis resulting in bone marrow failure. Stem cells (Dayton, Ohio), 34(4), 1068-1082.
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