Goat anti rabbit igg h l hrp

Manufactured by Bioworld Technology

Sourced in United States, China

Goat anti-rabbit IgG (H+L)-HRP is a secondary antibody conjugated with horseradish peroxidase (HRP). It is designed to detect and bind to rabbit primary antibodies, targeting both the heavy and light chains (H+L).

Automatically generated - may contain errors

Lab products found in correlation

Goat anti mouse igg h l hrp, by Bioworld Technology (7 mentions) Anti gapdh, by Bioworld Technology (5 mentions) Anti hif 1α, by Proteintech (4 mentions) β actin, by Proteintech (3 mentions) Pvdf membrane, by Merck Group (3 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Ripa lysis buffer, by Beyotime (2 mentions) Ecl detection reagent, by Merck Group (1 mentions) Bca method, by Thermo Fisher Scientific (1 mentions) Lysis buffer, by Bioss Antibodies (1 mentions) Gapdh polyclonal antibody, by Bioworld Technology (1 mentions) Sds page gel, by Ipsen (1 mentions) 0.45 μm pvdf membrane, by Merck Group (1 mentions) Phosphatase inhibitor, by Solarbio (1 mentions) Omni ecl enhanced pico light chemiluminescence kit, by Ipsen (1 mentions) Chemidoc mp image system, by Bio-Rad (1 mentions) Phenyl methyl sulfonyl fluoride (pmsf), by Solarbio (1 mentions) Bca protein assay kit, by Keygen Biotech (1 mentions) Phosphatase inhibitor, by Beyotime (1 mentions) Enhanced chemiluminescence, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Goat anti-rabbit IgG (H+L) HRP (BS13278), (7 citations) Goat anti-rabbit IgG (H+L) (2 citations) Goat anti-rabbit IgG (H + L)/ HRP antibody (2 citations)

25 protocols using goat anti rabbit igg h l hrp

Hypoxia-Induced HIF-1α and CAIX Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

The variation trend of HIF-1α and CAIX protein expression was studied by western blot (WB). The correlation between myocardial CAIX and HIF-1α could be found. In detail, H9c2 cell hypoxia model was simulated by PA with different concentrations (0-100 μM). Protein was extracted from cells with ice-cold radioimmunoprecipitation assay (RIPA) buffer containing 1 mmol/L phenylmethanesulfonyl uoride (PMSF). The lysates were centrifuged at 12000 g for 15 min at 4 °C. The protein concentration was quanti ed by Bicinchoninic Acid Protein Assay kit (Biosky Biotechnology Corporation, Nanjing, China). Western blot assay was used for protein expression analysis with primary antibody (anti-HIF-1α, proteintech, 1:500, 20960-1-AP; anti-CAIX, proteintech, 1:500, 11071-1-AP; anti-GAPDH, Bioworld
Technology, 1:500, AP0063) and HRP-conjugated secondary antibody (Goat Anti-Rabbit IgG (H+L) HRP, 1:3000, Bioworld Technology, BS13278, or Rabbit Anti-Goat IgG (H+L)-HRP, 1:3000, Bioworld Technology, BS30503). Equal loading was veri ed by incubation with anti-GAPDH. The blots were visualized using ECL, and the signals were quanti ed by densitometry (Image-Pro Plus 6.0).

Zhou W., Zhang B., Fan K., Zhang F., Liu J., & Gou S. (2021). An Original Aspirin-Containing Carbonic Anhydrase 9 Inhibitor Overcomes Hypoxia-induced Drug Resistance to Enhance The Efficacy of Myocardial Protection.

+ Open protocol

+ Expand

Hypoxia-Induced HIF-1α and CAIX Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

Zhou W., Zhang B., Fan K., Yin X., Liu J, & Gou S. (2022). An Original Aspirin-Containing Carbonic Anhydrase 9 Inhibitor Overcomes Hypoxia-Induced Drug Resistance to Enhance the Efficacy of Myocardial Protection. Cardiovascular drugs and therapy, 36(4).

