Log In Sign up
The largest database of trusted experimental protocols

10 day embryonated chicken eggs

Manufactured by Charles River Laboratories
Visit Supplier

10-day embryonated chicken eggs are a laboratory product used for various scientific and research purposes. They provide a biological matrix for the study of embryonic development, cell culture, and other applications that require an avian embryonic system.

Automatically generated - may contain errors

Lab products found in correlation

Embryonated chicken eggs, by Charles River Laboratories (1 mentions)

Spelling variants of this product

Embryonated chicken eggs (37 citations) Chicken eggs (6 citations) Embryonic day 10 (E10) White Leghorn embryonated chicken eggs (3 citations) SPF 10-day-old chicken embryonated eggs (2 citations)

2 protocols using 10 day embryonated chicken eggs

1

Influenza Virus Strains Propagation and Titration

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Influenza virus strains A/Puerto Rico/8/34 (H1N1) (PR8, provided by Dr. Thomas Moran of the Icahn School of Medicine at Mt. Sinai) and A/Victoria/361/2011 (H3N2, provided by BEI Resources, NR-44042) were propagated in 10-day embryonated chicken eggs (Charles River Laboratories) and titered as previously described66 (link). Stocks of PR8-Cre39 (link) grown in MDCK cells were kindly provided by Dr. Ryan Langlois (University of Minnesota). All viruses were titered on MDCK cells.
Speaks S., McFadden M.I., Zani A., Solstad A., Leumi S., Roettger J.E., Kenney A.D., Bone H., Zhang L., Denz P.J., Eddy A.C., Amer A.O., Robinson R.T., Cai C., Ma J., Hemann E.A., Forero A, & Yount J.S. (2024). Gasdermin D promotes influenza virus-induced mortality through neutrophil amplification of inflammation. Nature Communications, 15, 2751.
+ Open protocol
+ Expand
2

Influenza Virus Propagation and Titration

Check if the same lab product or an alternative is used in the 5 most similar protocols
Influenza viruses A/Puerto Rico/8/34 (H1N1) (PR8, provided by Dr. Thomas Moran of the Icahn School of Medicine at Mt. Sinai), A/Victoria/361/2011 (H3N2) (BEI Resources), and A/California/4/2009 (H1N1) (BEI Resources) were propagated in 10-day embryonated chicken eggs (Charles River Laboratories) and titered on MDCK cells. The potential zoonotic viruses were collected by Andrew Bowman of the Ohio State University and were also propagated in embryonated chicken eggs (Charles River Laboratories) and titered on MDCK cells. Infection of cell lines was done at an MOI of 1 for 24hrs. Cells that received Interferon-β pre-treatment were stimulated for 18 hours with 100 units of recombinant human Interferon-β protein (Millipore, IF014) prior to infection.
Denz P.J., Speaks S., Kenney A.D., Eddy A.C., Papa J.L., Roettger J., Scace S.C., Hemann E.A., Forero A., Webby R.J., Bowman A.S, & Yount J.S. (2023). Innate immune control of influenza virus interspecies adaptation. bioRxiv.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!