The HCC cells (Hep3B and Huh7) were obtain from the Cell Bank of the Type Culture Collection of the Chinese Academy of Sciences, Shanghai Institute of Cell Biology, Chinese Academy of Sciences. They were both cultured in high-glucose Dulbecco’s modified Eagle’s medium (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% FBS (Gibco), and 1% penicillin/streptomycin (Hyclone; GE Healthcare Life Sciences, Logan, UT, USA). The HCC cells were cultured in a humidified incubator containing 5% CO2 at 37° C (Thermo Fisher Scientific, Inc.). Abemaciclib (S5716) was purchased from Selleck Chemicals (Houston, TX, USA). A stock solution of Abemaciclib was prepared in dimethyl sulfoxide (DMSO; Sigma, St. Louis, MO, USA) and stored at - 80° C. Abemaciclib was diluted to a final concentration of 10 μM for use in experiments.
Abemaciclib
Manufactured by Merck Group
Sourced in United States
Abemaciclib is a cyclin-dependent kinase (CDK) 4 and 6 inhibitor developed by Eli Lilly and Company. It is used for the treatment of certain types of breast cancer.
Automatically generated - may contain errors
Lab products found in correlation
Abemaciclib, by Selleck Chemicals (3 mentions)
Staurosporine, by Enzo Life Sciences (2 mentions)
Abemaciclib, by Enzo Life Sciences (2 mentions)
Ruxolitinib, by Selleck Chemicals (2 mentions)
Fulvestrant, by Merck Group (2 mentions)
Palbociclib, by Merck Group (2 mentions)
Bathocuproinedisulfonic acid, by Merck Group (1 mentions)
1 thioglycerol, by Merck Group (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by GE Healthcare (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Oasis prime hlb cartridge, by Waters Corporation (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Ammonium acetate, by Merck Group (1 mentions)
5 protocols using abemaciclib
1
Abemaciclib Inhibits HCC Cell Growth
2
Apoptosis Induction in Breast Cancer Cell Lines
3
Evaluation of CDK4/6 Inhibitors in Lymphoma Cell Lines
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The following cell lines were used: Burkitt lymphoma (BL2, 29, 30, 41, 64, 65, 67, 70, 74, Gumbus, Namalwa and Raji), HGBL, DHL (SU‐DHL 4, 6 and 10),12 DLBCL, GCB type (HT, OCI‐Ly7, SU‐DHL 5, 8 and 16) and DLBCL, ABC type (HBL‐1, SUDHL2 and OCI‐Ly10). SU‐DHL 2, 4, 5, 6, 8, 10 and 16 were purchased from ATCC. BL2, 30, 41, 70, Raji, Gumbus, Namalwa and OCI‐Ly10 were obtained from Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ). HBL‐1 was kindly provided by Dr Yuko Hashimoto (Fukushima). BL64, 65, 67, and 74 were generously provided by Dr Georg Bornkamm (München, Germany). BL2, 29, 30, 41, 64, 65, 67 and 74 were maintained in IMDM medium 1640 containing 10% FBS (purchased from PAN Biotech, Aidenbach, Germany or Sigma‐Aldrich Japan), 1% penicillin/streptomycin, 50 μmol/L 1‐thioglycerol (Sigma, M6145) and 20 nmol/L bathocuproinedisulfonic acid (Sigma, B1125) at 37°C under 5% CO2. Other cell lines were maintained in RPMI1640 (Gibco, Life Technologies) containing 10%‐20% heat‐inactivated FBS, 100 U/mL penicillin and 100 μg/mL streptomycin. Two CDK4/6 inhibitors, palbociclib (PD0332991) and abemaciclib (LY2835219), were obtained from Sigma‐Aldrich Japan and AdooQ BioScience, respectively. Both compounds were dissolved in DMSO.
4
Apoptosis Induction in Breast Cancer Cell Lines
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Abemaciclib and lapatinib (Haoyuan Chemexpress) were diluted as previously described6 (link). For in vitro use, palbociclib was diluted in DMSO. Cleaved PARP was measured after 48 hours of treatment with DMSO, lapatinib, or Abemaciclib. Lapatinib was used at 30 nM for BT474 and SKBR3; 500 nM for MDA-MB-453 and MDA-MB-361. Abemaciclib was used at 300 nM for BT474 and SKBR3; 25 nM for MDA-MB-453; 500 nM for MDA-MB-361. For staurosporine experiments, MDA-MB-453 cells were pretreated with DMSO or Abemaciclib (500 nM) for 0, 1, or 7 days prior to exposure to staurosporine (500nM, Enzo Life Sciences) for 4 hours. For hormonal therapy studies, cells were treated with combinations of DMSO, Abemaciclib (100 nM), and fulvestrant (100 nM, Sigma Aldrich). To determine JAK dependency, cells were treated with combinations of DMSO, Abemaciclib (500nM), and ruxolitinib (500nM, Selleckchem) for 7 days.
5
Palbociclib and Abemaciclib LC-MS Assay
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Palbociclib and abemaciclib were purchased from Sigma-Aldrich (Taufkirchen, Germany) and Alsachim (Illkirch Graffenstaden, France), respectively. Ammonium acetate, acetic acid, and MS grade ultrapure LC solvents (water, acetonitrile, methanol) came from Merck (Bucharest, Romania). Oasis PRiME HLB cartridges were purchased from Waters (Romania). Blank human plasma was achieved from our local blood bank (Blood Transfusion Center, Craiova, Romania). Chemical structures of Palbociclib and abemaciclib are presented in Figure 4 .
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