+ Open protocol

+ Expand

Western Blot Analysis of Protein Expression

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total protein was extracted by a previously described method [16 (link)]. Briefly, the protein concentration of F1-treated CAL-62 cells was detected by the BCA method (Thermo Scientific, USA), and the extracts containing equal amounts of proteins (20 μg) were electrophoresed in the desired concentration of polyacrylamide gel according to the size of the target protein and then transferred to a PVDF membrane. The membrane was blocked for 1 h with 5% bovine serum albumin (BSA) in TBS-Tween 20 buffer and incubated with different rabbit monoclonal antibodies (1:1000 dilution; p-AKT, CST #4060S; AKT, CST #4691) and mouse monoclonal antibodies (β-actin, Proteintech, 66009–1-Ig). The membrane was then incubated with anti-rabbit/mouse antibody (1:40,000 dilution; goat anti-rabbit IgG (H + L) HRP, Bioworld, BS13278; goat anti-mouse IgG (H + L) HRP, Bioworld, BS12478) at room temperature for 1 h, and ECL detection reagents (Merck Millipore, USA) were used to visualize the membrane with an image scanner Protein Simple (Santa Clara, CA, United States). Blots were analyzed by Image-Pro Plus Analysis software (Media Cybernetic, Rockville, Maryland, USA).

Lin R., Ma B., Liu N., Zhang L., He T., Liu X., Chen T., Liu W., Liang Y., Wang T., Ni G., Liu X., Yang N., Zhang J, & Yuan J. (2021). Targeted radioimmunotherapy with the iodine-131-labeled caerin 1.1 peptide for human anaplastic thyroid cancer in nude mice. Annals of Nuclear Medicine, 35(7), 811-822.

+ Open protocol

+ Expand

Examining IRF7 and TBK1 Expression in BMDCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

BMDCs seeded at 10⁶ cells/mL were transfected with different plasmids of 2 µg per well, Blank; NSP16; poly (I:C); NSP16+poly (I:C); Pri-NSP16. The cells were harvested and treated with lysis buffer (Bioss, Woburn, MA) at 24 h after transfection. Samples (20 µL) were separated on a 7.5% SDS-PAGE gel (EpiZyme, Cambridge, MA) and blotted onto a 0.45 μm PVDF membrane (Merck Millipore, Germany). Membranes were blocked for 2 h in 5% skim milk in TBST (1 M Tris–HCl saline plus 0.2% Tween-20) and then probed with anti-IRF7 and anti-TBK1 rabbit monoclonal antibodies (Abways, Shanghai, China) at a 1:1,000 dilution for overnight. Membranes were washed (0.2% Tween 20 in 1X TBS) and then probed with goat anti-rabbit IgG (H&L)-HRP (Bioworld, Bloomington, MN) at a 1:3,000 dilution for 2 h before washing and detection with chemiluminescence. The expression of the GAPDH polyclonal antibody (1:10,000) (Bioworld) was also examined in parallel as an internal control.

Wu Y., Li Y., Zhao J., Wu Y., Lu D., Jia J., Chen T., He M., Lin J, & Yang Q. (2023). IBV QX affects the antigen presentation function of BMDCs through nonstructural protein16. Poultry Science, 102(5), 102620.

+ Open protocol

+ Expand

Western Blot Analysis of Neuronal Signaling

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Neurons were lysed at 30 days with radioimmunoprecipitation assay (RIPA) lysis (Solarbio, Cat. #R0010, China), PMSF (Solarbio, Cat. # P0100, China), and phosphatase inhibitor (Solarbio, China) in proportions of 100:1:1, respectively. A BCA Protein Assay Kit (KeyGEN, Cat. #KGP902, China) was used for protein quantification. The proteins were used with 4–20% sodium dodecyl sulfate–polyacrylamide gel for electrophoresis and were transferred into the PVDF membrane (Merck Millipore, Cat. #ISEQ00010, Germany). They were then blocked with 5% nonfat milk for 90 min and incubated overnight with the primary antibody at 4°C. The following day, they were incubated with the secondary antibody. After that, an Omni-ECL™ Enhanced Pico Light Chemiluminescence Kit (EpiZyme, Cat. #SQ101, China) was added to capture the protein with a ChemiDoc™ MP Image System (Bio-RAD, Universal Hood III). The information about the antibodies is as follows: Kv4.3 (1:200; Affinity Biosciences); activating transcription factor 4 (ATF4) (1:200; Cell Signaling Technology); and C/EBP homologous protein (Bushart et al., 2018 (link)) (1:200, Proteintech). Goat anti-mouse IgG (H+L) -HRP (1:10,000; Bioworld) and goat anti-rabbit IgG (H+L) HRP (1:10,000; Bioworld) were used for Western blotting.

