Dulbecco's modified Eagle's medium (DMEM) and other culture reagents were obtained from Gibco Life Technologies (Grand Island, NY).The cell culture plates were purchased from Nalge Nunc International (Roskilde, Denmark). Human insulin (HumulinR) was from Eli Lilly S.A.S.(Fegersheim, France). Bovine serum albumin (BSA), forskolin, IBMX, and dexamethasone were purchased from Sigma (St Louis, MO, USA). Compound C was purchased from Calbiochem (San Diego, CA). Anti-CREB, anti-phospho-CREB (Ser133), anti-PPARγ, anti-C/EBPα, anti-fatty acid synthase (FAS), anti-fatty acid binding protein 4 (FABP4), anti-C/EBPβ, anti-AMPK, anti-phospho-AMPK (Thr172), anti-acetyl-CoA carboxylase (ACC), anti-phospho-ACC(Ser79), anti-β-actin, anti-α1-tubulin, anti-mouse IgG and anti-rabbit IgG conjugated with horseradish peroxidase were from Cell Signaling Technology (Beverly, MA, USA). Murine-derived 3T3-L1 preadipocytes were purchased from American Type Culture Collection (Rockville, MD). Berberine was obtained from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China).
Anti acetyl coa carboxylase
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-acetyl-CoA carboxylase (ACC) is a primary enzyme involved in the regulation of fatty acid synthesis. It catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, which is the rate-limiting step in fatty acid biosynthesis.
Automatically generated - may contain errors
Lab products found in correlation
Anti phospho acc, by Cell Signaling Technology (5 mentions)
Anti ampk, by Cell Signaling Technology (5 mentions)
Anti fatty acid synthase, by Cell Signaling Technology (4 mentions)
Anti phospho ampk thr172, by Cell Signaling Technology (3 mentions)
Anti phospho ampk, by Cell Signaling Technology (3 mentions)
Anti phospho akt, by Cell Signaling Technology (3 mentions)
Anti phospho 4ebp1, by Cell Signaling Technology (3 mentions)
Anti phospho acetyl coa carboxylase, by Cell Signaling Technology (3 mentions)
Anti phospho acc ser79, by Cell Signaling Technology (2 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (2 mentions)
Anti β actin, by Cell Signaling Technology (2 mentions)
Anti caspase 3, by Cell Signaling Technology (2 mentions)
Anti phospho acetyl coa carboxylase p acc, by Cell Signaling Technology (2 mentions)
Anti actin, by Applied Biological Materials (2 mentions)
Las4000 system, by GE Healthcare (2 mentions)
Anti ampkα1, by Cell Signaling Technology (2 mentions)
Anti bcl 2, by Cell Signaling Technology (2 mentions)
Anti phospho mtor, by Cell Signaling Technology (2 mentions)
Anti phospho ampkα, by Cell Signaling Technology (2 mentions)
Anti akt, by Cell Signaling Technology (2 mentions)
18 protocols using anti acetyl coa carboxylase
1
Berberine's Effects on Adipocyte Differentiation
2
Western Blot Analysis of Signaling Proteins
3
Biochemical Assays for Cardiac Injury
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Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were purchased from Gibco-BRL (Grand Island, NY, USA). The kits used for the determination of serum lactate dehydrogenase (LDH) and creatine kinase isoenzyme-MB (CK-MB) content were obtained from Jiancheng Bioengineering Institute (Nanjing, China). MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide], TTC (2,3,5-triphenyltetrazolium chloride), Evans blue, and compound C [AMP-activated protein kinase (AMPK) inhibitor] were purchased from Sigma-Aldrich (St. Louis, MO, USA). The primary antibodies were as follows: anti-AMPKα (Thr172; #2532), anti-phosphorylated AMPKα (p-AMPKα) (Thr172; #2531), anti-caspase-3 (#9662), anti-Bcl-2 associated X protein (Bax; #2772), anti-cytochrome c (#11940), anti-acetyl CoA carboxylase (ACC; #3676), anti-p-ACC (#11818), β-actin (#4970), and anti-Bcl-2 (#2870; all from Cell Signaling Technologies, Beverly, MA, USA). All these are rabbit monoclonal antibodies. The secondary antibody (anti-rabbit IgG-B; sc-53804) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The caspase-3 assay kit was purchased from Chemicon International, Inc. (Temecula, CA, USA). The fluorescent kit for Hoechst 33258 was obtained from Roche Diagnostics (Mannheim, Germany).
