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Tri gas co2 incubator

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The Tri-gas CO2 incubator is a laboratory equipment that maintains a controlled environment for cell and tissue culture applications. It provides precise regulation of temperature, humidity, and carbon dioxide levels to support optimal growth conditions for cells and tissues.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Spectramax m5 microplate reader, by Molecular Devices (1 mentions)

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CO2 incubator (511 citations) Tri-gas incubator (30 citations)

4 protocols using tri gas co2 incubator

1

Modeling Hypoxia-Reoxygenation in C2C12 Cells

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Mouse C2C12 myoblast cells (Shanghai Cellular Institute of China Scientific Academy) were cultured in Dulbecco's modified Eagle's medium (DMEM; Gibco; Thermo Fisher Scientific Inc.) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific Inc.) in 5% CO2 at 37°C. In order to establish the H/R cell model, when cells reached 80% confluence, hypoxia was induced by changing the air content to 0.5% O2, 93% N2 and 5% CO2 gas mixture in a tri-gas CO2 incubator (Thermo Fisher Scientific Inc.) and replacing the media with fresh deoxygenated serum-free high glucose DMEM for 6 h. Subsequently, the medium was replaced with the normal media, and incubated in 95% air and 5% CO2 for 6 h for reoxygenation. The normal group was cultured in normal conditions for the same period of time, respectively. TUNEL staining and western blot, chloroquine (40 nM) was added to media prior to the hypoxia and H/R phase for the regulation of autophagy flux.
Liu C., Peng M., Zheng L., Zhao Y., Wang R., Su Q., Chen S, & Li Z. (2019). Enhanced autophagy alleviates injury during hindlimb ischemia/reperfusion in mice. Experimental and Therapeutic Medicine, 18(3), 1669-1676.
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2

Hypoxic Conditioning of Cancer Cells

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Logarithmically growing cells were harvested and seeded in 6-well sterile plastic culture plates (Corning Inc., Corning, NY, USA) at a density of 1~3 × 104/ml in RPMI-1640 medium supplemented with 10% FBS at 37 °C, and cultured in a tri-gas CO2 incubator (Thermo Fisher, Carlsbad, CA, USA) with a 5% CO2 and 1% O2 atmosphere (hypoxic condition). When the cell density reached 1~3 × 105/ml, various concentrations of Dex (0.10 μM, 0.25 μM and 0.50 μM) were added to the culture plates. After 10~14 days in the lag-phase, the cells began to grow. The medium was replaced every 3~4 days to maintain the cells at a density of 5~10 × 105/ml in the next 2~3 weeks. After 5~6 weeks of culture under the hypoxic condition, the cells were transferred to the normoxic condition and cultured in drug-free medium continuously for 1 year.
Gu L., Zhang G, & Zhang Y. (2019). A novel method to establish glucocorticoid resistant acute lymphoblastic leukemia cell lines. Journal of Experimental & Clinical Cancer Research : CR, 38, 269.
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3

Hypoxia-induced cell viability in HPAECs and HPASMCs

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The HPAECs at passage of 4–6 were seeded into normal 96 well plates at a density of 5,000 cells/well. The HPASMCs at passage of 4–6 were cultured in normal 96 well plates at a density of 4,000 cells/well. After 24 h, the cells were changed to fresh serum-free medium for 24 h. Then, HPAECs and HPASMCs were treated with or without DSY (1, 3 and 10 μg/ml) for 2 h in the normal incubator. The cells in control groups were remained in normal conditions (21% O2, 5% CO2). The others were moved into the Tri-Gas CO2 incubator (Thermo, United States) containing humidified hypoxia gas (1% O2, 5% CO2). After 48 h incubation, the cell viability was detected by the CCK8 kit. Absorbance values were read at the wave of 450 nm by a Spectra Max M5 microplate reader (Molecular Device, San Jose, CA, United States).
Wang R.R., Yuan T.Y., Chen D., Chen Y.C., Sun S.C., Wang S.B., Kong L.L., Fang L.H, & Du G.H. (2022). Dan-Shen-Yin Granules Prevent Hypoxia-Induced Pulmonary Hypertension via STAT3/HIF-1α/VEGF and FAK/AKT Signaling Pathways. Frontiers in Pharmacology, 13, 844400.
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4

Isolation and Culturing of DASMCs

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DASMCs from human neonates were isolated from freshly isolated human ductus arteriosus. The identity of the cells as DASMCs was confirmed by confirming positive immunostaining for SM α-actin but not vonWillebrand’s factor. A primary culture of DASMCs was established and then the cells were harvested with trypsin, frozen in Freezing Media (10% DMSO, 50% FBS and 40% DMEM) and stored in liquid nitrogen for later use. DASMC were grown in 100 mm culture dishes and used within the first 5 passages in culture. DASMC were maintained in hypoxia (pO2 40 mmHg, pH 7.35–7.45, pCO2 30–40 mmHg using an environmentally controlled, Tri-Gas CO2 incubator (Thermo Scientific) until the protocol called for exposure to normoxia.
Hong Z., Cabrera J.A., Mahapatra S., Kutty S., Weir E.K, & Archer S.L. (2014). Activation of the EGFR/p38/JNK Pathway by Mitochondrial-Derived Hydrogen Peroxide Contributes To Oxygen-induced Contraction Of Ductus Arteriosus. Journal of molecular medicine (Berlin, Germany), 92(9), 995-1007.
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