Resomer rg502h

Manufactured by Boehringer Ingelheim

Sourced in Germany

Resomer RG502H is a biodegradable polymer material. It is composed of a 50:50 copolymer of lactic acid and glycolic acid. Resomer RG502H is designed for use in the manufacture of medical devices and pharmaceutical formulations.

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Lab products found in correlation

14 protocols using resomer rg502h

Polymeric Nanoparticle Formulation

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QU, PVA (average MW 30000–70000) were purchased from Sigma-Aldrich (USA). PLGA (50:50, Resomer RG 502 H, MW 13100) was purchased from Boehringer Ingelheim (Germany). All other chemicals used were of analytical grade and the solvents were of HPLC grade.

TEFAS L.R., TOMUŢĂ I., ACHIM M, & VLASE L. (2015). Development and optimization of quercetin-loaded PLGA nanoparticles by experimental design. Clujul Medical, 88(2), 214-223.

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Polymeric Nanoparticles for Drug Delivery

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The two PLGA polymers, Resomer RG502 (ester terminated) and Resomer RG502H (acid terminated), and the PLGA/PEG copolymer (RGP d50105) were obtained from Boehringer Ingelheim (Ingelheim am Rhein, Germany). Dichloromethane, ethanol, methanol, 30,000–70,000 MW polyvinyl alcohol (PVA), bovine serum albumin (BSA), HEPES, glucose, sodium chloride, potassium chloride, calcium chloride, magnesium chloride, sodium phosphate monobasic, potassium phosphate dibasic, sodium hydroxide, hydrochloric acid, formic acid, Hanks’ Balanced Salt Solution (HBSS), and Lucifer yellow were obtained from Sigma Aldrich (St. Louis, MO). Fenretinide was purchased from R&D Systems, Inc. (Minneapolis, MN). All cell culture media were purchased from Thermo Fisher Scientific (Waltham, MA).

Graves R.A., Ledet G.A., Glotser E.Y., Mitchner D.M., Bostanian L.A, & Mandal T.K. (2015). Formulation and Evaluation of Biodegradable Nanoparticles for the Oral Delivery of Fenretinide. European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 76, 1-9.

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Curcumin-loaded PLGA and PCL Nanoparticles

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Curcumin (78% purity by HPLC) was provided by Sigma-Aldrich (Saint Quentin-Fallavier, France). Poly(d,l-lactide-co-glycolide) (PLGA; Resomer^® RG 502 H) was obtained from Boehringer-Ingelheim (Ingelheim am Rhein, Germany). Polycaprolactone (PCL was purchased from Sigma-Aldrich (Saint Quentin-Fallavier, France). Eudragit^® RLPO was a gift from Evonik-IMCD (Darmstadt, Germany). Polymer specifications according to manufacturers’ data are presented in Table 1 and FTIR spectra of PCL, PLGA and ERL are shown in the Supplementary Materials (Figure S1). Poly(vinyl alcohol) (PVA, average molecular weight 30,000–70,000; 87–90% hydrolysed), MTT and cell culture reagents were provided by Sigma-Aldrich (Saint Quentin-Fallavier, France). All other chemicals and solvents were of analytical grade.

Umerska A., Gaucher C., Oyarzun-Ampuero F., Fries-Raeth I., Colin F., Villamizar-Sarmiento M.G., Maincent P, & Sapin-Minet A. (2018). Polymeric Nanoparticles for Increasing Oral Bioavailability of Curcumin. Antioxidants, 7(4), 46.

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Formulation and Characterization of PLGA Nanoparticles

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PLGA (Resomer RG 502 H, lactide:glycolide molar ratio 48:52 to 52:48) was purchased from Boehringer Ingelheim, Germany. Solvents used for PLGA preparation were obtained from Sigma-Aldrich (Zwijndrecht, The Netherlands). The lipids were purchased from Avanti Polar Lipids (Alabaster, AL, USA) and included 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amine (polyethylene glycol)-2000] (ammonium salt) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt) (mPEG 2000 PE). Poly (inosinic:cytidylic acid (poly(I:C)) and the near-infrared (NIR) dye IR-780 were purchased from Sigma-Aldrich (Zwijndrecht, The Netherlands), R848 was obtained from Alexis Biochemicals (Paris, France), and MIP3α (CCL20) was purchased from R&D Systems (Minneapolis, MN, USA).

Huis in ‘t Veld R.V., Da Silva C.G., Jager M.J., Cruz L.J, & Ossendorp F. (2021). Combining Photodynamic Therapy with Immunostimulatory Nanoparticles Elicits Effective Anti-Tumor Immune Responses in Preclinical Murine Models. Pharmaceutics, 13(9), 1470.