4
Phospho-AMPK Thr 172 Quantification
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Slides were prepared from isolated pancreata and visualized as previously detailed (11 (link)). ImageJ software was used to calculate the mean intensity on phospho-AMPK Thr 172 in the β-cell area and in the acinar tissue surrounding the islets. Western blotting was performed as previously described (11 (link)) with 100–150 human or mouse islets. Anti–acetyl-CoA-carboxylase (ACC), anti-phospho-ACC, anti-AMPK, anti–phospho-AMPK Thr 172, anti-Raptor, and anti–phospho-Raptor were from Cell Signaling Technology (Danvers, MA, USA). Anti-glucagon was from Sigma-Aldrich (St. Louis, MO, USA).
5
Western Blot Analysis of PVAT Proteins
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Samples of thoracic aortic PVAT were dissected, weighed and lysed as previously described (Almabrouk et al., 2017 (link)). The protein content of WT and KO PVAT lysates derived from mice fed either ND or HFD was calculated by Coomassie Plus Protein Assay Reagent (Perbio, USA) against a BSA standard curve. Protein samples were resolved by SDS-PAGE, transferred to nitrocellulose membranes and incubated overnight at 4°C with mouse anti-GAPDH (Ambion AM4300) antibodies, or rabbit anti-AMPKα (Cell Signaling Technology #2603), anti-phospho-AMPK Thr172 (Cell Signaling Technology #2535), anti-acetyl CoA carboxylase (ACC) (Cell Signaling Technology #3676) and anti-phospho-ACC Ser79 (Cell Signaling Technology #3661) antibodies. All primary antibodies were diluted 1:1000 in 50% (v/v) TBS, 50% (v/v) Odyssey®-Block (LI-COR, USA). Immunolabelled proteins were visualized using infrared dye-labeled secondary antibodies and an Odyssey Sa Infrared Imaging System (LI-COR, USA).
6
Western Blot Analysis of Cellular Proteins
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For Western blot analysis, polypeptides in whole cell lysates were resolved by SDS-PAGE and transferred to nitrocellulose membrane filters. Proteins were detected with a 1:1000 or 1:5000 dilution of primary antibody using an enhanced chemiluminescence (ECL) system. Images were acquired using the Chemidoc-it 410 imaging system (UVP, Upland, CA, USA) and LAS4000 system (GE Healthcare, Uppsala, Sweden). The following primary antibodies were used: anti-AMPK α1 (Cell Signaling Technology, Beverly, MA, USA), anti-phospho-AMPK α1 (Cell Signaling Technology), anti-LC3 (MBL international, Watertown, MA, USA), anti-p62 (MBL international) , anti-acetyl-CoA carboxylase (ACC) (Cell Signaling Technology), anti-phospho acetyl-CoA carboxylase (p-ACC) (Cell Signaling Technology), and anti-actin (ABM, Richmond, BC, Canada).
7
Mechanistic Insights into KL1333 Action
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KL1333 was synthesized as a derivative of β-lapachone. Recombinant human NQO1 (rhNQO1) protein, NAD, NADH, bovine serum albumin (BSA), Coenzyme Q10 (CoQ10), idebenone, and cytochrome c were purchased from Sigma-Aldrich. CM-H2DCFDA, MitoTracker Green FM, and tetramethylrhodamine methyl ester (TMRM) were purchased from Invitrogen. FLAG-tagged mouse PGC-1α plasmid was purchased from Origen. Nitrocellulose membrane and the Enhanced Chemiluminescence (ECL) system were purchased from Amersham. Anti-AMPK, anti-acetyl-CoA carboxylase (ACC), anti-phospho-AMPK, anti-phospho-ACC, and anti-phospho-serine antibodies were purchased from Cell Signaling Technology. Anti-FLAG and anti-actin antibodies were purchased from Sigma-Aldrich. Anti-NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit A9 (NDUFA9), anti-ubiquinol-cytochrome c reductase core protein 1 (UQCRC1), and anti-ATP synthase subunit alpha (ATP5A) antibodies were purchased from Abcam. Anti-succinate dehydrogenase A (SDHA) and anti-cytochrome c oxidase I (COX I) antibodies were purchased from Invitrogen.
8
Branched Polyethylenimine-based Nanoparticle Synthesis
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Branched
polyethylenimine (PEI, MW ∼25000
Da), tetrabutylammonium chloride (TBA, 98%), α-lipoic acid (LA,
99%), 4-(dimethylamino)-pyridine (DMAP, 98%), streptomycin, penicillin,
dicyclohexylcarbodiimide (DCC, 98%), sodium borohydride (NaBH4, 98%),
and palmitic acid were obtained from Sigma-Aldrich Chemical Co. Methylthiazolyldiphenyl-tetrazolium
bromide (MTT, 98%) was purchased from Energy & Chemical Co., Ltd.
