Mvx10 fluorescence microscope

Manufactured by Olympus

Sourced in Japan, China

The MVX10 is a fluorescence microscope designed for high-resolution imaging. It features an advanced optical system that provides clear, detailed images of fluorescently labeled samples. The microscope is equipped with various illumination options, allowing users to choose the appropriate light source for their specific application.

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Lab products found in correlation

Di 4 anepps, by Thermo Fisher Scientific (2 mentions) Anti myc, by Proteintech (1 mentions) Alexa fluor 555, by Thermo Fisher Scientific (1 mentions) 5 ethynyl 2 deoxyuridine (edu), by Thermo Fisher Scientific (1 mentions) Axio imager a2 light microscope, by Zeiss (1 mentions) Sp8 confocal microscope, by Leica (1 mentions) Mvx10, by Olympus (1 mentions)

Spelling variants of this product

Fluorescence microscope (5377 citations) MVX10 (328 citations) MVX10 microscope (103 citations) MVX10 fluorescence stereo microscope (10 citations) MVX10 MacroView fluorescence microscope (6 citations) MVX10 Fluorescence MacroZoom dissecting microscope (3 citations) MVX10 MacroZoom fluorescence microscope (2 citations)

9 protocols using mvx10 fluorescence microscope

Voltage-Sensitive Dye Imaging of Tissue Contraction

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Tissues were treated with 2 μM
voltage sensitive dye, Di-4-ANEPPS (Invitrogen) in DMEM for 30 min
at room temperature. The tissues were paced at 1.5–2 Hz with
a Pulsar 6i Stimulator (FHC, Inc.) at 2× their excitation threshold.
Tissue contraction was stopped by 20 min treatment with 10 μM
blebbistatin. Dye fluorescence was recorded on an MVX-10 Olympus fluorescence
microscope (Olympus Corporation) equipped with a charged coupled device
(CCD) (Cascade 128, Photometrics). The 1 cm sensor had 128 ×
128 pixel resolution. Recordings were performed at 500 frames/s with
0 exposure time. Maps were generated by scroll software.

Wang E.Y., Rafatian N., Zhao Y., Lee A., Lai B.F., Lu R.X., Jekic D., Davenport Huyer L., Knee-Walden E.J., Bhattacharya S., Backx P.H, & Radisic M. (2019). Biowire Model of Interstitial and Focal Cardiac Fibrosis. ACS Central Science, 5(7), 1146-1158.

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Voltage Sensitive Dye Imaging of Tissue

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Tissues were perfused with 5μM voltage sensitive dye, Di-4-ANEPPS (Invitrogen), in Kreb’s Solution at 35–37 °C for 20min. Dye fluorescence was recorded on an MVX-10 Olympus fluorescence microscope (Olympus Corporation) equipped with a charged coupled device (CCD) (Cascade 128, Photometrics). The 1cm sensor had 128×128 pixel resolution. Recordings were performed at 500 frames/s with 0 exposure time. Biowires were paced at 1.5–2Hz with a Pulsar 6i Stimulator (FHC, Inc.) at twice the ET.

Zhao Y., Rafatian N., Feric N.T., Cox B., Aschar-Sobbi R., Wang E.Y., Aggarwal P., Zhang B., Conant G., Pahnke K.A., Protze S., Lee J.H., Davenport Huyer L., Jekic D., Wickeler A., Naguib H., Keller G.M., Vunjak-Novakovic G., Broeckel U., Backx P.H, & Radisic M. (2019). A platform for generation of chamber specific cardiac tissues and disease modelling. Cell, 176(4), 913-927.e18.

