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Mouse α gapdh

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Mouse α-GAPDH is a protein that functions as a glyceraldehyde-3-phosphate dehydrogenase, an enzyme involved in the glycolytic pathway. It catalyzes the oxidative phosphorylation of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate.

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Lab products found in correlation

Rabbit α gapdh, by Cell Signaling Technology (2 mentions) Pou4f1, by Santa Cruz Biotechnology (2 mentions) Geldoc imager, by Jackson ImmunoResearch (2 mentions) Foxc1, by Cell Signaling Technology (2 mentions) Mouse anti rabbit hrp, by Santa Cruz Biotechnology (2 mentions) Goat anti mouse hrp, by Jackson ImmunoResearch (2 mentions) Secondary horseradish peroxidase coupled antibodies, by Santa Cruz Biotechnology (1 mentions) Rabbit α p65, by Cell Signaling Technology (1 mentions) Mouse α gfp, by Santa Cruz Biotechnology (1 mentions) Goat α mouse hrp, by Jackson ImmunoResearch (1 mentions) Goat α rabbit hrp, by Jackson ImmunoResearch (1 mentions) Rat α ha, by Roche (1 mentions) Rabbit α h3k9me3, by Abcam (1 mentions) Rabbit α h3, by Abcam (1 mentions) Mouse α actin, by Merck Group (1 mentions)

5 protocols using mouse α gapdh

1

Western Blot Analysis for Knockdown Efficiency

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Western blot analysis was performed using 10% SDS polyacrylamide gels and polyvinylidene difluoride blotting membranes (Merck). To analyse knockdown efficiencies, the following antibodies were used: rabbit α-GOT1 (14886-1-AP, Proteintech, Rosemont, IL, USA), rabbit α-GOT2 (14800-1-AP, Proteintech), rabbit α-STAT3 (9132, Cell Signaling Technology, Danvers, MA, USA), rabbit α-p65 (4764, Cell Signaling Technology), and mouse α-GAPDH (ab8245, Abcam, Cambridge, UK). For visualization of the bound antibodies, secondary horseradish peroxidase-coupled antibodies from Santa Cruz Biotechnology (Dallas, TX, USA) were employed (sc-2004 and sc-2005). Uncropped versions of all blots can be found at the end of the Supplementary Information (Supplementary Fig. 13-19).
Feist M., Schwarzfischer P., Heinrich P., Sun X., Kemper J., von Bonin F., Perez-Rubio P., Taruttis F., Rehberg T., Dettmer K., Gronwald W., Reinders J., Engelmann J.C., Dudek J., Klapper W., Trümper L., Spang R., Oefner P.J, & Kube D. (2018). Cooperative STAT/NF-κB signaling regulates lymphoma metabolic reprogramming and aberrant GOT2 expression. Nature Communications, 9, 1514.
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2

Western Blot Analysis of Regulatory Proteins

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Protein lysates from cell lines were analyzed by Western blot. Relevant primary antibodies against FOXC1 (Cell Signaling Technology - #8758), NFIX (Invitrogen - #PA5-31234), POU4F1 (Santa Cruz Biotechnology – sc-8429) were used to detect target genes and GAPDH (mouse αGAPDH – Abcam – ab8245; rabbit αGAPDH – Cell Signaling Technology – 2118L) was used as a housekeeping gene. Secondary antibodies mouse anti-rabbit HRP (Santa Cruz Biotechnology – sc-2054) and goat anti-mouse HRP (Jackson ImmunoResearch – 115-035-062) enabled detection and quantifications by densitometry using Imagelab software and a GelDoc imager.
Assi S.A., Imperato M.R., Coleman D.J., Pickin A., Potluri S., Ptasinska A., Chin P.S., Blair H., Cauchy P., James S.R., Zacarias-Cabeza J., Gilding L.N., Beggs A., Clokie S., Loke J.C., Jenkin P., Uddin A., Delwel R., Richards S.J., Raghavan M., Griffiths M.J., Heidenreich O., Cockerill P.N, & Bonifer C. (2018). Subtype-specific regulatory network rewiring in acute myeloid leukemia. Nature genetics, 51(1), 151-162.
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3