Renilla luciferase assay kits and dual-luciferase reporter assay kits
were purchased from Promega. The triglyceride and cholesterol assay
kits were gained from Applygen Technologies Inc., Beijing, China.
TRIzol and lipofectamine 3000 transfection reagent were purchased
from Invitrogen.
Anti-SREBP-1c antibody (#14088-1-AP) was purchased
from Proteintech. Anti-MST1 (#3682), anti-AMPK (#5831), anti-phospho-AMPK
(#2535), anti-phospho-SREBP-1c (#9874), anti-Acetyl-CoA carboxylase
(ACC) (#3662), and anti-phospho-ACC (#3661) antibodies were obtained
from Cell Signaling Technology. Anti-FAS antibody (#Ab22759) was purchased
from Abcam (Cambridge, UK).
polyethylenimine (PEI, MW ∼25000
Da), tetrabutylammonium chloride (TBA, 98%), α-lipoic acid (LA,
99%), 4-(dimethylamino)-pyridine (DMAP, 98%), streptomycin, penicillin,
dicyclohexylcarbodiimide (DCC, 98%), sodium borohydride (NaBH4, 98%),
and palmitic acid were obtained from Sigma-Aldrich Chemical Co. Methylthiazolyldiphenyl-tetrazolium
bromide (MTT, 98%) was purchased from Energy & Chemical Co., Ltd.
Renilla luciferase assay kits and dual-luciferase reporter assay kits
were purchased from Promega. The triglyceride and cholesterol assay
kits were gained from Applygen Technologies Inc., Beijing, China.
TRIzol and lipofectamine 3000 transfection reagent were purchased
from Invitrogen.
Anti-SREBP-1c antibody (#14088-1-AP) was purchased
from Proteintech. Anti-MST1 (#3682), anti-AMPK (#5831), anti-phospho-AMPK
(#2535), anti-phospho-SREBP-1c (#9874), anti-Acetyl-CoA carboxylase
(ACC) (#3662), and anti-phospho-ACC (#3661) antibodies were obtained
from Cell Signaling Technology. Anti-FAS antibody (#Ab22759) was purchased
from Abcam (Cambridge, UK).
9
Western Blot Analysis of Tumor Tissue
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Western blot analysis was carried out according to standard procedures using enhanced-chemiluminescence detection (GE Life Science, Chicago, IL, USA). Tumor tissue lysates were prepared using an electric homogenizer for 30 seconds after the addition of lysis buffer. The following antibodies were used: anti-β-actin, anti-acetyl-CoA carboxylase (ACC), anti-phospho-ACC, anti-phospho-AKT, anti-phospho-BRCA1, anti-caspase-3, anti-caspase-7, anti-β-catenin, anti-phospho-cyclin D1, anti-phospho-GSKα/β, anti-MAPK, anti-phospho-MAPK, anti-PARP, anti-PDK1, anti-phospho-PDK1, anti-PI3K, anti-phospho-PI3K, anti-phospho-S6, anti-phospho-mTOR, anti-phospho-Rb, anti-VEGFR, anti-phospho-VEGFR (all from Cell Signaling Technology, Danvers, MA, USA); anti-β-actin, anti-AKT, anti-AKT1, anti-BRCA1, anti-cyclin D1, anti-Rb, anti-S6, anti-mTOR, anti-α-tubulin (all from Santa Cruz, Dallas, TX, USA); and anti-PCNA (Atlas Antibodies, Bromma, Sweden). Horseradish peroxidase-conjugated goat anti-rabbit or anti-mouse antibodies (Jackson Immuno Research, West Grove, PA, USA) were used as secondary antibodies as appropriate.
10
Western Blot Analysis of Cellular Proteins
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For western blot analysis, polypeptides in whole cell lysates were resolved by SDS-PAGE and transferred to PVDF membrane filters. Proteins were detected with a 1:1000 or 1:5000 dilution of primary antibody using an enhanced chemiluminescence (ECL) system. Images were acquired using the LAS4000 system (GE Healthcare, Uppsala, Sweden) and Chemidoc-it 410 imaging system (UVP, Upland, CA, USA). The following primary antibodies were used: anti-telomerase (Abcam, Cambridge, UK, ab32020), anti-AMPKα1 (Cell Signaling Technology, Beverly, MA, USA, #2532), anti-acetyl-CoA carboxylase (ACC) (Cell Signaling Technology, #3676), anti-phospho acetyl-CoA carboxylase (p-ACC) (Cell Signaling Technology, #3661), anti-c-Myc (Santa Cruz Biotechnology, Santa Cruz, CA, USA, sc788) and anti-actin (ABM, Richmond, BC, Canada, G043).
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