Szx16 stereomicroscope

Elutriation was performed in an Avanti J-26S XP elutriator equipped with a JE 5.0 rotor and a standard 40 ml large elutriation chamber (Beckman Coulter Inc., California, United States). A variable-speed pump (BT100-1L equipped with a YZII15 pump head, Baoding Longer Peristaltic Pump Co., Ltd., Hebei, China), a bubble trap, a manometer for monitoring back pressure in the rotor, and tubing with three-way valves were employed to route the cell suspension into the chamber (Figure 1).
The optimized protocol for counter-flow centrifugal elutriation of synchronize cells is shown in Supplementary Table 1. Cell integrity was monitored by light microscopy (Olympus SZX16 stereomicroscope, Olympus Co., Japan) at 100 × magnification. Fractions were collected from the elutriation system and nuclei were extracted immediately and stored at −20°C for flow cytometry analysis.

Some interesting specimens belonging to the genus Phryna, in which their main distribution area is the Irano–Turanian phytogeographical region, were collected from Malatya province. As a result of examining the literature (Barkoudah 1962 , Huber-Morath 1967 ) and herbarium specimens, it was found that the collected specimens resemble Phrynaortegioides but differ from this species in terms of calyx length, calyx teeth length, petal colour, petal calyx ratio and capsule calyx ratio. Flower, petal and capsule pictures were taken with an OLYMPUS SZX-16 Stereomicroscope, DP 72 digital camera and seed surface images were taken by a Quanta Feg 450 scanning electron microscope (SEM) at Bozok University Research and Application Centre. The vegetative parts were measured with a ruler with 0.5 mm accuracy and the floral characteristics were studied using an ocular micrometer.

Isolates on PDA plates were incubated at 25 °C for 30 days under near-ultraviolet (UV) light (12 h light/12 h dark). The growth rate of mycelium was measured in five duplicates. Colony color on PDA, Corn meal agar (CMA), and Oatmeal agar (OMA) media incubated at 25 °C near UV light with 12 h, was investigated according to the method of Rayner [84 ]. The morphology imagines were taken using Canon 600D digital camera (Canon Inc., Tokyo, Japan) after 10 days of incubation. Conidiomata and conidia were observed under the OLYMPUS SZX16 stereomicroscope (Olympus Corporation, Tokyo, Japan), conidial length/wide ratio of 30 conidia was measured with a stage micrometer under a Motic BA200 light microscope (Motic China Group Co., Ltd., Nanjing, China). Alpha and beta conidia were measured for calculating means ( $\overline{x}$ ) and standard deviations (SD). The conidia ranges were shown as (min−) $\overline{x}$ − SD − $\overline{x}$ + SD (−max) μm ( $\overline{x}$ ± SD). Conidia digital images were captured using Nikon Eclipse 80i compound light microscope imaging system (Nikon Corporation, Tokyo, Japan).

Tg (vegfr2:GFP) zebrafish embryos 24 h post-fertilization were treated with 1 mg/mL pronase E solution to remove the egg membrane. Then, they were randomly divided into 4 groups, namely the normal control group, the model group (vatalanib, PTK787), the positive control group (Danhong injection), and the experimental group. Each group had 10 zebrafish embryos, and each group had two parallel repeats.
As shown in Figure 8A, the model group was built successfully based on significant inhibition of the growth of intersegmental blood vessels (ISVs) by treating zebrafish embryos with vatalanib (PTK787). Then, the positive control group and experimental group were also treated with vatalanib PTK787 (0.2 μg/mL) to afford model zebrafish whose intersegmental blood vessels (ISVs) were significantly inhibited. Simultaneously, the positive control group was given a Danhong injection (10 μL/mL) and the experimental group was given various concentrations of tested compounds (20 μM, 40 μM, 80 μM).
After incubation at 28 °C for 24 h, the intersegmental blood vessels (ISVs) of zebrafish were observed and visualized under a fluorescence microscope (SZX16 stereo microscope and DP2-BSW image acquisition system, Olympus, Japan), and the ISV index was calculated as follows: ISV index = number of intact vessels × 1 + number of defective vessels × 0.5 [12 (link)].