Chromatin Immunoprecipitation and Western Blot Antibodies

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The following antibodies were used for ChIP: rabbit α-H3K9me3 (Abcam ab8898), rabbit α-H3K36me3 (Abcam ab9050), mouse α-H3K9me2 (Millipore 05-1249), rabbit α-H3 (Abcam ab1719) and mouse α-IgG (Sigma I5381).
For Western blot the following antibodies were used: mouse α-GAPDH (Abcam ab9484), rabbit α-mRFP (Apronex), rat α-HA (Roche), mouse α-GFP (Santa Cruz sc-9996), rabbit α-H3K9me3 (Abcam ab8898), mouse α-tubulin kindly provided by Pavel Draber (Institute of Molecular Genetics of the ASCR, Prague, Czech Republic), goat α-mouse-HRP (Jackson ImmunoResearch), goat α-rabbit-HRP (Jackson ImmunoResearch), goat α-rat-HRP (Santa Cruz sc-2006).
Bieberstein N.I., Kozáková E., Huranová M., Thakur P.K., Krchňáková Z., Krausová M., Oesterreich F.C, & Staněk D. (2016). TALE-directed local modulation of H3K9 methylation shapes exon recognition. Scientific Reports, 6, 29961.
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4

Western Blotting and Immunofluorescence Staining

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The following antibodies were used for western blotting: mouse-α-CD9 (1:2000, clone HI9a; Biolegend, San Diego, CA), mouse-α-Flotillin-1 (1:1000, clone 18/Flotillin-1; BD Biosciences), mouse-α-LC3 (1:500, clone 5F10, Nanotools, Teningen, Germany), rabbit-α-LC3 (1:1000, polyclonal; MBL international, Woburn, MA), rabbit-α-phospho P38 MAPK (1:2000, polyclonal, Promega), mouse-α-HSP90 (1:1000, clone 68/HSP90, BD Biosciences), rabbit-α-phospho PKR (1:1000, clone E120; Abcam), mouse-α-actin (1:30,000, clone AC-15, Sigma-Aldrich), mouse-α-GAPDH (1:2000, clone mAbcam 9484; Abcam), HRP-coupled goat-α-mouse secondary antibody (1:10,000; Jackson ImmunoResearch Labaratories Inc., West Grove, PA), goat-α-rabbit secondary antibody (1:2500, P0448, DAKO, Denmark). For immunofluorescence staining cells were stained with rabbit-α-G3BP1 (1:150, polyclonal, Aviva, San Diego, CA), mouse-α-hnRNPK (1:100, clone D-6, Santa Cruz), goat-α-mouse or goat-α-rabbit Alexa488 and 647 (1:200, polyclonal, Invitrogen, MA), donkey-α-mouse Alexa488 and 647 (1:200, polyclonal, Thermo Fischer Scientific).
Defourny K.A., Pei X., van Kuppeveld F.J, & Nolte-´t Hoen E.N. (2024). Picornavirus security proteins promote the release of extracellular vesicle enclosed viruses via the modulation of host kinases. PLOS Pathogens, 20(4), e1012133.
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5

Western Blot Analysis of Regulatory Proteins

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Protein lysates from cell lines were analyzed by Western blot. Relevant primary antibodies against FOXC1 (Cell Signaling Technology - #8758), NFIX (Invitrogen - #PA5-31234), POU4F1 (Santa Cruz Biotechnology – sc-8429) were used to detect target genes and GAPDH (mouse αGAPDH – Abcam – ab8245; rabbit αGAPDH – Cell Signaling Technology – 2118L) was used as a housekeeping gene. Secondary antibodies mouse anti-rabbit HRP (Santa Cruz Biotechnology – sc-2054) and goat anti-mouse HRP (Jackson ImmunoResearch – 115-035-062) enabled detection and quantifications by densitometry using Imagelab software and a GelDoc imager.
Assi S.A., Imperato M.R., Coleman D.J., Pickin A., Potluri S., Ptasinska A., Chin P.S., Blair H., Cauchy P., James S.R., Zacarias-Cabeza J., Gilding L.N., Beggs A., Clokie S., Loke J.C., Jenkin P., Uddin A., Delwel R., Richards S.J., Raghavan M., Griffiths M.J., Heidenreich O., Cockerill P.N, & Bonifer C. (2018). Subtype-specific regulatory network rewiring in acute myeloid leukemia. Nature genetics, 51(1), 151-162.
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