Anti human cd68

Manufactured by Proteintech

Sourced in United States

Anti-human CD68 is a primary antibody used in laboratory research. CD68 is a glycoprotein expressed by cells of the monocyte-macrophage lineage, and this antibody can be used to detect and study these cell types. The antibody is designed for use in various immunoassay applications.

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Lab products found in correlation

Opal 7 color ihc kit, by Akoya Biosciences (1 mentions) Lsm 880 confocal laser scanning microscope, by Zeiss (1 mentions) Zen image analysis software, by Zeiss (1 mentions) Anti mouse f4 80, by Thermo Fisher Scientific (1 mentions) 3 3 diaminobenzidine (dab), by Vector Laboratories (1 mentions) Anti human nox1, by GeneTex (1 mentions)

Spelling variants of this product

Anti-CD68 (15 citations) Anti-TM (2 citations)

2 protocols using anti human cd68

Multiplex Immunostaining of FFPE CRC Tissues

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Formalin‐fixed and paraffin‐embedded (FFPE) tissue blocks were chosen from nine CRC patients in Nanfang Hospital for subsequent analysis. Immunostaining was processed with Opal™ 7‐Color IHC Kits (Akoya Biosciences, Marlborough, MA, USA; NEL821001KT), according to the manufacturer's instructions using antibodies (anti‐human CD68 (Proteintech, Chicago, IL, USA; Cat# 66231‐2‐Ig, RRID: AB_2881622); anti‐human APOE (Proteintech; Cat# 66830‐1‐Ig, RRID: AB_2882173); anti‐human CTSZ (Affinity Biosciences, Melbourne, VIC, Australia; Cat# DF14386, RRID:AB_2923130); anti‐human GLUL (Affinity Biosciences; Cat# DF7607, RRID: AB_2841098), and anti‐human FOXP3 (Affinity Biosciences; Cat# AF6544, RRID: AB_2847268)). Images were obtained and analyzed with the Zeiss LSM 880 confocal laser‐scanning microscope (LSM 880; Carl Zeiss, Oberkochen, Germany) and ZEN image analysis software (zen; Carl Zeiss). imagej was used to perform the fluorescence quantitative colocalization analysis [29 (link)].

Wei J., Yu W., Chen J., Huang G., Zhang L., Chen Z., Hu M., Gong X, & Du H. (2023). Single‐cell and spatial analyses reveal the association between gene expression of glutamine synthetase with the immunosuppressive phenotype of APOE+CTSZ+TAM in cancers. Molecular Oncology, 17(4), 611-628.

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Immunohistochemical Analysis of Liver Samples

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Formalin-fixed, paraffin-embedded human or mouse livers were stained with Haemotoxylin and Eosin (H&E). Human livers were immunostained with anti-human NOX1 (GeneTex, GTX103888); antihuman CD68 (Proteintech, 66231–1-Ig). Mouse livers were stained with anti-mouse NOX1 (Abcam, ab55831): anti-mouse AFP (Biocare, SKU028); anti-mouse YAP-1 (Cell Signaling, CST8418); anti-mouse Ki67 (Genetex, GTX16667); anti-mouse Desmin (Thermo Fisher Scientific, RB-9014-P0); and anti-mouse F4/80 (eBioscience, 14–4801-82) Abs, followed by staining with DAB (Vector Laboratories) or with secondary Alexa Fluor 594 or Alexa Flour 488 conjugated Abs. Images were taken using an Olympus microscope, and positive areas were calculated using ImageJ software (NIH).

Liang S., Ma H.Y., Zhong Z., Dhar D., Liu X., Xu J., Koyama Y., Nishio T., Karin D., Karin G., Mccubbin R., Zhang C., Hu R., Yang G., Chen L., Ganguly S., Lan T., Karin M., Kisseleva T, & Brenner D.A. (2018). NADPH Oxidase 1 in Liver Macrophages Promotes Inflammation and Tumor Development in Mice. Gastroenterology, 156(4), 1156-1172.e6.

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