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PLGA Nanoparticle Formulation and Characterization

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PLGA (Resomer RG 502 H, lactide/glycolide molar ratio 48:52 to 52:48) was purchased from Boehringer Ingelheim (Germany). Solvents for PLGA preparation (dichloromethane) were obtained from Merck (Germany). Polyvinyl alcohol (PVA) was obtained from Sigma (The Netherlands). R848 was from Enzo Life Sciences poly I:C from Sigma-Aldrich, and endotoxin-free OVA from Hyglos. OVA (257 – 264) SIINFEKL and HPV16 E7(49–57) obtained from Anaspec. α-GalCer was purchased from Funakoshi Co. Ltd. Dimethyl sulfoxide (DMSO) ≥99.9% and Tween 20 was obtained from Sigma (The Netherlands). Atto647N was obtained from Atto-tec GmbH. RPMI 1640 medium (Life Technologies Inc..). PE-conjugated CD3, APC-Cy7 conjugated CD45.2 (BD Biosciences), PerCP conjugated CD8⁺α (Biolegend), and APC-conjugated H2-Kb/SIINFEKL-Tetramer (Sanquin), PE conjugated CD1d- α-GalCer dextramer (Immudex),FITC conjugated CD11b, PE/Cy7 conjugated CD11c, Alexa488 conjugated F4/80 and NK1.1 (Biolegend), PE/Cy7 conjugated CD154 (Biolegend), Celltrace CFSE, Celltrace- violet and Celltrace red (Life technologies Inc.), were used in flow cytometric cell staining. For the depletion studies, anti-mouse CD4⁺ antibody clone GK1.5 was obtained from BioXcell. For the detection of CD4⁺ populations PerCP/Cy5.5 conjugated anti-mouse CD4⁺ antibody clone RM4-4 was obtained from Biolegend.

Dölen Y., Kreutz M., Gileadi U., Tel J., Vasaturo A., van Dinther E.A., van Hout-Kuijer M.A., Cerundolo V, & Figdor C.G. (2015). Co-delivery of PLGA encapsulated invariant NKT cell agonist with antigenic protein induce strong T cell-mediated antitumor immune responses. Oncoimmunology, 5(1), e1068493.

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Polymeric Drug Delivery Systems

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Poly(d,l-lactide-co-glycolide) (PLGA) polymer, Resomer RG 752H (lactide:glycolide: 75:25, inherent viscosity 0.14–0.22 dL/g, M_w = 4000–15,000), and Resomer RG 502H (lactide:glycolide: 50:50, inherent viscosity 0.16–0.24 dL/g, M_w = 7000–17,000 g/mol) were produced by Boehringer Ingelheim (Ingelheim am Rhein, Germany). PEGylated-PLGA (PEG-PLGA) polymer, Resomer, RGP d 5055 (PEG-PLGA) (PEG content: 3–7% (m/m), inherent viscosity: 0.93 dL/g, M_w = 33,500 g/mol) was obtained from Evonik (Essen, Germany). Polyvinyl alcohol (PVA, M_w = 30,000–70,000 g/mol, 87–90% hydrolysed), dichloromethane (DCM), acetone, glacial acetic acid, dimethyl sulfoxide (DMSO), sodium azide, 1-ethyl-3(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS), sodium dodecyl sulphate (SDS), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), piroxicam, and RPMI-1640 medium were obtained from Sigma Aldrich (St. Louis, MO, USA). Sorafenib (free base) and doxorubicin HCl were purchased from Active Biochem (Hong Kong, China). Cyanine 5 amine was produced by Lumiprobe GmBH (Hannover, Germany).

Babos G., Biró E., Meiczinger M, & Feczkó T. (2018). Dual Drug Delivery of Sorafenib and Doxorubicin from PLGA and PEG-PLGA Polymeric Nanoparticles. Polymers, 10(8), 895.

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PLGA-based Nanoparticle Synthesis Protocol

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Poly (D,L-lactide-co-glycolide) (PLGA) (50:50 Resomer RG 502H inherent viscosity 0.16–0.24 dL/g) was purchased from Boehringer Ingelheim (Ingelheim, Germany). Polyethyleneimine (PEI, MW = 10–25 kDa branched), poloxamer 188 (Pluronic® F68), Bradford reagent, potassium phosphate dibasic and potassium phosphate monobasic, sodium azide, sodium chloride, copper (II) sulphate and albumin from chicken egg white (OVA), were purchased from Sigma-Aldrich. Sodium hydroxide was provided from Delchimica Scientific Glassware. Ethanol (96 %), phosphoric acid (85 %), acetic acid, acetonitrile and tetrahydrofuran were purchased from Carlo Erba Reagenti (Milan, Italy).