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Visualizing Hox Gene Expression in Transgenic Embryos

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The Tg(drl:hoxa9) embryos at 28-somite stage were fixed in 4% PFA plus 1% DMSO in 0.1 M phosphate buffer (PB). Embryos were then washed three times using PB and dehydrated in MeOH at −20°C, followed by pre-chilled acetone treatment for 7–20 min at −20°C. Embryos were then washed with incubation buffer (IB, 0.1 M PB buffer containing 0.2% BSA and 0.5% Triton-X) three times. After that, embryos were incubated with anti-myc (Proteintech, 60003-2-1g, China) overnight at 4°C, washed with IB and then incubated with 594-conjugated goat anti-mouse IgG (H + L) secondary antibody (Proteintech, SA00013-3, China). The stained embryos were imaged under a MVX10 fluorescence microscope (Olympus, Japan).

Wang W., Li H., Huang M., Wang X., Li W., Qian X, & Jing L. (2022). Hoxa9/meis1-transgenic zebrafish develops acute myeloid leukaemia-like disease with rapid onset and high penetrance. Open Biology, 12(10), 220172.

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Quantifying Cell Proliferation in Zebrafish

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Embryos were phenotyped at 48 h.p.f. and scored prior to labelling. EdU incorporation was achieved by soaking embryos in 0.2 mM EdU (5-ethynyl-2′-deoxyuridine; Invitrogen), 10% DMSO in E3 medium on ice for 60 min. Following washing (3 × 5 min in E3) and fixation in 4% PFA/PBS for 90 min, embryos were again washed (3 × 5 min in PBST; 1 × dH₂O) and incubated in acetone at −20 °C for 7 min, then permeabilized in 1% DMSO/1% Triton-X100/PBS for 1 h at RT. EdU incorporation was detected by incubation in PBS/0.3% Triton-X100 containing 0.2 mM Alexafluor-555 (Life Technologies), 0.1 M L-ascorbic acid, 100 mM Tris pH 8.5, and 2 mM CuSO₄ for 2 h at RT and washed (5 × 5 min PBST) prior to imaging. Embryos were imaged on an Olympus MVX10 fluorescence microscope and the number of EdU positive cells in caudal haemopoietic tissue counted in Fiji using the Find Maxima algorithm.

Keightley M.C., Carradice D.P., Layton J.E., Pase L., Bertrand J.Y., Wittig J.G., Dakic A., Badrock A.P., Cole N.J., Traver D., Nutt S.L., McCoey J., Buckle A.M., Heath J.K, & Lieschke G.J. (2017). The Pu.1 target gene Zbtb11 regulates neutrophil development through its integrase-like HHCC zinc finger. Nature Communications, 8, 14911.

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Visualizing Arbuscular Mycorrhizal Colonization

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Mycorrhized M. truncatula roots were washed and treated with 10% KOH for 6 min at 95 °C, then placed in ink/acetic acid/water (5:5:90, v/v/v) for 3 min as described by Vierheilig et al.^{58 (link)}. R. irregularis colonisation level was quantified using the grid-line intersect method as described by Giovannetti and Mosse^{59 (link)} and imaged under an Olympus MVX10 fluorescence microscope. Mycorrhized roots were also stained with WGA-Alexa Fluor 488^{6 (link),16 (link)}. In brief, harvested roots were placed in 50% ethanol for at least 4 h and then transferred to 20% (w/v) KOH for 2–3 days, followed by 0.1 M HCl for 1–2 h at room temperature. After HCl was removed, the sample was rinsed twice with distilled H₂O and once with PBS buffer (pH 7.4), then immersed in PBS/WGA-Alexa Fluor 488 staining solution (0.2 μg/mL) in the dark for more than 6 h. Images of WGA-AF488-stained arbuscules were obtained under a Leica SP8 confocal microscope (Germany). GUS staining patterns were observed and photographed with a Zeiss Axio Imager A2 light microscope (Germany).

Zhang Q., Wang S., Xie Q., Xia Y., Lu L., Wang M., Wang G., Long S., Cai Y., Xu L., Wang E, & Jiang Y. (2023). Control of arbuscule development by a transcriptional negative feedback loop in Medicago. Nature Communications, 14, 5743.

