Ammonium formate

Manufactured by Hampton Research

Ammonium formate is a chemical compound that serves as a buffer solution. It is commonly used in analytical and research applications to maintain a specific pH level in various samples or experimental conditions.

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Lab products found in correlation

2 protocols using ammonium formate

Quantitative Analysis of Vitamins and Compounds

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Thiamin hydrochloride, thiamin-monophosphate, thiamin-pyrophosphate, riboflavin, flavin adenine dinucleotide, riboflavin-dioxopyrimidine-¹³C₄,¹⁵N₂, perchloric acid, phosphoric acid and sodium hydroxide were purchased from Sigma-Aldrich (St. Louis, MO). Methanol, acetonitrile, water (all LC-MS grade), phosphoric acid and potassium phosphate dibasic were obtained from Fisher Scientific (Fair Lawn, NJ). Potassium ferricyanide (III) was purchased from Acros Organics (Geel, Belgium); caffeine-trimethyl-¹³C₃ from Cambridge Isotope Laboratories (Andover, MA) and ammonium formate from Hampton Research (Aliso Viejo, CA). HPLC screw-cap amber vials and inserts were obtained from Supelco (Bellefonte, PA). LC-vial caps (PTFE/silicone) were purchased from Waters (Milford, MA) and Agilent Technologies (Santa Clara, CA), and ultrafree centrifugal filters Durapore® PVDF 0.1μm from Millipore (Billerica, MA).

Hampel D., Shahab-Ferdows S., Adair L.S., Bentley M.E., Flax V.L., Jamieson D.J., Ellington S.R., Tegha G., Chasela C.S., Kamwendo D, & Allen L.H. (2016). Thiamin and Riboflavin in Human Milk: Effects of Lipid-Based Nutrient Supplementation and Stage of Lactation on Vitamer Secretion and Contributions to Total Vitamin Content. PLoS ONE, 11(2), e0149479.

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Quantification of Amatoxins in Urine

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α-, β-, and γ-amanitin (1 mg dried each, ≥90% purity) were purchased from Enzo Biochem, Inc. (Farmingdale, NY). ¹⁵N₁₀-labeled α-amanitin was acquired from Michigan State University (East Lansing, MI) [Luo, 2015 (link)]. Synthetic peptide with the methionine sulfoxide (MetSSP) moiety was purchased from Battelle (Columbus, OH). Ammonium formate (10 M, crystallization grade) was purchased from Hampton Research (Aliso Viejo, CA). Formic acid (LC/MS grade, ≥ 99.5%), methanol (LC/MS grade, ≥ 99.9%), and acetonitrile (HPLC grade, ≥ 99.9%) were purchased from Fisher Scientific (Waltham, MA). Deionized (DI) water (18 MΩ-cm) was obtained from a filter system manufactured by AquaSolutions (Jasper, GA). Pooled urine and a convenient set of individual, unexposed urine samples were purchased from Tennessee Blood Services (Memphis, TN). All urine was pre-screened by the vendor in accordance with FDA regulations to be free of Hepatitis B, Syphilis, and HIV. This study used de-identified urine acquired from commercial sources, and thus the work did not meet the definition of human subjects as specified in 45 CFR 46.102 (f).

Abbott N.L., Hill K.L., Garrett A., Carter M.D., Hamelin E.I, & Johnson R.C. (2018). Detection of α-, β-, and γ-amanitin in urine by LC-MS/MS using 15N10-α-amanitin as the internal standard. Toxicon : official journal of the International Society on Toxinology, 152, 71-77.

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