Petrizzo A., Conte C., Tagliamonte M., Napolitano M., Bifulco K., Carriero V., De Stradis A., Tornesello M.L., Buonaguro F.M., Quaglia F, & Buonaguro L. (2015). Functional characterization of biodegradable nanoparticles as antigen delivery system. Journal of Experimental & Clinical Cancer Research : CR, 34, 114.

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Formulation of 5-Fu-loaded PLGA Nanoparticles

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5-Fu was purchased from Sigma Chemical Company (St Louis, MO, USA). PLGA polymer (Resomer RG502H) was purchased from Boehringer-Ingelheim (Ingelheim, Germany). The mole ratio of glycolic acid to lactic acid in PLGA was 50:50 and its molecular weight was 12000 g/mol. Ferrous chloride (FeCl2.4H2O), ferric chloride (FeCl3.6H2O), oleic acid, ammonia solution (25 vol%), dichloromethane (DCM), glycerin, span60 and tween60 were purchased from Merck Chemical Company (Darmstadt, Germany). All chemicals were used without further purification.

Abed Z., Beik J., Khoee S., Khoei S., Shakeri-Zadeh A, & Shiran M.B. (2016). Effects of Ultrasound Irradiation on the Release Profile of 5-fluorouracil from Magnetic Polylactic co-glycolic Acid Nanocapsules. Journal of Biomedical Physics & Engineering, 6(3), 183-194.

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PLGA-PEI Nanoparticle Formulation

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PLGA (Resomer^® RG502H, lactic:glycolic molar ratio 50:50) was purchased from Boehringer Ingelheim (Ingelheim, Germany). PEI (branched form, molecular weight 25 kDa), chicken-egg OVA, and polyvinyl alcohol (80% hydrolyzed, molecular weight 9,000–10,000 Da) were purchased from Sigma-Aldrich (St Louis, MO, USA). Fluorescein isothiocyanate (FITC)-conjugated OVA (FITC-OVA) was acquired from Thermo Fisher Scientific (Waltham, MA, USA).

Song C., Noh Y.W, & Lim Y.T. (2016). Polymer nanoparticles for cross-presentation of exogenous antigens and enhanced cytotoxic T-lymphocyte immune response. International Journal of Nanomedicine, 11, 3753-3764.

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PLGA-PEG Nanomaterial Synthesis

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Equimolar uncapped poly(d,l-lactide-co-glycolide) (PLGA) (Resomer RG502H, average Mw 12 kDa) was purchased from Boehringer Ingelheim (Ingelheim, Germany). Polyethylene glycol (PEG, Mw 1500 Da), Ethylenediamine (en), N,N-diisopropylethylamine (DIPEA), O-benzotriazole-N,N,N′,N′-tetramethyluronium-hexafluoro-phosphate (HBTU), Ethyl 2-cyano-2-(hydroxyimino) acetate (Oxyma Pure Novabiochem®), anhydrous N,N-dimethyl-formamide (DMF), Acetonitrile (ACN), N,N′-Diisopropylcarbodiimide (DIC), N,N′-Dicycloexylcarbodiimide (DCC), Rhodamine B, 4-(dimethylamino) pyridine (DMAP), Dichloromethane (DCM), Dimethylsulfoxide (DMSO), Sodium carbonate (Na₂CO₃), Piperidine, Trifluoroacetic acid (TFA), Formic acid, HPLC-grade water, and all buffer solutions were purchased from Sigma-Aldrich S.r.l.(Milan, Italy). All Fmoc-aminoacids were purchased from IRIS Biotech GmbH (Marktredwitz, Germany). Dialysis bag MWCO 6000–8000 Da were purchased from Spectrum Europe B.V. (Breda, Netherlands). Distilled and deionized water used Millipore Milli-RO 10 Plus from Merck S.p.a. (Milan, Italy) at 18 MΩ resistance. Amicon^® Ultra-4 centrifugal filters were purchased from Merck S.p.a. (Milan, Italy).

Falanga A.P., Melone P., Cagliani R., Borbone N., D’Errico S., Piccialli G., Netti P.A, & Guarnieri D. (2018). Design, Synthesis and Characterization of Novel Co-Polymers Decorated with Peptides for the Selective Nanoparticle Transport across the Cerebral Endothelium. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(7), 1655.

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