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Larval Hemocyte Recruitment Quantification

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Recruitment assays were conducted as described by Kadandale et al., 2010 (link). Briefly, third instar larvae expressing EGFP under the Hml^ΔGal4 driver were damaged at the dorsal face between the A5 and A7 segments using a sharpened tungsten filament. The injury was performed avoiding the location of sessile hemocytes or crowded regions. Larvae were then transferred to agar-apple juice plates; after 6 h, the number of hemocytes recruited to the injury site were quantified and evaluated. For each condition (Control and Prpk-IR), four larvae were wounded and recorded. The experiment was done in quadruplicate. Hemocyte images were taken with an Olympus MVX10 fluorescence microscope, with ×1 objective and ×5 magnification.

Molina E., Cataldo V.F., Eggers C., Muñoz-Madrid V, & Glavic Á. (2022). p53 Related Protein Kinase is Required for Arp2/3-Dependent Actin Dynamics of Hemocytes in Drosophila melanogaster. Frontiers in Cell and Developmental Biology, 10, 859105.

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GFP Silencing in C. elegans

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Male cross progeny were scored for silencing of GFP expressed from nrIs20 (Psur-5::sur-5::gfp) with a fixed magnification on a MVX10 Fluorescence Microscope (Olympus; Fig. 2). Genotypes of scored progeny were confirmed for presence or absence of eri-1 by PCR using primers P7 and P8. Animals with at least one nucleus >10-fold dimmer than the brightest nucleus were scored as silenced, and the proportion of such animals was determined for each genotype (Fig. 2). 95% confidence intervals and p-values for comparison were calculated as described earlier (Jose et al., 2009 (link)).

Le H.H., Looney M., Strauss B., Bloodgood M, & Jose A.M. (2016). Tissue homogeneity requires inhibition of unequal gene silencing during development. The Journal of Cell Biology, 214(3), 319-331.

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Rebaudioside A Influence on C. elegans

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This assay was carried, out as previously described [40 (link)]. L4 stage worms were cultured on NGM plates with or without Reb A (0.0083, 0.017, 0.033, and 0.05 mM), and photos were taken with an Olympus MVX10 fluorescence microscope (Olympus, Beijing, China) on the first day of adulthood. Photos of 50 worms were evaluated from each group, and the experiment was carried out in triplicate.

Li P., Wang Z., Lam S.M, & Shui G. (2021). Rebaudioside A Enhances Resistance to Oxidative Stress and Extends Lifespan and Healthspan in Caenorhabditis elegans. Antioxidants, 10(2), 262.

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RNAi Silencing Optimization and Validation

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For Fig. S2 G and H, forward- and reverse-strand RNA oligos (IDT) against gfp or unc-22 were either injected into one gonad arm of animals at a final concentration of 100 ng/µl individually or after annealing together (cooling at 1°C/min from 95°C to 25°C). The integrity of the injected RNA was checked using nondenaturing polyacrylamide gel electrophoresis. GFP silencing in L4-staged or young adult progeny of each injected worm at 15°C were examined between 4 and 6 d after injection at fixed magnification on a MVX10 fluorescence microscope (Olympus). After scoring for GFP silencing, worms injected with unc-22 dsRNA were scored as silenced if they twitched while suspended in 3 mM tetramisole hydrochloride for at least 30 s.
For Fig. S3 A, the body cavities of L4-staged HC567 animals were injected with either 750 ng/µl in vitro–transcribed gfp-dsRNA (made by J. Marre, University of Maryland, College Park, MD) or 10 mM Tris-HCl, pH 8.5, and the number of brightly fluorescent intestinal nuclei in each injected animal at 15°C was counted after injection at a fixed magnification on a fluorescence microscope (MVX10; Olympus).

Le H.H., Looney M., Strauss B., Bloodgood M, & Jose A.M. (2016). Tissue homogeneity requires inhibition of unequal gene silencing during development. The Journal of Cell Biology, 214(3), 319-331.

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