Sod assay kit

Manufactured by Nanjing Jiancheng

Sourced in China

The SOD assay kit is a laboratory tool used to measure the activity of the enzyme superoxide dismutase (SOD) in biological samples. SOD is an important antioxidant enzyme that plays a crucial role in protecting cells from oxidative stress. The kit provides a reliable and efficient method to quantify SOD levels, which can be useful for various research and diagnostic applications.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

SOD kit (68 citations) SOD and MDA assay kits (24 citations) Malondialdehyde (MDA) and superoxide dismutase (SOD) assay kits (7 citations) Assay kits for SOD (2 citations) SOD and GSH-Px assay kits (2 citations)

198 protocols using sod assay kit

Yeast Antioxidant Enzyme Activity Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

BY4741 yeast cells were cultured in liquid glucose medium to reach the logarithmic growth phase. The cells were divided into five groups with OD600 value of 0.1 and treated with GPS at 0, 1, 3, and 10 μM or Res at 10 μM and cultured in a glucose medium for 24 h. Subsequently, the yeast cells from different groups were collected and sonicated for 5 min. The cell lysates were centrifuged at 12,000 g for 15 min, and the supernatant was obtained to test the activity of enzymes. The activities of CAT, GPx, and SOD were determined with CAT and GPx assay kits (Beyotime Biotechnology Limited Company, Shanghai, China) and SOD assay kits (Nanjing Jiancheng Bioengineering Institute (Nanjing, China) in accordance with the manufacturer's instructions. All experimental procedures were conducted in strict accordance with the protocol instructions provided by the manufacturers.

Liu Q., Cheng L., Matsuura A., Xiang L, & Qi J. (2020). Gentiopicroside, a Secoiridoid Glycoside from Gentiana rigescens Franch, Extends the Lifespan of Yeast via Inducing Mitophagy and Antioxidative Stress. Oxidative Medicine and Cellular Longevity, 2020, 9125752.

+ Open protocol

+ Expand

Cardioprotective Effects of DOX Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

DOX was provided by Lingnan Pharmaceutical, Ltd. (Guangzhou, China). Ber was provided by Acros Organics (Geel, Belgium). Fluo3-AM was purchased from Sigma-Aldrich; Merck KGaA (Darmstadt, Germany). MDA, CAT and SOD assay kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). CK and CK-MB assay kits were purchased from Sysmex Corporation (Kobe, Japan). Rhodamine (Rh-123) and Rhod-2-acetoxymethyl (rhod-2-AM) were purchased from Molecular Probes; Thermo Fisher Scientific, Inc., (Waltham, MA, USA). The working solutions of Harris Haematoxylin and eosin were purchased from Baso Diagnostics Inc. Zhuhai (New Taipei City, Taiwan).

Xiong C., Wu Y.Z., Zhang Y., Wu Z.X., Chen X.Y., Jiang P., Guo H.C., Xie K.R., Wang K.X, & Su S.W. (2018). Protective effect of berberine on acute cardiomyopathy associated with doxorubicin treatment. Oncology Letters, 15(4), 5721-5729.

+ Open protocol

+ Expand

Oxidative Stress Biomarkers in Hippocampus

Check if the same lab product or an alternative is used in the 5 most similar protocols

Malondialdehyde (MDA) assay was performed to measure lipid peroxidation. The level of superoxide dismutase (SOD) activity indirectly reflects the ability of cells to remove ROS. The hippocampal tissues were isolated from the brain, homogenized with lysis buffer, and centrifuged to get the supernatant. The MDA and SOD activity levels were detected using commercially available MDA assay kits (Jiancheng Biotech, Nanjing, China) and SOD assay kits (Jiancheng Biotech, Nanjing, China) according to the manufacturer’s protocols.

Yuan Z., Lu L., Lian Y., Zhao Y., Tang T., Xu S., Yao Z, & Yu Z. (2022). AA147 ameliorates post-cardiac arrest cerebral ischemia/reperfusion injury through the co-regulation of the ATF6 and Nrf2 signaling pathways. Frontiers in Pharmacology, 13, 1028002.

+ Open protocol

+ Expand

Lipid Peroxidation and Antioxidant Activity Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

The left lower lobe lung samples were homogenized in chilled phosphate-buffered saline (PBS); the homogenate was then centrifuged at 5000 rpm for 10 min at 4°C. The supernatant was used to determine MDA and SOD. MDA assay reagents and SOD assay kits were purchased from the Nanjing Jiancheng Bioengineering Institute (Nanjing, China). MDA concentrations were determined by the thiobarbituric acid method and SOD activities were evaluated by the xanthine oxidase method. The absorbance was measured at 532 and 550 nm for MDA and SOD, respectively, with a spectrometer.

Chen X., Liu Q., Wang D., Feng S., Zhao Y., Shi Y, & Liu Q. (2015). Protective Effects of Hydrogen-Rich Saline on Rats with Smoke Inhalation Injury. Oxidative Medicine and Cellular Longevity, 2015, 106836.

+ Open protocol

+ Expand

Measuring Antioxidant Enzymes and MDA

Check if the same lab product or an alternative is used in the 5 most similar protocols

According to the manufacturer’s instructions, the levels of Superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and malondialdehyde (MDA) were measured with commercially available SOD assay kits, POD assay kit, CAT assay kit, and MDA assay kit (Jiancheng Bioengineering Institute, Nanjing, China), respectively.

Peng T., Wang Y., Yang T., Wang F., Luo J, & Zhang Y. (2021). Physiological and Biochemical Responses, and Comparative Transcriptome Profiling of Two Angelica sinensis Cultivars Under Enhanced Ultraviolet-B Radiation. Frontiers in Plant Science, 12, 805407.

