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Sc9158 is a laboratory equipment designed for scientific research. It serves as a precision instrument for various applications in the field of biotechnology. The core function of Sc9158 is to provide accurate and reliable data for researchers.

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Lab products found in correlation

Sc 6236, by Santa Cruz Biotechnology (1 mentions) Versadoc system, by Bio-Rad (1 mentions) Horseradish peroxidase conjugated secondary antibody, by Bio-Rad (1 mentions) Sc 7382, by Santa Cruz Biotechnology (1 mentions) G box f3 genesys, by Syngene (1 mentions)

2 protocols using sc9158

1

Extracellular Vesicle Protein Analysis

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Cell and tissue lysates were prepared as described previously (28 (link)). EV pellets were lysed in a non-reducing loading buffer. Primary antibodies against CD63 (1:500, AB0047, Sicgen), CD81 (1:250, Sc9158, Santa Cruz Biotechnology), Cx43 (1:2,500, AB0016, Sicgen), Calnexin (1:5,000, AB0041, Sicgen), phosphorylated and total p44/42 (1:500, 4377S and 9102, Cell Signaling Technology), Bax (1:250, Sc6236, Santa Cruz Biotechnology) and Bcl2 (1:250, Sc7382, Santa Cruz Biotechnology) were used, followed by incubation with horseradish peroxidase–conjugated secondary antibodies (1:10,000, Bio-Rad). Images were visualized in a VersaDoc system (Bio-Rad). Densitometric quantification was performed in unsaturated images using ImageJ.
Martins-Marques T., Pinho M.J., Zuzarte M., Oliveira C., Pereira P., Sluijter J.P., Gomes C, & Girao H. (2016). Presence of Cx43 in extracellular vesicles reduces the cardiotoxicity of the anti-tumour therapeutic approach with doxorubicin. Journal of Extracellular Vesicles, 5, 10.3402/jev.v5.32538.
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2

Exosome Protein Characterization by Immunoblotting

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Analysis of exosomes by immunoblotting was performed using standard protocols: Proteins were denatured, separated on 4–12% polyacrylamide gels, transferred onto a nitrocellulose membrane and probed with antibodies against heat shock protein 70 (HSP70, 1:100 Santa Cruz Biotechnology, sc-1060), and tetraspanins CD9 (1:100 MM2/57, Millipore CBL-162) and CD81 (1:100 Santa Cruz Biotechnology, sc-9158) followed by appropriate horseradish peroxidase (HRP) conjugated secondary antibodies against the primary antibody (all secondary antibodies were from Dako Agilent). ASC lysates were used as the positive control. The blots were then incubated with a chemiluminescent HRP substrate and detected with G:BOX F3 GeneSys (Syngene, UK).
Bonafede R., Brandi J., Manfredi M., Scambi I., Schiaffino L., Merigo F., Turano E., Bonetti B., Marengo E., Cecconi D, & Mariotti R. (2019). The Anti-Apoptotic Effect of ASC-Exosomes in an In Vitro ALS Model and Their Proteomic Analysis. Cells, 8(9), 1087.
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