Li M., Liu F., Hao X., Fan Y., Li J., Hu Z., Shi J., Fan L., Zhang S., Ma D., Guo M., Xu Y, & Shi C. (2022). Rare KCND3 Loss-of-Function Mutation Associated With the SCA19/22. Frontiers in Molecular Neuroscience, 15, 919199.

+ Open protocol

+ Expand

Western Blot Analysis of Protein Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cells were lysed with lysis buffer (Beyotime, China) freshly supplemented with phosphatase inhibitors and phenylmethanesulfonyl fluoride (Beyotime, China). The samples were fractionated by SDS‐PAGE gels and transferred to microporous polyvinylidene difluoride (PVDF) membranes (Roche, Swiss). The membranes were blocked with 5% skim milk for 1 h and incubated overnight at 4°C with primary antibodies including rabbit polyclonal anti‐AGR2²⁷; ESE1 (Santa Cruz Biotechnology, USA); E‐cadherin (CST#3195, USA), N‐cadherin (CST#13116, USA), and vimentin (CST#5741, USA); GAPDH (10494‐1‐AP, Proteintech, USA). The membranes were then washed and probed with secondary antibodies: goat anti‐mouse IgG (H + L)‐HRP or goat anti‐rabbit IgG (H + L)‐HRP (Bioworld, USA) before detection with the enhanced chemiluminescence (Thermo Fisher Scientific, USA) and imaged using the ChemiDoc XRS + System (Bio‐Rad, USA).

Xu H., Bai J., Tian Y., Feng X., Chen A., Wang J., Wu J., Jin X., Zhang F., Quan M., Chen C., Lee K, & Zhang J. (2022). ESE1/AGR2 axis antagonizes TGF‐β‐induced epithelial‐mesenchymal transition in low‐grade pancreatic cancer. Cancer Medicine, 12(5), 5979-5993.

+ Open protocol

+ Expand

Comparative Analysis of HCC Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

HuH7, HCCLM3 and Hep3B cell lines were obtained from American Type Culture Collection (ATCC) and were maintained in DMEM medium containing 10% fetal bovine serum (Sigma) and 1% Penicillin-Streptomycin (E607011, Sangon Biotech (Shanghai) Co., Ltd). DCZ0415, Mithramycin A, Olaparib were purchased from MedChemExpress (MCE). GAPDH and TRIP13 antibodies were obtained from Proteintech. SP1 and γH2AX antibodies were purchased from ABclonal. Goat Anti-Rabbit IgG (H+L) HRP and Goat Anti-Mouse IgG (H+L) HRP secondary antibodies were obtained from Bioworld.

Xu H., Ma Z., Mo X., Chen X., Xu F., Wu F., Chen H., Zhou G., Xia H, & Zhang C. (2022). Inducing Synergistic DNA Damage by TRIP13 and PARP1 Inhibitors Provides a Potential Treatment for Hepatocellular Carcinoma. Journal of Cancer, 13(7), 2226-2237.