+ Open protocol

+ Expand

Evaluation of Antioxidant Enzyme Activities

Check if the same lab product or an alternative is used in the 5 most similar protocols

Trypsin was from Promega, Gaungzhou, China. PMSF (Phenylmethanesulfonyl fluoride) was from Solarbio, Guangzhou, China. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), thiocarbamide, DTT (dithiothreitol) and iodoacetamide were obtained from Sigma-Aldrich Co. LLC., Shanghai, China. MDA, GSH-Px, CAT and SOD assay kits were purchased from Nanjing Jiancheng Bioengineering Institute, Nanjing, China. Dulbecco’s modified Eagle’s medium (DMEM), Fetal bovine serum (FBS), and the antibiotic mixture (penicillin-streptomycin) were purchased from Jianyang Biotechnology Co., Ltd., Guangzhou, China. Rabbit anti-Haptoglobin, anti-Cytochrome c, anti-Nucleophosmin, anti-HSP60, anti-CA3, anti-PDHA1, anti-GAPDH were purchased from Bioworlde, Guangzhou, China. Goat anti-rabbit IgG-HRP was from Santa Cruz, CA, USA. Other reagents were of analytical grade.

Zeng Q., Jiang J., Wang J., Zhou Q, & Zhang X. (2019). N-Terminal Acetylation and C-Terminal Amidation of Spirulina platensis-Derived Hexapeptide: Anti-Photoaging Activity and Proteomic Analysis. Marine Drugs, 17(9), 520.

+ Open protocol

+ Expand

Oxidative Stress Assessment Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lactate dehydrogenase (LDH) release, superoxide dismutase (SOD) activity, and malondialdehyde content were assessed via colorimetric assays, as measures of oxidative stress. Three days after irradiation the supernatant of the culture medium was collected, and LDH release was measured using a commercially available LDH assay kit (Nanjing Jiancheng, Nanjing, China) in accordance with the manufacturer’s instructions. SOD activity and malondialdehyde content in cells were assessed via SOD assay kits and malondialdehyde assay kits (Nanjing Jiancheng).

Huang K., Zhao W., Wang X., Qiu Y., Liu Z., Chen R., Liu W, & Liu B. (2021). Akt Inhibition Enhanced the Growth Inhibition Effects of Low-Dose Heavy-Ion Radiation via the PI3K/Akt/p53 Signaling Pathway in C6 Glioblastoma Cells. Frontiers in Oncology, 11, 649176.

+ Open protocol

+ Expand

Mitochondrial Bioenergetics and Oxidative Stress Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mitochondrial ATP activity was assayed using an ATP bioluminescence assay kit (Beyotime Institute of Biotechnology, China), according to the manufacturer's instructions. Mitochondrial transmembrane potential (△Ψm) in HPMECs was measured as the manufacturer's direction. Briefly, 25 nmol/L TMRM dye (Molecular Probes, Invitrogen, USA) was added to ECM medium for 30 min and assessed via spectrophotometer (SpectraMax 5; Molecular Devices, Sunnyvale, CA, USA). HPMECs were treated with salmon sperm DNA (10 μg/mL) for 2 h and then mitochondrial ROS was detected using MitoSOX (Invitrogen, USA) according to the manufacturer's instructions and measured via spectrophotometer. MPO, MDA, SOD and total antioxidant capacity were measured by MPO assay kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China), MDA assay kits (Beyotime Institute of Biotechnology, China), SOD assay kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) and a rapid ABTS method (Beyotime Institute of Biotechnology, China), respectively.

Cen M., Ouyang W., Zhang W., Yang L., Lin X., Dai M., Hu H., Tang H., Liu H., Xia J, & Xu F. (2021). MitoQ protects against hyperpermeability of endothelium barrier in acute lung injury via a Nrf2-dependent mechanism. Redox Biology, 41, 101936.

+ Open protocol

+ Expand

Oxidative Stress Quantification in Lung and Spleen

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tissues of the lung and spleen were cut into cubes, and the dispersed cells filtered with a 300-mesh nylon net. After washing with cold PBS, the fluorescence intensity of ROS was measured with excitation wavelength at 500 nm and emission wavelength at 525 nm using reactive oxygen species assay kits (Nanjing Jiancheng Bioengineering Institute, China) following the manufacturer's protocols.
Tissues of the lung and spleen were homogenized using a tissue grinder and determined using BCA Protein Assay Kits. The activity of SOD was assessed using SOD assay kits (Nanjing Jiancheng Bioengineering Institute) according to the manufacturer's instructions.

Liu Y., Wang K., Feng Q., Zhang Y., Wang C., Liu Q., Liu X., Wang X., Gao W., Bai X., Li Z, & Wang Y. (2021). Alpinetin Attenuates Persistent Inflammation, Immune Suppression, and Catabolism Syndrome in a Septic Mouse Model. Journal of Immunology Research, 2021, 9998517.

+ Open protocol

+ Expand

Oxidative Stress Analysis in Frozen Liver

Check if the same lab product or an alternative is used in the 5 most similar protocols

Frozen liver samples were prepared and centrifuged as described for determination of ATP levels, subsequently, the supernatants were collected and used immediately for MDA content and SOD activity assays, according to the standard protocol of the MDA assay (cat. no. A003-1; Nanjing Jiancheng Bioengineering Institute) and SOD assay kits (cat. no. A001-1-1; Nanjing Jiancheng Bioengineering Institute).

Zeng C., Hu X., He W., Wang Y., Li L., Xiong Y, & Ye Q. (2017). Hypothermic machine perfusion ameliorates inflammation during ischemia-reperfusion injury via sirtuin-1-mediated deacetylation of nuclear factor-κB p65 in rat livers donated after circulatory death. Molecular Medicine Reports, 16(6), 8649-8656.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!