+ Open protocol

+ Expand

Hippocampal Protein Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Each mouse was sacrificed after anesthesia, and the hippocampus was homogenized in Radio immunoprecipitation assay buffer, Radio immunoprecipitation assay (RIPA) lysis buffer reagent (Beyotime Biotechnology, Shanghai, China) using a homogenizer. Notably, 30 μg of total proteins were electrophoresed through 12% Bis-Tris sodium dodecyl sulfate (SDS, CH3(CH2)11OSO3Na) polyacrylamide gels and then transferred to a polyvinyl difluoride (PVDF) membrane. The primary Abs were anti-MTP18 Ab (Proteintech, 14257-1-AP, Rosemont, IL, United States), anti-DRP1 Ab (Abcam, ab184247), and anti-beta-actin Ab (Bioss, bs-0061R, Boston, MA, United States). Goat anti-rabbit IgG (H + L)-HRP (Bioworld, BS13278, Bloomington, IN, United States) was used as the secondary Ab and protein signals were detected using the enhanced chemiluminescence (ECL) system (CWBIO, Taizhou, Jiangsu, China).

Ouattara N., Chen Z., Huang Y., Chen X., Song P., Xiao Z., Li Q., Guan Y., Li Z., Jiang Y., Xu K., Pan S, & Hu Y. (2022). Reduced mitochondrial size in hippocampus and psychiatric behavioral changes in the mutant mice with homologous mutation of Timm8a1-I23fs49X. Frontiers in Cellular Neuroscience, 16, 972964.

+ Open protocol

+ Expand

Protein Extraction and Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total protein was extracted from PDAC cells for Western blot analysis. The cells were lysed with lysis buffer (Beyotime, P0013) containing phosphatase inhibitors and phenylmethanesulfonyl fluoride (PMSF, Beyotime, ST506). The samples were fractionated by SDS-PAGE gels and transferred to microporous polyvinylidene difluoride (PVDF) membranes (Roche, 3010040001). The membranes were blocked with 5% skim milk for 1 h and incubated with primary antibodies overnight at 4°C. Antibodies against GAPDH (10494-1-AP) and anti-β-actin (20536-1-AP) were purchased from Proteintech. Other primary antibodies including anti-YAP (CST, 14074), anti-E-Cadherin (CST, 3195), anti-Vimentin (CST, 5741), anti-phospho-Smad2 (CST, 8828), anti-Smad2/3 (CST, 8685), anti-phospho AKT (CST, 4060), and anti-AKT (CST, 9272) were the products of Cell Signaling Technology. The membranes were then probed with secondary antibodies: goat anti-mouse IgG (H+L)-HRP or goat anti-rabbit IgG (H+L)-HRP (Bioworld).

Gao C., Quan M.Y., Chen Q.J., Yang R., Wu Y., Liu J.Y., Lin Z.Y., Li X., Cai J.T., Jiang T.F., Xu L., Mossahebi-Mohammadi M., Guo Q, & Zhang J.S. (2021). Yap1-2 Isoform Is the Primary Mediator in TGF-β1 Induced EMT in Pancreatic Cancer. Frontiers in Oncology, 11, 649290.

+ Open protocol

+ Expand

Protein Extraction and Western Blotting

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were lysed in RIPA buffer (Beyotime, China) with protease inhibitor cocktail (Bimake, USA) and PMSF (GBCBIO Technologies, China) on ice for 30 min. After the supernatant was harvested and total protein concentration was determined by a BCA protein assay kit (Bioworld, USA), protein lysates were separated by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (Merck, Germany) via semi-dry transfer (Bio-Rad, USA). The membranes were blocked with 5% non-fat milk in PBST for 2 h at room temperature before incubating with specific primary antibodies, followed by Goat anti-Rabbit IgG (H+L)-HRP or Goat anti-Mouse IgG (H+L)-HRP (Bioworld, USA), respectively. The following primary antibodies were used: β-actin, Myc-tag (Proteintech, China), EGFP (abcam, UK), and anti-HSP90B1 (Proteintech, China).

Chen J., Lu Z., Yang X., Zhou Y., Gao J., Zhang S., Huang S., Cai J., Yu J., Zhao W, & Zhang B. (2022). Severe Acute Respiratory Syndrome Coronavirus 2 ORF8 Protein Inhibits Type I Interferon Production by Targeting HSP90B1 Signaling. Frontiers in Cellular and Infection Microbiology, 12, 899546.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!